A5101592136- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- RNA modifications and cancer
- Circular RNAs in diseases
- interferon and immune responses
- Kruppel-like factors research
- FOXO transcription factor regulation
- Extracellular vesicles in disease
- RNA Research and Splicing
- Zebrafish Biomedical Research Applications
- RNA and protein synthesis mechanisms
- Helicobacter pylori-related gastroenterology studies
- Receptor Mechanisms and Signaling
- Epigenetics and DNA Methylation
- Cancer, Hypoxia, and Metabolism
- RNA Interference and Gene Delivery
- Infant Nutrition and Health
- Ferroptosis and cancer prognosis
- PI3K/AKT/mTOR signaling in cancer
- Cancer Cells and Metastasis
- Cancer-related gene regulation
- Neuropeptides and Animal Physiology
- Advanced biosensing and bioanalysis techniques
- Cancer, Lipids, and Metabolism
- Neurotransmitter Receptor Influence on Behavior
Guangzhou Medical University
2013-2025
Guangzhou Medical University Cancer Hospital
2014-2025
Sun Yat-sen University
2013-2023
Sun Yat-sen University Cancer Center
2013-2023
University Gastroenterology
2019
Soochow University
2014-2018
Shanghai Institutes for Biological Sciences
2009-2014
Chinese Academy of Sciences
2009-2014
Ruijin Hospital
2009-2014
Shanghai Jiao Tong University
2009-2014
It has been reported that increasingly microRNAs are associated with diseases. However, the patterns among microRNA-disease associations remain largely unclear. In this study, in order to dissect of associations, we performed a comprehensive analysis human association data, which is manually collected from publications. We built microRNA disease network. Interestingly, tend show similar or different dysfunctional evidences for clusters, respectively. A negative correlation between...
N4-acetylcytidine (ac4C), a widespread modification in human mRNAs that is catalyzed by the N-acetyltransferase 10 (NAT10) enzyme, plays an important role promoting mRNA stability and translation. However, biological functions regulatory mechanisms of NAT10-mediated ac4C were poorly defined.ac4C status NAT10 expression levels analyzed gastric cancer (GC) samples compared with corresponding normal tissues. The its upstream downstream determined vitro vivo. therapeutic potential targeting GC...
The purpose of this study was to investigate the clinicopathologic significance and potential role miR-200b miR-200c in development progression gastric cancer.We examined expression 36 paired normal stomach tumor specimens, as well cancer cell lines, by quantitative real-time PCR. In addition, were detected ISH using tissue microarrays, association between levels factors prognosis analyzed. A luciferase assay conducted for target evaluation. functional effects on cells validated a...
MicroRNAs (miRNAs) are small noncoding RNAs that involved in various diseases, including cancer. In the present study, we found miR-216b was downregulated nasopharyngeal carcinoma (NPC) cell lines and specimens. Decreased expression of directly related to advanced clinical stage lymph node metastasis. levels correlated inversely with KRAS protein during tumorigenesis. Furthermore, demonstrated can bind 3′ untranslated region (UTR) inhibit protein. Both vitro vivo assays revealed attenuated...
MicroRNAs have been considered as a kind of potential novel biomarker for cancer detection due to their remarkable stability in the blood and characteristics expression profile many diseases.We performed microarray-based serum miRNA profiling on twenty nasopharyngeal carcinoma patients at diagnosis along with 20 non-cancerous individuals controls. This was followed by real-time quantitative Polymerase Chain Reaction (RT-qPCR) separate cohort thirty age- matched volunteers. A model...
Breast cancer stem cells (BCSCs) are tumor initiating that can self-renew and highly tumorigenic chemoresistant. Therefore, the identification of factors critical for BCSC function is vital development therapies. Here, we report DNMT1-mediated FOXO3a promoter hypermethylation leads to downregulation expression in breast cancer. functionally related inhibition FOXM1/SOX2 signaling consequent suppression BCSCs properties tumorigenicity. Moreover, found SOX2 directly transactivates DNMT1...
MicroRNAs (miRNAs) show considerable promise as therapeutic agents to improve tumor treatment, they have been revealed crucial modulators in progression. However, our understanding of their roles gastric carcinoma (GC) metastasis is limited. Here, we aimed identify novel miRNAs involved GC and explored regulatory mechanisms significance GC.
LTF (lactotransferrin, or lactoferrin) plays important role in innate immunity, and its anti-tumor function has also been reported multiple cancers. We previously that is significantly down-regulated nasopharyngeal carcinoma (NPC) acts as a tumor suppressor by suppressing AKT signaling. However, the exact mechanism of down-regulation NPC not revealed. In current study, we screened identified bona fide target miR-214 cells. mimics suppressed mRNA protein expression levels only can promote...
Abstract Increasing evidence suggest that lncRNAs (long noncoding RNAs) play important roles in human cancer. Breast cancer is a heterogeneous disease and the potential involvement of breast remains unexplored. In this study, we characterized novel lncRNA, RP1-5O6.5 (termed as RP1 ). We found was highly expressed predicted poor prognosis patients. Gain-of-function loss-of-function assays showed promoted proliferation metastasis cells vitro vivo. Mechanistically, maintained EMT stemness...
Tumor-derived extracellular vesicles (TEVs) have emerged as promising sources of diagnostic and prognostic biomarkers for nasopharyngeal carcinoma (NPC). However, the lack high-sensitivity analytic methods ultratrace membrane proteins on TEVs hamper their clinical application TEVs. Herein, by combining aptamers that specifically bind to protein targets TEVs, PCR-based exponential amplification CRISPR/Cas12a real-time DNA detection, we developed a novel technique, termed aptamer-CRISPR/Cas12a...
miRNAs have been proposed to be key regulators of progression and metastasis in cancer. However, an understanding their roles molecular mechanisms is needed provide deeper insights for better therapeutic opportunities. In this study we investigated the role mechanism miR-493 development nonsmall-cell lung cancer (NSCLC). Our data indicated that expression was markedly reduced pulmonary carcinoma. The ectopic impaired cell growth invasion vitro vivo. Mechanically, commonly directly targeted...
Background Reduced expression of developmentally important genes and tumor suppressors due to haploinsufficiency or epigenetic suppression has been shown contribute the pathogenesis various malignancies. However, methodology that allows spatio-temporally knockdown gene in model organisms such as zebrafish not well established, which largely limits potential a vertebrate human malignant disorders. Principal Finding Here, we report multiple copies small hairpin RNA (shRNA) are expressed from...
// Xiaoting Jia 1, * , Nan Li 2, Cong Peng 1 Yingen Deng Wang 2 Min Minying Lu Jiang Yin Guopei Zheng Haiying Liu Zhimin He Cancer Research Institute and Center of Guangzhou Medical University, Key Laboratory “Translational Medicine on Malignant Tumor Treatment”, 510095, Guangdong, China Department Gastrointestinal Neoplasms Surgery, These authors contributed equally to this work Correspondence to: Zheng, e-mail: zhengguopei@126.com He, hezhimin2005@yahoo.com Keywords: miR-493,...
MicroRNAs (miRNAs) can regulate the post-transcriptional level of gene expression. It has been documented that downregulation miR-206 is significant in human gastric cancer (GC), whereas its role GC cell biological behaviors, including proliferation, migration, and invasion, not thoroughly investigated. MiR-206 levels have a negative association with lymph node metastasis tumor patients higher expression better prognoses. Functional studies demonstrated overexpression significantly...
Abstract Sorafenib, which is proven to serve as a potent ferroptosis inducer, used first‐line treatment for patients with advanced hepatocellular carcinoma (HCC), but it has limited clinical benefits, mainly due drug resistance. Herein, using genome‐wide CRISPR/Cas9 knockout screening and multiple functional studies, this work identifies COP9 signalosome subunit 5 (COPS5) driver of sorafenib resistance suppressor in HCC. Consistently, the amplification overexpression COPS5 are frequently...
Although thousands of long noncoding RNAs (lncRNAs) have been annotated, only a few lncRNAs characterized functionally. In this study, we aimed to identify novel involved in the progression gastric carcinoma (GC) and explore their regulatory mechanisms clinical significance GC.A lncRNA expression microarray was used differential profiles between paired GCs adjacent normal mucosal tissues. Using above method, SGO1-AS1 selected for further study. Quantitative reverse transcription polymerase...
miR-122 plays a vital role in the development of chronic hepatitis B virus (HBV) infection and hepatocellular carcinoma (HCC). Based on data from Encyclopedia DNA Elements (ENCODE), two single nucleotide polymorphisms (SNPs), rs4309483 rs4503880, were identified upstream regulatory region miR-122. A case–control study consisting 1,300 HBV-positive HCC cases, 1,344 HBV carriers, persons who cleared naturally was carried out to test association between SNPs risk for HCC. The CA/AA genotypes...
Abstract MiRNA isoforms (isomiRs) were defined as an addition or deletion of one more nucleotides at the 5′ 3′ ends both. Different isomiRs same miRNA can target different genes, which have extended regulatory scale medicated by miRNA. In this study, we systematically analyzed in hepatocellular carcinoma (HCC) based on The Cancer Genome Atlas (TCGA) data and further explore their role silico vitro studies. We found that higher variety quantity miR‐139‐5p negatively correlated with malignancy...