A5080758719- MicroRNA in disease regulation
- Advanced biosensing and bioanalysis techniques
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Molecular Biology Techniques and Applications
- RNA modifications and cancer
- RNA Research and Splicing
- Biosensors and Analytical Detection
- Cancer Genomics and Diagnostics
- Single-cell and spatial transcriptomics
- Gene expression and cancer classification
- Prenatal Screening and Diagnostics
- Extracellular vesicles in disease
- Microbial Community Ecology and Physiology
- RNA Interference and Gene Delivery
- Renal and related cancers
- Pregnancy and preeclampsia studies
- Advanced Biosensing Techniques and Applications
- Genomics and Phylogenetic Studies
- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- Ferroptosis and cancer prognosis
- DNA and Biological Computing
- RNA and protein synthesis mechanisms
- Nuclear Receptors and Signaling
Southeast University
2016-2025
State Key Laboratory of Digital Medical Engineering
2016-2025
BioElectronics (United States)
2019
Nanjing Library
2014
Ministry of Education
2011
Ministry of Education of the People's Republic of China
2007-2011
Exosomes are small membrane-bound vesicles secreted by most cell types. contain various functional proteins, mRNAs and microRNAs (miRNAs) that could be used for diagnostic therapeutic purposes. How we should store the samples before RNA isolation whether those long term stored circulating investigation because of RNase is unknown. The aim study was to determine stability miRNA in exosomes plasma. were isolated from plasma using ExoQuick Precipitation methods. extracted corresponding with a...
MicroRNAs (miRNAs) are a series of promising molecules that could regulate gene expression, while its expression level and type strictly related to the early diagnosis, targeted therapy, prognosis diseases. Improved detection accuracy miRNAs would lead unprecedented progress in treatment. Numerous methods improve sensitivity have been proposed confirmed valuable recently. However, research which especially targets at improving specificity remains rare. This Feature gives retrospective...
It is reported that different microRNA (miRNA) profiles can be detected in the blood of cancer patients. We investigated whether key serum miRNAs could discriminate patients with and without breast cancer. This study was divided into three parts: (1) miRNA marker discovery using SOLiD sequencing-based profiling on cancerous adjacent noncancerous tissue one patient; (2) selection validation by real-time PCR a small set serum; (3) gene ontology analysis target genes. Of genome-wide expression...
Preeclampsia (PE) is a pregnancy-specific syndrome and one of the leading causes maternal fetal morbidity mortality. The pathophysiological mechanisms PE remain poorly known. Recently, circulating miRNAs are considered as potential useful noninvasive biomarkers. aim this study was to identify differentially expressed plasma in preeclamptic pregnancies compared with normal pregnancies. Maternal miRNA expression profiles were detected by SOLiD sequencing. Differential expressions between...
Emerging evidence indicates that microRNAs (miRNAs), a class of small non‑coding RNAs, are involved in number biological processes. The results SOLiD™ sequencing were used to analyze differentially expressed miRNA profiles the plasma and placenta patients with preeclampsia (PE) subject who had pregnancy without complications. miRNAs identified consistently placenta, following normalization raw data. increased differential expression selected, as defined by percentage >0.02% log2 fold change...
Background To gain insight into potential roles of isomiR spectrum and isomiRs with 3′ additions in pre-eclampsia, we performed a comprehensive survey miRNA repertoire addition events from placental samples different degrees pre-eclampsia by applying SOLiD sequencing platform. Principal Findings Over 30% were detected non-template additional nucleotides, especially for nucleotide adenosine. However, these modified showed lower percentage total expression (<15%). Generally, 1-3 abundant given...
A core–shell SERS nanotag based VFA with a single test spot for multiplex biomarker detection at pg mL<sup>−1</sup> level wide LDR.
BackgroundCircular RNAs (circRNAs) are a class of novel with important biological functions, and aberrant expression circRNAs has been implicated in human diseases. However, the feasibility using blood as disease biomarkers is largely unknown.MethodsWe explored potential peripheral mononuclear cell (PBMC) marker molecules to diagnose active pulmonary tuberculosis (TB).FindingsFirst, we demonstrated that widely expressed PBMCs many abundant enough be detected. Second, found magnitude PBMC TB...
MicroRNAs are a class of non-coding RNAs that can be involved in the regulation gene expression cancers, including lung cancer. Our previous research has shown miR-486-5p is one most down-regulated microRNAs tissue and serum samples cancer as good diagnostic biomarker. The objective this study to investigate roles progression In study, was further validated significantly additional NSCLC tissue, cell by quantitative RT-PCR, level associated with clinical phenotype NSCLC. PIK3R1 confirmed...
Respiratory tract infections (RTIs) are severe acute infectious diseases, which require the timely and accurate identification of pathogens involved so that individual treatment plan can be selected, including optimized use antibiotics.However, high throughput ultrasensitive quantification multiple nucleic acids is a challenge in point care testing (POCT) device.Methods: Herein, we developed 2×3 microarray on lateral flow strip with surface enhanced Raman scattering (SERS) nanotags encoding...
Parkinson’s disease (PD) is the second most common neurodegenerative and although many studies have been done on this disease, underlying mechanisms are still poorly understood further warranted. Therefore, study identified circRNA expression profiles in cerebral cortex (CC), hippocampus (HP), striatum (ST), cerebellum (CB) regions of 1-methyl-1,2,3,6-tetrahydropyridine (MPTP)-induced PD mouse model using RNA sequencing (RNA-seq), differentially expressed were validated reverse transcription...
There is a great demand for the development of detection assays inflammation infection diagnosis with high throughput and ultrasensitivity. Herein, vertical flow assay system functionalized nanoporous anodic aluminum oxide (AAO) as sensing membrane, encoded core-shell surface enhanced Raman scattering (SERS) nanotags labels multiple inflammatory biomarkers presented. A 2 × test array on porous AAO developed modified capture antibodies to from samples. Due area volume ratio its influence...
Freshwater lakes are threatened by harmful cyanobacterial blooms, whose basic unit is Cyanobacterial Aggregate (CA). CA-attached bacteria play a significant role through different blooming stages with substantial variation of their taxonomic structure. However, little known about functional variations and links cyanobacteria due to the lack reference genomes. In this longitudinal study, we collected 16 CA samples from Lake Taihu, one China's largest freshwater lakes, April 2015 February...
The reliability of RNA sequencing (RNA-seq) output is affected by the quality RNAs, which in turn dependent on samples. Therefore, purposes this study were to reconsider threshold integrity number (RIN) and propose a simple efficient storage scheme blood samples for RNA-seq.The RNAs extracted from that stored at different conditions used sequencing. bioinformatic analyses performed evaluate impact sample gene expression analysis.Our outcomes showed with RIN values more than 5.3 scarcely...
Alzheimer's disease (AD) is one of the top public health crises in 21st century, especially an aging society. Early diagnosis, prevention, and intervention can significantly reduce risk AD. Detection multiple AD biomarkers blood effective strategy has drawn more attention recent years. However, concentration very low, therefore, point-of-care testing (POCT) techniques are needed for sensitive detection. Herein, a lateral flow assay, based on Surface-enhanced Raman scattering nanotags...