A5100685990- Cancer, Hypoxia, and Metabolism
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Immune cells in cancer
- Circular RNAs in diseases
- Extracellular vesicles in disease
- Cellular Mechanics and Interactions
- Immune Cell Function and Interaction
- RNA Research and Splicing
- COVID-19 Clinical Research Studies
- Epigenetics and DNA Methylation
- Cancer Cells and Metastasis
- Diet and metabolism studies
- CAR-T cell therapy research
- Mitochondrial Function and Pathology
- Cancer Immunotherapy and Biomarkers
- Inflammasome and immune disorders
- Pancreatic function and diabetes
- Luminescence and Fluorescent Materials
- Caveolin-1 and cellular processes
- Immunotherapy and Immune Responses
- Traditional Chinese Medicine Analysis
- Cancer-related gene regulation
- SARS-CoV-2 and COVID-19 Research
- Molecular Sensors and Ion Detection
Xuzhou Medical College
2022-2025
Hebei University of Technology
2023-2025
Sun Yat-sen University Cancer Center
2019-2025
State Key Laboratory of Oncology in South China
2020-2025
Sun Yat-sen University
2019-2025
Shanghai Jiao Tong University
2017-2025
Renji Hospital
2025
Peking University
2008-2024
Peking University Third Hospital
2024
Beihang University
2005-2024
Cytokine release syndrome (CRS) counteracts the effectiveness of chimeric antigen receptor (CAR) T cell therapy in cancer patients, but mechanism underlying CRS remains unclear. Here, we show that tumor pyroptosis triggers during CAR therapy. We find cells rapidly activate caspase 3 target through granzyme B. The latter cleaves gasdermin E (GSDME), a pore-forming protein highly expressed B leukemic and other cells, which results extensive pyroptosis. Consequently, pyroptosis-released factors...
Abstract Resetting tumor-associated macrophages (TAMs) is a promising strategy to ameliorate the immunosuppressive tumor microenvironment and improve innate adaptive antitumor immunity. Here we show that chloroquine (CQ), proven anti-malarial drug, can function as an immune modulator switches TAMs from M2 tumor-killing M1 phenotype. Mechanistically, CQ increases macrophage lysosomal pH, causing Ca 2+ release via channel mucolipin-1 (Mcoln1), which induces activation of p38 NF-κB, thus...
Circulating tumor DNA methylation–based signatures are potential biomarkers for early diagnosis and prognosis of colorectal cancer.
Interactions with the immune system may lead tumorigenic cells into dormancy. However, underlying molecular mechanism is poorly understood. Using a 3D fibrin gel model, we show that IFN-γ induces tumour-repopulating (TRCs) to enter dormancy through an indolamine 2,3-dioxygenase 1 (IDO1)-kynurenine (Kyn)-aryl hydrocarbon receptor (AhR)-p27 dependent pathway. Mechanistically, signalling triggers differentiated tumour cell apoptosis via STAT1; however, when IDO1 and AhR are highly expressed as...
Abstract The existence of breast cancer stem cells (BCSCs) is a major reason underlying metastasis and recurrence after chemotherapy radiotherapy. Targeting BCSCs may ameliorate relapse therapy resistance. Here we report that expression the pseudokinase Tribble 3 (TRIB3) positively associates with stemness progression. Elevated TRIB3 supports by interacting AKT to interfere FOXO1-AKT interaction suppress FOXO1 phosphorylation, ubiquitination, degradation E3 ligases SKP2 NEDD4L. accumulated...
Abstract Our current understanding of how sugar metabolism affects inflammatory pathways in macrophages is incomplete. Here, we show that glycogen an important event controls macrophage-mediated responses. IFN-γ/LPS treatment stimulates to synthesize glycogen, which then channeled through glycogenolysis generate G6P and further the pentose phosphate pathway yield abundant NADPH, ensuring high levels reduced glutathione for macrophage survival. Meanwhile, also increases UDPG receptor P2Y 14...
Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) invades the alveoli, where abundant alveolar macrophages (AMs) reside. How AMs respond to SARS-CoV-2 invasion remains elusive. Here, we show that classically activated M1 facilitate viral spread; however, alternatively M2 limit spread. utilize cellular softness efficiently take up SARS-CoV-2. Subsequently, invaded viruses over endo-lysosomal system escape. have a lower endosomal pH, favoring membrane fusion and allowing...
Background Colorectal cancer (CRC) is among the most common malignant cancers worldwide, and its development influenced by inflammation, nutrition, immune status. Therefore, we combined C-reactive protein (CRP), albumin, lymphocyte, which could reflect above status, to be CRP-albumin-lymphocyte (CALLY) index, evaluated association with overall survival (OS) in patients CRC. Methods The clinicopathological laboratory characteristics of 1260 CRC were collected from Investigation on Nutrition...
Silent hypoxia has emerged as a unique feature of coronavirus disease 2019 (COVID-19). In this study, we show that mucins are accumulated in the bronchoalveolar lavage fluid (BALF) COVID-19 patients and upregulated lungs severe respiratory syndrome 2 (SARS-CoV-2)-infected mice macaques. We find induction either interferon (IFN)-β or IFN-γ upon SARS-CoV-2 infection results activation aryl hydrocarbon receptor (AhR) signaling through an IDO-Kyn-dependent pathway, leading to transcriptional...
Article4 December 2020Open Access Source DataTransparent process Cell softness regulates tumorigenicity and stemness of cancer cells Jiadi Lv Department Immunology & National Key Laboratory Medical Molecular Biology, Institute Basic Sciences, Chinese Academy Sciences (CAMS) Peking Union College, Beijing, ChinaThese authors contributed equally to this work Search for more papers by author Yaoping Liu Microelectronics, University, Feiran Cheng orcid.org/0000-0001-9460-9949 Jiping Li Beijing...
Dynamic interaction with the immune system profoundly regulates tumor cell dormancy. However, it is unclear how immunological cues trigger cancer cell–intrinsic signaling pathways for entering into Here, we show that IFN-β treatment induced tumor-repopulating cells (TRC) to enter dormancy through an indolamine 2,3-dioxygenase/kynurenine/aryl hydrocarbon receptor/p27–dependent (IDO/Kyn/AhR/p27-dependent) pathway. Strategies block this metabolic circuitry did not relieve dormancy, but led...
Abstract Dormancy is recognized as a critical biological event for tumorigenic cells surviving in an extremely harsh environment. Understanding the molecular process of dormancy can unlock novel approaches to tackle cancers. We recently reported that stem-like tumor-repopulating (TRC) sense mechanical signals and rapidly proliferate 90 Pa soft fibrin matrix. Here, we show stiff environment induces TRC via epigenetic program initiated by translocation Cdc42, cytosolic regulator...
Abstract Biomechanics is a fundamental feature of cell. However, the manner by which actomysin tension affects tumor immune evasion remains unclear. Here we show that although cytotoxic T lymphocytes (CTL) can effectively destroy stiff differentiated cells, they fail to kill soft tumor-repopulating cells (TRC). TRC softness prevented membrane pore formation caused CTL-released perforin. Perforin interacting with nonmuscle myosin heavy-chain 9 transmitted forces less F-actins in TRC, thus...
Abstract Hypoxia is known to be commonly present in breast tumor microenvironments. Stem-like cells that repopulate tumors, termed tumor-repopulating (TRC), thrive under hypoxic conditions, but the underlying mechanism remains unclear. Here, we show hypoxia promotes growth of TRCs through metabolic reprogramming. mobilized transcription factors HIF1α and FoxO1 induced epigenetic reprogramming upregulate cytosolic phosphoenolpyruvate carboxykinase (PCK1), a key enzyme initiates...
Clear cell renal carcinoma (ccRCC) is a common malignant tumour of the urinary tract. The major causes poor prognosis are lack early diagnosis and metastasis. Accumulating research reveals that circular RNAs (circRNAs) can play key roles in development progression cancer. However, role circRNAs ccRCC still uncertain.The microarray (n = 4) was performed to investigate with differential expression tissues. candidate circRNA selected based on cut-off criteria, such as abundance, size design...
Abstract Background Gastric cancer (GC) is one of the most common malignant tumors in China. Circular RNAs (circRNAs) are novel non-coding with important regulatory roles progression. IGF2BP3 has been found to play oncogenic various cancers including GC, while exact mechanism largely unknown. Methods The expression GC was evaluated by Western Blot and bioinformatics analysis. CircRNA profiles were screened via RIP-seq GC. Sanger sequencing, RNase R digestion, nucleo-plasmic separation...
Abstract Background Insulin like growth factor II mRNA binding protein 3 (IGF2BP3) is an RNA with multiple roles in regulation of gene expression at the post-transcriptional level and implicated tumorigenesis progression numerous cancers including gastric cancer (GC). Circular RNAs (circRNAs) are a diverse endogenous noncoding population that have important regulatory cancer. However, circRNAs regulate IGF2BP3 GC largely unknown. Methods CircRNAs bound to were screened cells using...
Abstract Background Clear cell renal carcinoma (ccRCC) is the most prevalent kidney cancer with high aggressive phenotype and poor prognosis. Accumulating evidence suggests that circRNAs have been identified as pivotal mediators in cancers. However, role of ccRCC progression remains elusive. Methods The differentially expressed 4 paired human adjacent noncancerous tissues were screened using circRNA microarrays candidate target was selected based on expression level weighted gene correlation...