A5100388340- Plant Molecular Biology Research
- Plant Reproductive Biology
- Cellular Mechanics and Interactions
- Polysaccharides and Plant Cell Walls
- Photosynthetic Processes and Mechanisms
- PI3K/AKT/mTOR signaling in cancer
- Plant nutrient uptake and metabolism
- Plant Gene Expression Analysis
- Light effects on plants
- Plant Stress Responses and Tolerance
- Phytochemistry and Biological Activities
- Plant and animal studies
- Leaf Properties and Growth Measurement
- Cancer Cells and Metastasis
- Cell Adhesion Molecules Research
- 3D Printing in Biomedical Research
- Force Microscopy Techniques and Applications
- Natural product bioactivities and synthesis
- Plant biochemistry and biosynthesis
- Ubiquitin and proteasome pathways
- Smart Agriculture and AI
- Biofuel production and bioconversion
- Spectroscopy and Chemometric Analyses
- Plant-Microbe Interactions and Immunity
- Lung Cancer Treatments and Mutations
Beijing Normal University
2013-2025
Southwest University
2008-2025
Zhengzhou University
2017-2025
Southwest Hospital
2024
Tianjin University of Traditional Chinese Medicine
2017-2024
Qingdao Agricultural University
2024
Institute for Advanced Study
2024
Army Medical University
2024
Agricultural Genomics Institute at Shenzhen
2023
Chinese Academy of Agricultural Sciences
2023
Auxin binding protein 1 (ABP1) has been studied for decades. It suggested that ABP1 functions as an auxin receptor and essential role in many developmental processes. Here we present our unexpected findings is neither required signaling nor necessary plant development under normal growth conditions. We used ribozyme-based CRISPR technology to generate Arabidopsis abp1 mutant contains a 5-bp deletion the first exon of ABP1, which resulted frameshift introduction early stop codons. also...
The target of rapamycin (TOR) pathway is a major regulator growth in all eukaryotes, integrating energy, nutrient and stress signals into molecular decisions. By using large-scale MS-based metabolite profiling primary, secondary lipid compounds combination with array-based transcript profiling, we show that the TOR protein not only regulates but also influences partitioning central energy metabolism. study was performed on plants exhibiting conditional down-regulation AtTOR expression,...
Plant hormones are small-molecule signaling compounds that collectively involved in all aspects of plant growth and development. Unlike animals, plants actively regulate the spatial distribution several their hormones. For example, auxin transport results formation maxima have a key role developmental patterning. However, other hormones, including gibberellic acid (GA), is largely unknown. To address this, we generated two bioactive fluorescent GA studied Arabidopsis thaliana roots. The...
Gibberellins (GAs) are plant hormones that promote a wide range of developmental processes. While GA signalling is well understood, little known about how transported or distribution regulated. Here we utilize fluorescently labelled GAs (GA-Fl) to screen for Arabidopsis mutants deficient in transport. We show the NPF3 transporter efficiently transports across cell membranes vitro and GA-Fl vivo. expressed root endodermis repressed by GA. targeted plasma membrane subject rapid BFA-dependent...
The TIR1/AFB auxin co-receptors mediate diverse responses to the plant hormone auxin. Arabidopsis genome encodes six proteins representing three of four clades that were established prior angiosperm radiation. To determine role these in development we performed an extensive genetic analysis involving generation and characterization all possible multiply-mutant lines. We find loss results early embryo defects eventually seed abortion, yet a single wild-type allele TIR1 or AFB2 is sufficient...
Abstract Plastic debris widely pollutes freshwaters. Abiotic and biotic degradation of plastics releases carbon-based substrates that are available for heterotrophic growth, but little is known about how these novel organic compounds influence microbial metabolism. Here we found leachate from plastic shopping bags was chemically distinct more bioavailable than natural matter 29 Scandinavian lakes. Consequently, increased bacterial biomass acquisition by 2.29-times when added at an...
Abstract Background The phenotypic traits of leaves are the direct reflection agronomic in growth process leafy vegetables, which plays a vital role selection high-quality vegetable varieties. current image-based extraction research mainly focuses on morphological and structural plants or leaves, there few studies phenotypes physiological leaves. has developed deep learning model aimed at predicting total chlorophyll greenhouse lettuce directly from full spectrum hyperspectral images....
The native auxin, indole-3-acetic acid (IAA), is a major regulator of plant growth and development. Its nonuniform distribution between cells tissues underlies the spatiotemporal coordination many developmental events responses to environmental stimuli. regulation auxin gradients formation maxima/minima most likely involve both metabolic transport processes. In this article, we have demonstrated that 2-oxindole-3-acetic (oxIAA) primary IAA catabolite formed in Arabidopsis thaliana root...
Abstract The plant hormone auxin is perceived by a family of F-box proteins called the TIR1/AFBs. Phylogenetic studies reveal that these fall into four clades in flowering plants TIR1, AFB2, AFB4, and AFB6. Genetic indicate members TIR1 AFB2 groups act as positive regulators signaling promoting degradation Aux/IAA transcriptional repressors. In this report, we demonstrate both AFB4 AFB5 also function receptors based on vitro assays. We provide genetic evidence are targets picloram auxinic...
Abstract Autapses are synaptic contacts of a neuron’s axon onto its own dendrite and soma. In the neocortex, self-inhibiting autapses in GABAergic interneurons abundant number play critical roles regulating spike precision network activity. Here we examine whether principal glutamatergic pyramidal cells (PCs) also form functional autapses. patch-clamp recording from both rodent human PCs, isolated autaptic responses found that these occur predominantly layer-5 PCs projecting to subcortical...
Multicellular organisms contain a large number of formins; however, their physiological roles in plants remain poorly understood. Here, we reveal that formin homology 5 (FH5), type II mutated rice morphology determinant (rmd), plays crucial role determining (Oryza sativa) morphology. FH5/RMD encodes formin-like protein consisting an N-terminal phosphatase tensin (PTEN)-like domain, FH1 and FH2 domain. The rmd mutants display bending growth pattern seedlings, are stunted as adult plants, have...
• Villin is one of the major actin filament bundling proteins in plants. The function Arabidopsis VILLINs (AtVLNs) still poorly understood living cells. In this report, biochemical activity and cellular AtVLN4 were examined. property was characterized by co-sedimentation assays, fluorescence microscopy spectroscopy pyrene fluorescence. vivo analysed ectopically expressing it tobacco pollen examining phenotypes its T-DNA insertional Recombinant protein exhibited multiple activities on actin,...
The evolution of the plant vasculature was essential for emergence terrestrial life. Xylem vessels are solute-transporting elements in that possess secondary wall thickenings deposited intricate patterns. Evenly dispersed microtubule (MT) bands support formation these thickenings, but how MTs direct cell synthesis during this process remains largely unknown. Cellulose is major constituent and synthesized by plasma membrane-localized cellulose synthases (CesAs) whose catalytic activity...
An ABC transporter gene ( OsABCG15 ) was proven to be involved in pollen development rice. The corresponding protein localized on the plasma membrane using subcellular localization. Wax, cutin, and sporopollenin are important for normal of anther cuticle exine, respectively. Their lipid soluble precursors, which produced tapetum, then secreted transferred microspore surface polymerization. However, little is known about mechanisms underlying transport these precursors. Here, we identified...
Hormone-sensitive lipases (HSLs) are widely distributed in microorganisms, plants, and animals. Microbial HSLs classified into two subfamilies, an unnamed new subfamily the GDSAG motif subfamily. Due to lack of structural information, detailed catalytic mechanism is not yet clarified. Based on sequence analysis, we propose name as GTSAG We identified a novel HSL esterase E25, member subfamily, by functional metagenomic screening, resolved its structure at 2.05 Å. E25 mesophilic (optimum...
Abstract Cancer metastasis is the most deadly stage in cancer progression. Despite significant efforts over past decades, it remains elusive why only a very small fraction of cells able to generate micrometastasis and metastatic colonization. Recently we have shown that tumor-repopulating (TRCs), highly tumorigenic subpopulation mouse melanoma cells, can be selected by being cultured grown 3D soft fibrin gels. Here show when injected into yolk 2 day-post-fertilization (dpf) embryo Tg...