A5100327375- Autophagy in Disease and Therapy
- Computational Drug Discovery Methods
- Calcium signaling and nucleotide metabolism
- Pharmacological Effects of Natural Compounds
- Endoplasmic Reticulum Stress and Disease
- Mosquito-borne diseases and control
- Pituitary Gland Disorders and Treatments
- Toxoplasma gondii Research Studies
- Congenital Heart Disease Studies
- Cell death mechanisms and regulation
- Immune cells in cancer
- Aquaculture disease management and microbiota
- Ophthalmology and Eye Disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Ginseng Biological Effects and Applications
- Sirtuins and Resveratrol in Medicine
- Ubiquitin and proteasome pathways
- DNA Repair Mechanisms
- Glioma Diagnosis and Treatment
- Adrenal and Paraganglionic Tumors
- Cerebral Venous Sinus Thrombosis
- Extracellular vesicles in disease
- Traditional Chinese Medicine Analysis
- Ergonomics and Musculoskeletal Disorders
- Curcumin's Biomedical Applications
Nanjing Drum Tower Hospital
2025
Zhejiang Chinese Medical University
2017-2025
Shuguang Hospital
2024
Shanghai University of Traditional Chinese Medicine
2024
State Key Laboratory of Information Engineering in Surveying Mapping and Remote Sensing
2024
Union Hospital
2024
Huazhong University of Science and Technology
2022-2024
First Hospital of China Medical University
2024
China Medical University
2024
Guangzhou University of Chinese Medicine
2024
Abstract The steady flow of lactic acid (LA) from tumor cells to the extracellular space via monocarboxylate transporter symport system suppresses antitumor T cell immunity. However, LA is a natural energy metabolite that can be oxidized in mitochondria and could potentially stimulate cells. Here we show lactate-lowering mood stabilizer lithium carbonate (LC) inhibit LA-mediated CD8 + immunosuppression. Cytoplasmic increased pumping protons into lysosomes. LC interfered with vacuolar ATPase...
There is an urgent need for new therapeutic strategies patients with glioblastoma multiforme (GBM). Previous studies have shown that berberine (BBR), a natural plant alkaloid, has potent anti-tumor activity. However, the mechanisms leading to cancer cell death not been clearly elucidated. In this study, we show BBR profound effects on metabolic state of GBM cells, high autophagy flux and impaired glycolytic capacity. Functionally, these alterations reduce invasive properties, proliferative...
Objective Autophagy participates in the progression of hepatocellular carcinoma (HCC) and resistance HCC cells to sorafenib. We investigated feasibility sensitising sorafenib by modulating miR-541-initiated microRNA-autophagy axis. Design Gain- loss-of-function assays were performed evaluate effects miR-541 on malignant properties autophagy human cells. was quantified western blotting LC3, transmission electron microscopy analyses confocal scanning mRFP-GFP-LC3 reporter construct. Luciferase...
The atherogenic index of plasma (AIP) can reflect the burden atherosclerosis. Hyperglycemia is one leading causes However, relationship between AIP and prediabetes rarely studied. Therefore, we aimed to explore prediabetes.This retrospective cohort study recruited 100,069 Chinese adults at Rich Healthcare Group from 2010 2016. was calculated according Log10 (triglyceride/high-density lipoprotein cholesterol) formula. Cox regression method, sensitivity analyses subgroup were used examine...
Autophagy, a cellular process involving lysosomal self-digestion, plays crucial role in recycling biomolecules and degrading dysfunctional proteins damaged organelles. However, non-small cell lung cancer (NSCLC), cells can exploit autophagy to survive metabolic stress develop resistance epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKIs), which reduce treatment efficacies. Currently, most studies have found that late-stage hinder EGFR-TKIs resistance, while research on...
A central task for a program analysis concerns how to efficiently find abstraction that keeps only information relevant proving properties of interest. We present new approach finding such abstractions analyses written in Datalog. Our is based on counterexample-guided refinement: when Datalog run fails using an abstraction, it seeks generalize the cause failure other abstractions, and pick avoids similar failure. solution uses boolean satisfiability formulation general, complete, optimal:...
Abstract Background Glioblastoma multiforme (GBM) is characterized by lethal aggressiveness and patients with GBM are in urgent need for new therapeutic avenues to improve quality of life. Current studies on tumor invasion focused roles cytokines microenvironment numerous evidence suggests that TGF-β2 abundant glioma vital invasion. Autopagy also emerging as a critical factor aggressive behaviors cancer cells; however, the relationship between autophagy has been poorly understood. Methods...
Lipid catabolism disorder is the primary cause of atherosclerosis. Transcription factor EB (TFEB) prevents atherosclerosis by activating macrophage autophagy to promote lipid degradation. Hypericin-mediated sonodynamic therapy (HY-SDT) has been proved non-invasively inducing THP-1-derived apoptosis; however, it unknown whether could be triggered HY-SDT influence cellular via regulating TFEB. Here, we report that resulted in time-dependent activation through AMPK/AKT/mTOR pathway. Besides,...
Reactive oxygen species (ROS) are emerging as important regulators of cancer growth and metastatic spread. However, how cells integrate redox signals to affect progression is not fully understood. Mitochondria cellular hubs, which highly regulated by interactions with neighboring organelles. Here, we investigated ROS at the endoplasmic reticulum (ER)-mitochondria interface generated translated melanoma outcome. We show that TMX1 TMX3 oxidoreductases, promote ER-mitochondria communication,...
Resistance to adjuvant radiotherapy is a major cause of treatment failure in patients with glioblastoma (GBM). Autophagy inhibitors have been shown enhance the efficacy for certain solid tumors. However, current do not penetrate blood-brain-barrier (BBB). Here, we assessed radiosensitivity effects antipsychotic drug trifluoperazine (TFP) on GBM vitro and vivo.U251 U87 cell lines as well cells from primary human biopsy (P3), were used vivo evaluate TFP treatment. Viability cytotoxicity was...
Abstract DNA damage can induce autophagy; however, the underlying mechanism remains largely unknown. Here we report that leads to autophagy through ATR/Chk1/RhoB-mediated lysosomal recruitment of TSC complex and subsequent mTORC1 inhibition. caused by ultraviolet light (UV) or alkylating agent methyl methanesulphonate (MMS) results in phosphorylation small GTPase RhoB Chk1. Phosphorylation enhances its interaction with TSC2, promotes sumoylation PIAS1, which is required for RhoB/TSC...
Autophagy is an important biological activity that maintains homeostasis in eukaryotic cells. However, little known about the functions of fish autophagy-related genes (Atgs). In this study, we cloned and characterized Atg5, a key gene autophagy superfamily, from orange-spotted grouper (Epinephelus coioides) (EcAtg5). EcAtg5 encoded 275-amino acid protein shared 94% 81% identity to seabass (Lates calcarifer) humans (Homo sapiens), respectively. The transcription level was significantly...
Background: Cerebral ischemia-reperfusion injury is caused by a blood reperfusion in the ischemic brain and usually occurs treatment stage of disease, which can aggravate tissue injury. Objective: Curcumin was reported to exert good therapeutic effect on neural cells against ischemia- injury, However, mechanism not clear. Methods: In this study, Oxygen-Glucose Deprivation (OGD) model fetal rat cerebral cortical neurons Middle Artery Occlusion (MCAO) rats were employed mimic vitro vivo,...
The existing treatments for ischemic stroke cannot meet the clinical needs so far. Quercetin (QT) is an effective apoptosis inhibitor and antioxidant flavonoid, but its water solubility poor has no targeting. In this study, QT modified with hyaluronic acid (HA) to form a water-soluble conjugate HA-QT, which can specifically bind CD44 receptors response hyaluronidase. Next, novel delivery system SS31-HA-QT prepared by further modification SS31, polypeptide capable of penetrating blood-brain...
Autophagy plays an important role in the infectious processes of diverse pathogens. For instance, cellular autophagy could be harnessed by viruses to facilitate replication. However, it is still uncertain about interplay and swine acute diarrhea syndrome coronavirus (SADS-CoV) cells. In this study, we reported that SADS-CoV infection induce a complete process both vitro vivo , inhibition significantly decreased production, thus suggesting facilitated replication SADS-CoV. We found ER stress...
Hepatocellular carcinoma (HCC) has a complex and changeable tumor microenvironment. Despite emerging evidence focusing on autophagy process within immune cells, the function regulatory mechanism of macrophage in progression remains unclear. Our results multiplex-immunohistochemistry RNA-sequencing identified reduced levels macrophages HCC microenvironment, associated with poor prognosis increased microvascular metastasis patients. Specifically, suppressed initiation through up-regulation...
AbstractThe ubiquitin-proteasome system (UPS) and the autophagy-lysosomal pathway (ALP) are two most important components of cellular mechanisms for protein degradation. In present study we investigated functional relationship systems interactional role p53 in vitro. Our showed that proteasome inhibitor lactacystin induced an increase level autophagy activity, whereas inhibition by pifithrin-α or small interference RNA (siRNA) attenuated induction increased aggregation. Furthermore, found...
To evaluate the anti-viral effect of emodin plus Astragalus polysaccharide (APS) in hepatitis B virus (HBV) transgenic mice.Sixty HBV mice (HBV TGM) whose weight varied between 18 and 24 g were randomly divided into 3 groups, with 20 each group. Group A was normal control, where treated physiological saline; group positive control lamivudine solution (100 mL/kg per day). C experimental saline containing APS (57.59 mg/kg day 287.95 day, respectively). The daily for wk. After 1 wk recovery...
Hepatitis B virus (HBV) is considered highly prevalent in West Africa. However, major gaps surveillance exist Sierra Leone. Although healthcare workers (HCWs) are at high risk for HBV infection, little known about the prevalence and knowledge of hepatitis among HCWs Leone.A cross-sectional study all No. 34 Military Hospital located Freetown, Leone, was conducted from March 20 to April 10, 2017. Whole blood collected screened markers using a one-step rapid immunochromatographic test with...