A5050588060- Cancer-related molecular mechanisms research
- Lung Cancer Treatments and Mutations
- Ferroptosis and cancer prognosis
- Lung Cancer Diagnosis and Treatment
- MicroRNA in disease regulation
- Extracellular vesicles in disease
- RNA modifications and cancer
- Pancreatic and Hepatic Oncology Research
- Advanced biosensing and bioanalysis techniques
- Ovarian cancer diagnosis and treatment
- Immune Cell Function and Interaction
- Inflammatory Biomarkers in Disease Prognosis
- Lung Cancer Research Studies
- Cancer Treatment and Pharmacology
- Protease and Inhibitor Mechanisms
- Radiomics and Machine Learning in Medical Imaging
- Peptidase Inhibition and Analysis
- Cancer Immunotherapy and Biomarkers
- Colorectal Cancer Screening and Detection
- Medical Imaging and Pathology Studies
- Circular RNAs in diseases
- RNA Interference and Gene Delivery
- Galectins and Cancer Biology
- Metabolomics and Mass Spectrometry Studies
- Advanced Proteomics Techniques and Applications
Sun Yat-sen University
2015-2024
Sun Yat-sen University Cancer Center
2015-2024
Wuhan University
2024
Wuhan Children's Hospital
2024
Huazhong University of Science and Technology
2024
Peking Union Medical College Hospital
2024
Zhongnan Hospital of Wuhan University
2024
Tsinghua University
2024
Center for Life Sciences
2024
State Key Laboratory of Membrane Biology
2024
Circulating tumor DNA methylation–based signatures are potential biomarkers for early diagnosis and prognosis of colorectal cancer.
Disulfiram/copper (DSF/Cu) is a promising antitumor reagent for clinical application due to its excellent anticancer activity and safety. However, the mechanism of DSF/Cu has not been fully elucidated. Our study showed that strongly induced cytotoxic effects on both nasopharyngeal carcinoma (NPC) cells α-smooth muscle actin (α-SMA)-positive fibroblasts. Fluorescence activated cell sorting (FACS) analysis further higher late apoptosis rate in α-SMA-positive fibroblasts than tumor cells,...
Objective Exosomes released from tumour cells are packed with unique RNA and protein cargo, they emerging as an important mediator in the communication network that promotes progression. The facultative intracellular bacterium Fusobacterium nucleatum (Fn) is colorectal cancer (CRC)-associated bacterium. To date, function of exosomes Fn-infected CRC has not been explored. Design were isolated by sequential differential centrifugation verified transmission electron microscopy, NanoSight...
Gut microbial dysbiosis contributes to the development of colorectal cancer (CRC). We evaluated utility fecal bacterial biomarker candidates identified by our 16S rDNA sequencing analysis for CRC diagnosis.We measured relative abundance Fusobacterium nucleatum (Fn), Faecalibacterium prausnitzii (Fp), Bifidobacterium (Bb), and Lactobacillus (Lb) quantitative PCR in samples from 2 cohorts 903 individuals. validated diagnostic performance these ratios investigated antagonistic effect Fn against...
Fusobacterium nucleatum (F. nucleatum, Fn) is associated with the colorectal cancer (CRC). Fn-infection could induce significant levels of serum Fn-specific antibodies in human and mice. The objective this study was to identify that elicit a humoral response patients CRC evaluate diagnostic performance anti-Fn antibodies. In work, we showed mean absorbance value anti-Fn-IgA -IgG group were significantly higher than those benign colon disease healthy control (P < 0.001). sensitivity...
MicroRNAs (miRNAs) show considerable promise as therapeutic agents to improve tumor treatment, they have been revealed crucial modulators in progression. However, our understanding of their roles gastric carcinoma (GC) metastasis is limited. Here, we aimed identify novel miRNAs involved GC and explored regulatory mechanisms significance GC.
Currently, the influence of tumor microbiome on effectiveness immunotherapy remains largely unknown. Intratumoural Fusobacterium nucleatum (Fn) functions as an oncogenic bacterium and can promote progression in esophageal squamous cell carcinoma (ESCC). Our previous study revealed that Fn is a facultative intracellular its virulence factor Fn-Dps facilitates survival Fn. In this study, we find DNA enriched nonresponder (NR) group among ESCC patients receiving PD-1 inhibitor serum antibody...
The tumor-associated microbiota plays a vital role in cancer development. Accumulating evidence shows that Fusobacterium nucleatum (Fn) participates the progression of multiple tumor types. However, underlying mechanisms remain unclear. This study examined expression methyltransferase-like protein 3 (METTL3) during Fn infection and elucidated function pathway Fn-induced m6A methylation esophageal squamous cell carcinoma (ESCC). abundance patient tissues was determined by qPCR. Western blot,...
Untargeted metabolomic analysis using mass spectrometry provides comprehensive metabolic profiling, but its medical application faces challenges of complex data processing, high inter-batch variability, and unidentified metabolites. Here, we present DeepMSProfiler, an explainable deep-learning-based method, enabling end-to-end on raw signals with output accuracy reliability. Using cross-hospital 859 human serum samples from lung adenocarcinoma, benign nodules, healthy individuals,...
Background & AimsThe liver is an organ with paradoxic immunologic properties and known for its tolerant microenvironment, which holds important implications hepatic diseases. The molecular basis this local immune suppression, however, poorly understood. In study, we aimed to determine the role of sinusoidal endothelial cell lectin (LSECtin), a recently identified member dendritic cell-specific ICAM-3 grabbing nonintegrin (DC-SIGN) family, in regulation T-cell response.MethodsThe effector...
Although the alterations of lipid profile in lung cancer have been documented, prognostic value serum HDL-C level and its correlation with inflammation NSCLC remain unknown.Levels preoperative concentrations (including HDL-C, LDL-C, TC, TG) inflammatory biomarker C-reactive protein (CRP) were retrospectively analyzed 228 patients 300 healthy controls. The levels these two populations compared. Univariate multivariate cox hazards analyses performed to investigate NSCLC. between CRP also...
Epstein-Barr virus (EBV) infection has been observed in tumor-infiltrated macrophages, but its effects on macrophage immune functions are poorly understood. Here, we showed that some macrophages the tumor stroma of nasopharyngeal carcinoma (NPC) tissue expressed immunosuppressive protein indoleamine 2,3-dioxygenase (IDO) more strongly than did cells. EBV induced mRNA, protein, and enzymatic activity IDO human monocyte-derived (MDMs). Infection increased production necrosis factor alpha...
Tumor-derived extracellular vesicles (TEVs) have emerged as promising sources of diagnostic and prognostic biomarkers for nasopharyngeal carcinoma (NPC). However, the lack high-sensitivity analytic methods ultratrace membrane proteins on TEVs hamper their clinical application TEVs. Herein, by combining aptamers that specifically bind to protein targets TEVs, PCR-based exponential amplification CRISPR/Cas12a real-time DNA detection, we developed a novel technique, termed aptamer-CRISPR/Cas12a...
Background: Currently, there are no molecular biomarkers for the early detection of non-small-cell lung cancer (NSCLC). This study focused on identifying RNAs found tumor-educated blood platelets (TEPs) detecting stage I NSCLC. Methods: Platelet RNAs, isolated from 9 patients with NSCLC (stages and II) 8 healthy controls, were analyzed using RNA-seq. ITGA2B was selected as a candidate marker. Two different Polymerase Chain Reactions (PCR) used to measure in platelet samples controls (n =...
Fusobacterium nucleatum (Fn) is a critical colorectal cancer (CRC)-associated bacterium. DNA hunger/stationary phase protective proteins (Dps) are bacterial ferritins that protect from oxidative stress. However, little known about the regulatory roles of Fn-Dps towards host cellular functions. Here, we identified culture supernatant Fn by mass spectrometry, and prepared recombinant protein. We show novel virulence protein Fn, Fn-Dps, which lyses disrupts erythrocytes competition for iron...
Abstract Differential diagnosis of pulmonary nodules detected by computed tomography (CT) remains a challenge in clinical practice. Here, we characterize the global metabolomes 480 serum samples including healthy controls, benign nodules, and stage I lung adenocarcinoma. The adenocarcinoma demonstrates distinct metabolomic signature, whereas controls share major similarities profiles. A panel 27 metabolites is identified discovery cohort ( n = 306) to distinguish between malignant nodules....
To evaluate whether serum Cathepsin S (Cat S) could serve as a biomarker for the diagnosis and prognosis of gastric cancer (GC), Enzyme-linked immuno sorbent assay (ELISA) was used to detect Cat in 496 participants including healthy controls patients with benign diseases, cancer, esophageal liver colorectal nasopharyngeal lung cancer. The levels were significantly increased patients, especially GC patients. qRT-PCR, Western blotting, immunohistochemical staining revealed overexpression cell...
The appearance and magnitude of the immune response related factors correlated with SARS-CoV-2 vaccination need to be defined. Here, we enrolled a prospective cohort 52 participants who received two doses inactivated vaccines (BBIBP-CorV). Their serial plasma samples (n = 260) over 2 months were collected at five timepoints. We measured antibody responses (NAb, S-IgG S-IgM) routine blood parameter. NAb seroconversion occurred in 90.7% vaccinated individuals four typical kinetic curves...
Abstract Programmed cell death receptor 1 (PD‐L1) protein on exosomes (exosomal PD‐L1) is one of the most promising biomarkers for cancer immunotherapy monitoring. However, current approaches exosomal PD‐L1 detection are poorly sensitive, laborious, and time‐consuming. Here, a new method, named Aptamer‐RPA‐TMA‐Cas13a Assay (ARTCA) established, which enables to be detected directly in serum with lower limit 10 particles mL −1 . Mechanistically, using DNA aptamer specifically binding PD‐L1,...
While growing evidence highlights the role of gut microbiota and inflammatory proteins in cancer, with cancer-related inflammation now considered seventh hallmark direct causal relationships between specific microbiota, potential mediating effects have not been fully established. We employed Mendelian randomization (MR) to assess proteins, eighteen distinct cancers using data from extensive genome-wide association studies (GWAS). The primary statistical method utilized was inverse variance...
The indoleamine 2,3-dioxygenase-(IDO-) mediated microenvironment plays an important role in tumor immune escape. However, the inhibitory effects of IDO on CD8(+) tumour-infiltrating lymphocytes (CD8(+) TILs) esophageal squamous cell carcinoma (ESCC) have not been clarified yet. Here, we found that level expression ESCC specimens correlated with a reduction number TILs. Patients high and low TILs had significantly impaired overall survival time. functional enzyme activity lines could be...