A5100321797- Advanced biosensing and bioanalysis techniques
- Extracellular vesicles in disease
- Gold and Silver Nanoparticles Synthesis and Applications
- RNA Interference and Gene Delivery
- Biosensors and Analytical Detection
- Advanced Nanomaterials in Catalysis
- Gas Sensing Nanomaterials and Sensors
- Nanowire Synthesis and Applications
- Glutathione Transferases and Polymorphisms
- Mass Spectrometry Techniques and Applications
- Sexual Differentiation and Disorders
- Cell death mechanisms and regulation
- CRISPR and Genetic Engineering
- Advanced Proteomics Techniques and Applications
- Molecular Sensors and Ion Detection
- Urological Disorders and Treatments
- Genomics, phytochemicals, and oxidative stress
- Immune Cell Function and Interaction
- Cancer-related molecular mechanisms research
- SARS-CoV-2 detection and testing
- Luminescence and Fluorescent Materials
- Lung Cancer Treatments and Mutations
- Diffusion and Search Dynamics
- MicroRNA in disease regulation
- Circular RNAs in diseases
Shanghai Chest Hospital
2025
Shanghai Jiao Tong University
2025
Tianjin Medical University Cancer Institute and Hospital
2021-2023
Xihua University
2022-2023
Sun Yat-sen University
2020
Sun Yat-sen University Cancer Center
2020
Southwest Petroleum University
2019
Southwest University
2015-2019
Chongqing University
2019
Second Affiliated Hospital of Xinjiang Medical University
2017
Oxaliplatin resistance is a challenge in the treatment of colorectal cancer (CRC) patients. Regulatory T cells (Tregs) are well known for their immunosuppressive roles, and targeting Tregs an effective way to improve chemosensitivity. Exosome-delivered microRNA (miRNA) might be used as potential biomarker predicting However, relationship between exosomal miRNAs remains largely unknown. TaqMan low-density array was performed screen differentially expressed serum from pooled patients who had...
In this work, an "off" to "on" surface-enhanced Raman spectroscopy (SERS) platform was constructed for ultrasensitive detection of microRNA (miRNA) by using a magnetic SERS substrate (Co@C/PEI/Ag) and padlock probe-based exponential rolling circle amplification (P-ERCA) strategy. Herein, miRNA 155 could act as primers initiate (RCA) producing long repeat sequence, then the obtained DNA would be cleaved into two kinds single-stranded DNAs in presence nickase. One can new primer cycle...
N-terminal pro-brain natriuretic peptide (NT-proBNP) has been demonstrated to be a sensitive and specific biomarker for heart failure (HF). Surface-enhanced Raman spectroscopy (SERS) technology can used accurately detect NT-proBNP at an early stage its advantages of high sensitivity, less wastage time consumption. In this work, we have new SERS-based immunosensor ultrasensitive analysis by using metal-organic frameworks (MOFs)@Au tetrapods (AuTPs) immobilized toluidine blue as SERS tag....
Usually, SERS technology requires labeling of the Raman reporter to obtain characteristic spectra for detection biological samples. However, number labeled reporters is often limited, resulting in restricted improvement sensitivity biosensor. In this work, switchable target-responsive 3D DNA hydrogels were introduced precisely control trapping and release toluidine blue (TB), which not only avoid signal molecule but also improve miRNA due immobilization abundant TB. absence target miRNA,...
Tumor-derived extracellular vesicle (TEV) protein biomarkers facilitate cancer diagnosis and prognostic evaluations. However, the lack of reliable convenient quantitative methods for evaluating TEV proteins prevents their clinical application. Methods: Here, based on dual amplification hybridization chain reaction (HCR) CRISPR-Cas12a, we developed apta-HCR-CRISPR assay direct high-sensitivity detection proteins. The protein-targeted aptamer was amplified by HCR to produce a long-repeated...
In this work, we have demonstrated a novel electrochemical method based on target-induced cleavage of specific peptide for sensitive analysis prostate antigen (PSA) by using silver enhancement. First, multiwalled carbon nanotubes/poly(amidoamine) dendrimers (MWCNTs–PAMAM) nanohybrids were assembled the electrode to bind peptide. Subsequently, dithiobis(succinimidylpropionate) (DSP)@Au@SiO2 was prepared as tracing tag and covalent bond with peptides via inherent interaction between DSP amino...
Tumor-derived extracellular vesicles (TEVs) have emerged as promising sources of diagnostic and prognostic biomarkers for nasopharyngeal carcinoma (NPC). However, the lack high-sensitivity analytic methods ultratrace membrane proteins on TEVs hamper their clinical application TEVs. Herein, by combining aptamers that specifically bind to protein targets TEVs, PCR-based exponential amplification CRISPR/Cas12a real-time DNA detection, we developed a novel technique, termed aptamer-CRISPR/Cas12a...
Lymph node metastasis (LNM) is the prominent route of gastric cancer dissemination, and usually leads to tumor progression a dismal prognosis cancer. Although exosomal lncRNAs have been reported be involved in development, whether secreted can encode peptides recipient cells remains unknown. Here, we identified an lncRNA (lncAKR1C2) that was clinically correlated with lymph VEGFC-independent manner. Exo-lncAKR1C2 from demonstrated enhance tube formation migration lymphatic endothelial cells,...
In this work, one stimuli-responsive DNA microcapsule was designed to combine duplex-specific nuclease (DSN) amplification strategy and surface-enhanced Raman spectroscopy (SERS) technology for sensitive detection of microRNA 155 (miRNA 155). First, toluidine blue (TB) as dye CaCO3 core templates co-precipitated form TB@CaCO3 composite. Then, networks were layer by constructed with oligonucleotide layers cross-linked linker ssDNA L lock inside. the presence ethylenediaminetetraacetic acid,...
A ratiometric SERS biosensor with DNA hydrogel-captured glucose oxidase amplification method was fabricated to detect microRNA 122.
Abstract OBJECTIVES Intensive care unit (ICU) readmission has been proposed as a metric for quality of surgical care. The current study investigated potential factors and developed prediction model ICU in patients following oesophagectomy cancer. METHODS A total 3028 from 2019.1 to 2022.12 were retrospectively collated training cohort, with 829 2023.1 2023.8 enrolled validation, respectively. Univariable multivariable analysis performed identify after which nomogram based on results was...
Abstract Programmed cell death receptor 1 (PD‐L1) protein on exosomes (exosomal PD‐L1) is one of the most promising biomarkers for cancer immunotherapy monitoring. However, current approaches exosomal PD‐L1 detection are poorly sensitive, laborious, and time‐consuming. Here, a new method, named Aptamer‐RPA‐TMA‐Cas13a Assay (ARTCA) established, which enables to be detected directly in serum with lower limit 10 particles mL −1 . Mechanistically, using DNA aptamer specifically binding PD‐L1,...
Intake of water contaminated with mercury ions leads to various toxic effects and health issues. In this work, a novel gold nanoparticles (AuNPs) DNA hybrid sensor for fluorescent detection mercury(II) (Hg2+) in aqueous solution was prepared. The fluorescein modified onto the terminal ssDNA it consequently presented strong fluorescence. probing behaviors toward metal were investigated through fluorescence intensity change. result demonstrated that amplification strategy based on ssDNA−AuNPs...
In this work, we adopted polyacrylamide gel-contained zinc finger peptide (PZF) as a "lock" of Raman signal and ions (Zn2+) sensitive "key", which was converted from target-captured ZnO NPs, to achieve the measurement prostate-specific antigen (PSA). Owing lock effect PZF, surface-enhanced scattering (SERS) tag toluidine blue (TB) connected on Ag NP-coating silica wafer sheltered leading low response. Meanwhile, target PSA can specifically connect with antibody 2-coupled nanocomplexes...
Background: Previous studies showed that genetic polymorphisms of glutathione S-transferase P1 (GSTP1) were involved in metabolism and ribonucleotide reductase (RRM1) correlated with DNA synthesis. Here we explored the effects these on chemosensitivity clinical outcome Chinese non-small cell lung cancer (NSCLC) patients treated gemcitabine-cisplatin regimens. Materials Methods: sequencing was used to evaluate GSTP1 Ile105Val RRM1 C37A-T524C 47 NSCLC Clinical response evaluated according...
The digit ratio has been used as a retrospective noninvasive biomarker to investigate the putative effects of prenatal exposure androgens. In recent years, many scholars have paid attention association between 2D:4D (the second and fourth digits) prostatic cancer. This study explored prognostic significance in prostate cancer patients. We reviewed progressive status survival 382 patients who had received hormone therapy at our institutions. Survival clinicopathological variables analyzed...