A5100326082- Gut microbiota and health
- Clostridium difficile and Clostridium perfringens research
- Extracellular vesicles in disease
- Advanced biosensing and bioanalysis techniques
- Streptococcal Infections and Treatments
- Immune cells in cancer
- Colorectal Cancer Screening and Detection
- Microscopic Colitis
- Plant-based Medicinal Research
- RNA modifications and cancer
- Cancer-related gene regulation
- Nanowire Synthesis and Applications
- Liver Disease Diagnosis and Treatment
- Cancer Research and Treatments
- Tryptophan and brain disorders
- Mycobacterium research and diagnosis
- Epigenetics and DNA Methylation
- Nosocomial Infections in ICU
- Gastrointestinal disorders and treatments
- RNA Interference and Gene Delivery
- Hepatitis B Virus Studies
- MicroRNA in disease regulation
- Metabolomics and Mass Spectrometry Studies
- Semiconductor materials and devices
- Diphtheria, Corynebacterium, and Tetanus
Sun Yat-sen University
2016-2024
Sun Yat-sen University Cancer Center
2022-2024
State Key Laboratory of Oncology in South China
2022
Inner Mongolia Autonomous Region Hospital of Traditional Chinese Medicine
2012
Objective Exosomes released from tumour cells are packed with unique RNA and protein cargo, they emerging as an important mediator in the communication network that promotes progression. The facultative intracellular bacterium Fusobacterium nucleatum (Fn) is colorectal cancer (CRC)-associated bacterium. To date, function of exosomes Fn-infected CRC has not been explored. Design were isolated by sequential differential centrifugation verified transmission electron microscopy, NanoSight...
Gut microbial dysbiosis contributes to the development of colorectal cancer (CRC). We evaluated utility fecal bacterial biomarker candidates identified by our 16S rDNA sequencing analysis for CRC diagnosis.We measured relative abundance Fusobacterium nucleatum (Fn), Faecalibacterium prausnitzii (Fp), Bifidobacterium (Bb), and Lactobacillus (Lb) quantitative PCR in samples from 2 cohorts 903 individuals. validated diagnostic performance these ratios investigated antagonistic effect Fn against...
Fusobacterium nucleatum (F. nucleatum, Fn) is associated with the colorectal cancer (CRC). Fn-infection could induce significant levels of serum Fn-specific antibodies in human and mice. The objective this study was to identify that elicit a humoral response patients CRC evaluate diagnostic performance anti-Fn antibodies. In work, we showed mean absorbance value anti-Fn-IgA -IgG group were significantly higher than those benign colon disease healthy control (P < 0.001). sensitivity...
The tumor-associated microbiota plays a vital role in cancer development. Accumulating evidence shows that Fusobacterium nucleatum (Fn) participates the progression of multiple tumor types. However, underlying mechanisms remain unclear. This study examined expression methyltransferase-like protein 3 (METTL3) during Fn infection and elucidated function pathway Fn-induced m6A methylation esophageal squamous cell carcinoma (ESCC). abundance patient tissues was determined by qPCR. Western blot,...
Abstract Fusobacterium nucleatum ( Fn ) is a tumor-associated obligate anaerobic bacterium, which has role in the progression of colorectal cancer (CRC). can invade and promote colon epithelial cells proliferation. However, how survives proliferates its host remains largely unknown. In this study, we aimed to determine molecular mechanisms underlying morphology, survival, proliferation THP-1-derived macrophages (dTHP1). For first time, found that facultative intracellular bacterium survive...
Tumor-derived extracellular vesicles (TEVs) have emerged as promising sources of diagnostic and prognostic biomarkers for nasopharyngeal carcinoma (NPC). However, the lack high-sensitivity analytic methods ultratrace membrane proteins on TEVs hamper their clinical application TEVs. Herein, by combining aptamers that specifically bind to protein targets TEVs, PCR-based exponential amplification CRISPR/Cas12a real-time DNA detection, we developed a novel technique, termed aptamer-CRISPR/Cas12a...
Fusobacterium nucleatum (Fn) is a critical colorectal cancer (CRC)-associated bacterium. DNA hunger/stationary phase protective proteins (Dps) are bacterial ferritins that protect from oxidative stress. However, little known about the regulatory roles of Fn-Dps towards host cellular functions. Here, we identified culture supernatant Fn by mass spectrometry, and prepared recombinant protein. We show novel virulence protein Fn, Fn-Dps, which lyses disrupts erythrocytes competition for iron...
Abstract Programmed cell death receptor 1 (PD‐L1) protein on exosomes (exosomal PD‐L1) is one of the most promising biomarkers for cancer immunotherapy monitoring. However, current approaches exosomal PD‐L1 detection are poorly sensitive, laborious, and time‐consuming. Here, a new method, named Aptamer‐RPA‐TMA‐Cas13a Assay (ARTCA) established, which enables to be detected directly in serum with lower limit 10 particles mL −1 . Mechanistically, using DNA aptamer specifically binding PD‐L1,...
Methyltransferase-like 3 (METTL3) is an RNA methyltransferase mediating N6 methyladenosine (m6A) modification. Its role in cancer pathogenesis and progression has attracted increasing attention. However, the immunological role, possible immune mechanism, clinical significance of METTL3 esophageal squamous cell carcinoma (ESCC) remain to be confirmed. The Tumor Genome Atlas (TCGA) provided transcriptome sequencing data for this study (162 tumor tissue samples 11 normal samples), while...
The intracellular bacterium Fusobacterium nucleatum (Fn) mediates tumorigenesis and progression in colorectal cancer (CRC). However, the origin of intratumoral Fn role Fn-infected immunocytes tumor microenvironment remain unclear. Here, we observed that neutrophils/macrophages (PMNs/MΦs), especially PMNs, accumulate tissues fecal abundance correlates positively with an blood PD-L1+ PMNs CRC patients. Moreover, accumulates tumor-bearing mice via intragingival infection intravenous injection....
Fusobacterium nucleatum (Fn) is an important tumour-associated bacterium in colorectal cancer (CRC). The antioxidant protein alkyl hydroperoxide reductase subunit C (AhpC) can induce strong antibacterial immune response during various pathogen infections. Our study aimed to evaluate the efficacy of Fn-AhpC as a candidate vaccine. In this work, by western blot analysis, we showed that recombinant could be recognized specifically antibodies present sera CRC patients; using mouse Fn-infection...
Background and Aims: Intestinal dysbiosis is implicated in the pathogenesis of Crohn's disease (CD). We evaluated fecal sera microbial markers for clinical use detecting CD. Methods: Fecal samples from 346 Asian subjects were collected, including 95 patients with CD, 81 ulcerative colitis (UC), 65 irritable bowel syndrome (IBS), 105 healthy (HS). Microbial indicators Fusobacterium nucleatum (Fn), Faecalibacterium prausnitzii (Fp) Escherichia coli (E. coli) identified based on a review...
Programmed cell death receptor 1 (PD-L1) protein on exosomes (exosomal PD-L1) is one of the most promising biomarkers for cancer immunotherapy monitoring. However, current approaches exosomal PD-L1 detection are poorly sensitive, laborious, and time-consuming. Here, a new method, named Aptamer-RPA-TMA-Cas13a Assay (ARTCA) established, which enables to be detected directly in serum with lower limit 10 particles mL−1. Mechanistically, using DNA aptamer specifically binding PD-L1, amplified...
Background: Fusobacterium nucleatum (Fn) is a critical colorectal cancer (CRC)-associated bacterium. DNA hunger/stationary phase protective proteins (Dps) are bacterial ferritins that protect from oxidative stress. However, little known regarding the host cell responses to Dps.Methods: Fn-Dps was identified culture supernatant of Fn by mass spectrometry. The pET28a-Fn-Dps plasmid constructed prepare recombinant protein. In vitro effect on erythrocytes determined count with hemocytometer....