A5007256692- Cancer, Hypoxia, and Metabolism
- Catalytic C–H Functionalization Methods
- Acute Myeloid Leukemia Research
- Histone Deacetylase Inhibitors Research
- Mitochondrial Function and Pathology
- Synthesis and Catalytic Reactions
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- ATP Synthase and ATPases Research
- Metabolomics and Mass Spectrometry Studies
- Sulfur-Based Synthesis Techniques
- Genomics, phytochemicals, and oxidative stress
- Epigenetics and DNA Methylation
- Protein Degradation and Inhibitors
- Glutathione Transferases and Polymorphisms
- MicroRNA in disease regulation
- Cancer, Lipids, and Metabolism
- Radical Photochemical Reactions
- Synthesis and biological activity
- RNA modifications and cancer
- Nanoplatforms for cancer theranostics
- RNA Interference and Gene Delivery
- Redox biology and oxidative stress
- Crystallography and molecular interactions
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
Sun Yat-sen University
2016-2025
Sun Yat-sen University Cancer Center
2016-2025
State Key Laboratory of Oncology in South China
2014-2024
Wenzhou Medical University
2016-2024
Lishui Central Hospital
2020-2023
Lishui University
2022
Zhejiang University
2020-2022
China Pharmaceutical University
2021
Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University
2016
The University of Texas MD Anderson Cancer Center
2001-2012
Cancer cells exhibit increased glycolysis for ATP production due, in part, to respiration injury (the Warburg effect). Because generation through is less efficient than mitochondrial respiration, how cancer with this metabolic disadvantage can survive the competition other and eventually develop drug resistance a long-standing paradox. We report that defects lead activation of Akt survival pathway novel mechanism mediated by NADH. Respiration-deficient (ρ-) harboring DNA deletion dependency...
Abstract Generation of reactive oxygen species (ROS) has been observed in cancer cells treated with paclitaxel, but the underlying mechanisms and therapeutic implications remain unclear. In present study, we showed that paclitaxel promoted ROS generation through enhancing activity NADPH oxidase (NOX) associated plasma membranes. Treatment breast caused an increased translocation Rac1, a positive regulatory protein NOX, to membrane fraction. The paclitaxel-induced occurred rapidly within...
Superoxide dismutases (SODs) are important antioxidant enzymes responsible for the elimination of superoxide radical (O(2)(-)). The manganese-containing SOD (Mn-SOD) has been suggested to have tumor suppressor function and is located in mitochondria where majority O(2)(-) generated during respiration. Although increased reactive oxygen species (ROS) cancer cells long recognized, expression Mn-SOD its role development remain elusive. present study used a human tissue microarray analyze...
Although mitochondrial dysfunction and reactive oxygen species (ROS) stress have long been observed in cancer cells, their role promoting malignant cell behavior remains unclear. Here, we show that perturbation of the respiratory chain breast cells leads to a generation subclones with increased ROS, active proliferation, high cellular motility, invasive behaviors vitro vivo. Gene expression analysis using microarrays revealed all overexpressed CXCL14, novel chemokine undefined function. We...
To evaluate the safety and efficacy of combination histone deacetylase inhibitor vorinostat with idarubicin ara-C (cytarabine) in patients acute myelogenous leukemia (AML) or myelodysplastic syndrome (MDS).Patients previously untreated AML higher-risk MDS age 15 to 65 years appropriate organ function no core-binding factor abnormality were candidates. Induction therapy was 500 mg orally three times a day (days 1 3), idarubin 12 mg/m(2) intravenously (IV) daily × 3 4 6), cytarabine 1.5 g/m(2)...
Elevated aerobic glycolysis in cancer cells (the Warburg effect) may be attributed to respiration injury or mitochondrial dysfunction, but the underlying mechanisms and therapeutic significance remain elusive. Here we report that induction of respiratory defect by tetracycline-controlled expression a dominant negative form DNA polymerase γ causes metabolic shift from oxidative phosphorylation increases ROS generation. We show upregulation NOX is critical support elevated providing additional...
Abstract Reactive oxygen species (ROS) have a crucial role in cell signaling and cellular functions. Mounting evidences suggest that abnormal increase of ROS is often observed cancer cells this biochemical feature can be exploited for selective killing the malignant cells. A naturally occurring compound phenethyl isothiocyanate (PEITC) has been shown to promising anticancer activity by modulating intracellular ROS. Here we report novel synthetic analog PEITC with superior vitro vivo...
// Peng Wang 1,2,* , Chao-feng Zhu 1,* Ming-zhe Ma Gang Chen 3,* Ming Song 1 Zhao-lei Zeng Wen-hua Lu Jing Yang Shijun Wen Paul J. Chiao 4 Yumin Hu and Huang 1,3 Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center Medicine, Guangzhou, China 2 Department Emergency Memorial Hospital, 3 Translational Molecular Pathology, The Texas MD Anderson Houston, TX, USA Cellular Oncology, * These authors contributed equally to this work...
Linear iodoniums are widely used as arylating reagents. However, cyclic diaryl idodoniums ignored despite their potential to initiate dual arylations, atom and step economically. In our current work, a three-component cascade reaction of diaryliodoniums, sodium azide, alkynes has been successfully achieved under mild conditions, catalyzed by cheap copper species. The regioselectivity associated with unsymmetrical was enhanced installing two methyls ortho para the I(III) center. enables rapid...
Abstract Cancer side-population (SP) represents a sub-population of stem-like cancer cells that have an important role in drug resistance due to their high expression the ATP-binding cassette transporter ABCG2 involved export. Auranofin (AF), clinical gold complex is used treatment rheumatoid arthritis, has been reported inducing tumor antiproliferation. However, whether AF can impact SP remains unclear. Our study showed caused depletion and downregulation stem cell markers, impaired ability...
Cancer stem cells (CSCs) are thought to play an important role in tumor recurrence and drug resistance, present a major challenge cancer therapy. The microenvironment such as growth factors, nutrients oxygen affect CSC generation proliferation by providing the necessary energy sources signals. side population (SP) analysis has been used detect stem-like cell populations based on their high expression of ABCG2 that exports Hoechst-33342 certain cytotoxic drugs from cells. purpose this...
Traditional EZH2 inhibitors are developed to suppress the enzymatic methylation activity, and they may have therapeutic limitations due nonenzymatic functions of in cancer development. Here, we report proteolysis-target chimera (PROTAC)-based degraders target whole lymphoma. Two series were designed synthesized hijack E3 ligase systems containing either von Hippel–Lindau (VHL) or cereblon (CRBN), some VHL-based compounds able mediate degradation. best degraders, YM181 YM281, induced robust...
Patients with peritoneal metastasis (PM) of colorectal cancer (CRC) have poorer overall survival outcomes than those without PM. Cancer-associated fibroblasts (CAFs) are a major component the tumor microenvironment and mediate CRC progression It is imperative to identify develop novel therapeutic targets for PM-CRC driven by CAFs. Using lipidomics, we reveal that abundance phosphatidylcholine (PC) unsaturated acyl chains was increased in clinical specimens. Additionally, found CAFs were...
Untargeted metabolomic analysis using mass spectrometry provides comprehensive metabolic profiling, but its medical application faces challenges of complex data processing, high inter-batch variability, and unidentified metabolites. Here, we present DeepMSProfiler, an explainable deep-learning-based method, enabling end-to-end on raw signals with output accuracy reliability. Using cross-hospital 859 human serum samples from lung adenocarcinoma, benign nodules, healthy individuals,...
Abstract Mutations in isocitrate dehydrogenases (IDH) are oncogenic events due to the generation of metabolite 2-hydroxyglutarate. However, role wild-type IDH cancer development remains elusive. Here we show that IDH2 is highly expressed triple negative breast (TNBC) cells and promotes their proliferation vitro tumor growth vivo. Genetic silencing or pharmacological inhibition wt-IDH2 causes a significant increase α-ketoglutarate (α-KG), indicating suppression reductive tricarboxylic acid...
The slow growth of cells in the inner core solid tumors presents a form multidrug resistance to most standard chemotherapeutic agents, which target outer more rapidly dividing cells. However, anaerobic environment centrally located tumor also provides an opportunity exploit their dependence on glycolysis for therapeutic gain. We have developed two vitro models investigate this possibility. Model A represents osteosarcoma wild-type (wt) treated with agents inhibit mitochondrial oxidative...
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) plays an important role in multiple cellular functions including metabolism and gene transcription. Our previous study showed that GAPDH expression was elevated colon cancer further upregulated liver metastatic tissues, suggesting a possilbe of promoting metastasis. The present designed to investigate the underlying mechanism, using experimental approaches genetic silencing by short hairpin RNA (shRNA) biochemcial/molecular analyses key events...
K-ras mutations are major genetic events that drive cancer development associated with aggressive malignant phenotypes, while expression of the immune checkpoint molecule PD-L1 plays a key role in evasion surveillance also profoundly affects patient outcome. However, relationship between oncogenic signal and expressions as an important area requires further investigation. Using both vitro vivo experimental models K-ras-driven cancer, we found significantly enhanced through redox-mediated...
Abstract Background Cancer cells primarily utilize aerobic glycolysis for energy production, a phenomenon known as the Warburg effect. Increased supports cancer cell survival and rapid proliferation predicts poor prognosis in patients. Methods Molecular profiles from The Genome Atlas (TCGA) cohort were used to analyze prognostic value of gene signature human cancers. Gain- loss-of-function studies performed key drivers implicated hepatocellular carcinoma (HCC) glycolysis. molecular...
Abstract Differential diagnosis of pulmonary nodules detected by computed tomography (CT) remains a challenge in clinical practice. Here, we characterize the global metabolomes 480 serum samples including healthy controls, benign nodules, and stage I lung adenocarcinoma. The adenocarcinoma demonstrates distinct metabolomic signature, whereas controls share major similarities profiles. A panel 27 metabolites is identified discovery cohort ( n = 306) to distinguish between malignant nodules....