A5100603591- Immune cells in cancer
- Cancer, Hypoxia, and Metabolism
- Immune Cell Function and Interaction
- Autophagy in Disease and Therapy
- Osteoarthritis Treatment and Mechanisms
- CAR-T cell therapy research
- Scoliosis diagnosis and treatment
- Immunotherapy and Immune Responses
- Extracellular vesicles in disease
- Cancer Cells and Metastasis
- Inflammatory mediators and NSAID effects
- MicroRNA in disease regulation
- Cholesterol and Lipid Metabolism
- Liver Disease Diagnosis and Treatment
- RNA Interference and Gene Delivery
- T-cell and B-cell Immunology
- Pancreatic function and diabetes
- Spinal Fractures and Fixation Techniques
- Phagocytosis and Immune Regulation
- Metabolomics and Mass Spectrometry Studies
- Bone fractures and treatments
- RNA Research and Splicing
- RNA modifications and cancer
- Tryptophan and brain disorders
- Epigenetics and DNA Methylation
Huazhong University of Science and Technology
2012-2025
Tianjin Medical University General Hospital
2017-2025
Tongji Hospital
2022-2024
First Affiliated Hospital of Zhengzhou University
2020-2022
Anhui Provincial Hospital
2019
University of Science and Technology of China
2019
Hefei National Center for Physical Sciences at Nanoscale
2019
Renji Hospital
2017
Society for Conservation Biology
2017
Zhengzhou City Hospital
2017
Autophagy is a process by which cytoplasmic organelles can be catabolized either to remove defective structures or as means of providing macromolecules for energy generation under conditions nutrient starvation. In this study we demonstrate that mitochondrial autophagy induced hypoxia, requires the hypoxia-dependent factor-1-dependent expression BNIP3 and constitutive Beclin-1 Atg5, in cells subjected prolonged an adaptive metabolic response necessary prevent increased levels reactive oxygen...
Abstract The steady flow of lactic acid (LA) from tumor cells to the extracellular space via monocarboxylate transporter symport system suppresses antitumor T cell immunity. However, LA is a natural energy metabolite that can be oxidized in mitochondria and could potentially stimulate cells. Here we show lactate-lowering mood stabilizer lithium carbonate (LC) inhibit LA-mediated CD8 + immunosuppression. Cytoplasmic increased pumping protons into lysosomes. LC interfered with vacuolar ATPase...
The identification of mechanisms to store glucose carbon in the form glycogen rather than fat hepatocytes has important implications for prevention nonalcoholic fatty liver disease (NAFLD) and other chronic metabolic diseases. In this work, we show that glycogenesis uses its intermediate metabolite uridine diphosphate (UDPG) antagonize lipogenesis, thus steering both mouse human toward storing as glycogen. underlying mechanism involves transport UDPG Golgi apparatus, where it binds site-1...
In recent years, researchers have examined the use of polyunsaturated fatty acids (PUFA) or a low omega-6/3 ratio to protect knee joint. The current study is based on genome-wide association (GWAS) analysis and uses Mendelian randomization (MR) method evaluate effect total PUFA, omega-3, omega-6, ratios osteoarthritis (OA). First, we downloaded latest PUFA OA GWAS data. data were divided into four groups: ratios. split nine hip (total, males, females), hand females). Then, qualified SNPs...
Abstract Macrophages in tumors (tumor-associated macrophages, TAMs), a major population within most tumors, play key homeostatic functions by stimulating angiogenesis, enhancing tumor cell growth, and suppressing antitumor immunity. Resetting TAMs simple, efficacious safe approach(s) is highly desirable to enhance immunity attenuate malignancy. Previously, we used cell-derived microparticles package chemotherapeutic drugs (drug-MPs), which resulted significant treatment outcome human...
Abstract Mechanical force contributes to perforin pore formation at immune synapses, thus facilitating the cytotoxic T lymphocytes (CTL)-mediated killing of tumor cells in a unidirectional fashion. How such mechanical cues affect CTL evasion perforin-mediated autolysis remains unclear. Here we show that activated CTLs use their softness evade autolysis, which, however, is shared by leukemic killing. Downregulation filamin A identified induce via ZAP70-mediated YAP Y357 phosphorylation and...
Clinical applications of antiangiogenic agents profoundly affect tumor cell behaviors via the resultant hypoxia. To date, how hypoxia regulates cells remains unclear. Here, we show that promotes growth human breast tumorigenic repopulate tumors [tumor-repopulating (TRCs)] in vitro and vivo. This stimulating effect is ascribed to hypoxia-induced reactive oxygen species (ROS) activates Akt NF-κB, dependent on attenuated tricarboxylic acid (TCA) cycle. We find fumarate accumulated TCA cycle...
Pancreatic ductal adenocarcinoma (PDAC) originates from normal pancreatic ducts where digestive juice is regularly produced. It remains unclear how PDAC can escape autodigestion by enzymes. Here we show that human tumour cells use gasdermin E (GSDME), a pore-forming protein, to mediate resistance. GSDME facilitates the express mucin 1 and 13, which form barrier prevent chymotrypsin-mediated destruction. Inoculation of GSDME-/- results in subcutaneous but not orthotopic formation mice....
Glycolysis facilitates the rapid recall response of CD8+ memory T (Tm) cells. However, it remains unclear whether Tm cells uptake exogenous glucose or mobilize endogenous sugar to fuel glycolysis. Here, we show that intracellular glycogen rather than extracellular acts as major carbon source for early response. Following antigenic stimulation, exhibit high phosphorylase (brain form, PYGB) activity, leading glycogenolysis and release glucose-6-phosphate (G6P). Elevated G6P mainly flows...
Weak immunogenicity of tumor cells is a root cause for the ultimate failure immunosurveillance and immunotherapy. Although evolution can be shaped by immunoediting toward less immunogenic phenotype, mechanisms governing initial primordial or original cancer stem remain obscure. Here, using single tumor-repopulating cell (TRC) to form tumors in immunodeficient immunocompetent mice, we demonstrated that heterogeneity an inherent trait tumorigenic defined activation status signal transducer...
Identification of mechanisms that program early effector T cells to either terminal (T eff ) or memory m has important implications for protective immunity against infections and cancers. Here, we show the cytosolic transcription factor aryl hydrocarbon receptor (AhR) is used by fate. Upon antigen engagement, AhR rapidly up-regulated via reactive oxygen species signaling in CD8 + cells, which does not affect response, but required formation. Mechanistically, activated up-regulate HIF-1α...
Abstract DIS3-like 3′-5′ exoribonuclease 2 (DIS3L2) degrades aberrant RNAs, however, its function in tumorigenesis remains largely unexplored. Here, DIS3L2 expression promoted human hepatocellular carcinoma (HCC) progression via heterogeneous nuclear ribonucleoproteins (hnRNP) U-mediated alternative splicing. directly interacted with hnRNP U through cold-shock domains and inclusion of exon 3b during splicing pre-Rac1 independent exonuclease activity, yielding an oncogenic variant, Rac1b,...
Tumor-derived factors are thought to regulate thrombocytosis and erythrocytopenia in individuals with cancer; however, such have not yet been identified. Here we show that tumor cell-released kynurenine (Kyn) biases megakaryocytic-erythroid progenitor cell (MEP) differentiation into megakaryocytes cancer by activating the aryl hydrocarbon receptor-Runt-related transcription factor 1 (AhR-RUNX1) axis. During growth, large amounts of Kyn from cells released periphery, where they taken up MEPs...
Tumor cells expressing TLRs is generally recognized to mediate tumor inflammation. However, whether and how TLR signaling pathways negatively regulate inflammation remains unclear. In this report, we find that TLR4 of H22 hepatocarcinoma transduced through MyD88 pathway actin cytoskeletons, leading the release microparticles (MPs), cellular membrane-derived vesicles. As a result, macrophages take up MPs acquire MP-contained microRNA let-7b, which attenuates by targeting proinflammatory...
It is currently uncertain whether smoking a risk factor for carpal tunnel syndrome (CTS). This study aims to elucidate association between and CTS using Mendelian randomization (MR) analysis. was secondary analysis of publicly available GWAS data, four phenotypes (smoking initiation, status, lifetime smoking, never smoking) as exposures, two datasets (discovery validation sets) outcomes MR The discovery set (n=480201) used explore the causal relationship CTS, while (n=385304) confirm...
Recent studies highlight the role of H-type vasculature in bone regeneration. This study, based on single-cell RNA sequencing (scRNA-seq), aims to explore changes endothelial cells (H_ECs) osteonecrosis femoral head (ONFH) and hip osteoarthritis (HOA), focusing death modes such as ferroptosis, pyroptosis, parthanatos. We re-analyzed scRNA-seq data samples publicly available 2022. study selected nine (3 each from HOA, ONFH stage 3 A, 4). CD31 + EMCN were classified H_ECs. Molecular...
The tumor microenvironment predominantly polarizes tumor-associated macrophages (TAMs) toward an M2-like phenotype, thereby inhibiting antitumor immune responses. This process is substantially affected by metabolic reprogramming; however, reeducating TAMs to enhance their capabilities through remodeling remains a challenge. Here, we show that tumor-derived microparticles loaded with succinate (SMPs) can remodel the state of TAMs. SMPs promote classical M1-like polarization enhancing...