A5101627562- Adipose Tissue and Metabolism
- Metabolism, Diabetes, and Cancer
- Peroxisome Proliferator-Activated Receptors
- Iron Metabolism and Disorders
- Pancreatic function and diabetes
- Medical Research and Treatments
- Microbial Fuel Cells and Bioremediation
- RNA Research and Splicing
- Microbial infections and disease research
- Natural Antidiabetic Agents Studies
- Endoplasmic Reticulum Stress and Disease
- Autophagy in Disease and Therapy
- Monoclonal and Polyclonal Antibodies Research
- Pelvic and Acetabular Injuries
- Bacterial biofilms and quorum sensing
- Orthopedic Surgery and Rehabilitation
- Ferroptosis and cancer prognosis
- Clostridium difficile and Clostridium perfringens research
- Atherosclerosis and Cardiovascular Diseases
- Ocular Diseases and Behçet’s Syndrome
- Fungal Biology and Applications
- Consumer Packaging Perceptions and Trends
- Cardiac pacing and defibrillation studies
- RNA modifications and cancer
- Natural product bioactivities and synthesis
South China Agricultural University
2014-2025
Minzu University of China
2025
Ministry of Education of the People's Republic of China
2023-2024
Sichuan Agricultural University
2017-2024
Lund University
2021-2024
Zhaoqing University
2023-2024
Sun Yat-sen University Cancer Center
2024
Sun Yat-sen University
2019-2024
Sichuan Center for Disease Control and Prevention
2023-2024
Third Hospital of Changsha
2024
Scope The number of people with diabetes is increasing rapidly in the world. In present study, hypoglycemic activity and potential mechanism ergosterol (ERG), a phytosterol derived from edible mushroom Pleurotus ostreatus are investigated vitro vivo. Methods results ERG isolated identified by NMR spectra. effects on glucose uptake, transporter 4 (GLUT4) translocation, GLUT4 expression, phosphorylation AMPK, Akt PKC L6 cells evaluated. enhances uptake displays translocation up‐regulating...
As an inexpensive industrial chemical, chlorodifluoromethane (Freon-22), despite its relatively low reactivity, can serve as a practical CF
Abstract Objective Our current study aimed to assess the relationship between TNF-related apoptosis-inducing ligand (TRAIL) and ferroptosis in non-small cell lung cancer (NSCLC) development. Methods The expression of TRAIL was detected by western blot, RT-qRCR immunohistochemistry. viability NSCLC cells analyzed CCK-8 kit. migration invasion were wound healing assay transwell assay, respectively. Labile iron pool (LIP) based on calcein-acetoxymethyl ester method. Ferrous (Fe 2+ ) levels...
Diabetic kidney disease (DKD) is one of the complications diabetes mellitus, which triggers fibrosis and eventually develops into end-stage renal disease. Nuciferine (NF) most important functional components in lotus leaves (LL), but its role mechanism for treatment DKD are unclear. A high-fat-diet (HFD)-induced model KK-AY mice was established this study. NF significantly improved blood glucose biochemical indices mice. Furthermore, reduced levels mALB, UCRE, Scr, BUN urine. Further, extent...
The present study investigated the anti-diabetic activity and potential mechanisms of polyphenol rich extract from Phellinus igniarius (PI-PRE) in vitro vivo. Four main phenolic compounds PI-PRE were purified identified as 7,8-dihydroxycoumarin, 3,4-dihydroxybenzalacetone, 7,3'-dihydroxy-5'-methoxyisoflavone inoscavin C by off-line semipreparative liquid chromatography-nuclear magnetic resonance protocol. In vitro, stimulated GLUT4 translocation 2.34-fold increased glucose uptake 1.73-fold...
In bacteria, manganese homeostasis is controlled by import, regulation, and efflux. Here, we identified 2 Mn exporters, MetA MetB (manganese efflux transporters A B), in Riemerella anatipestifer CH-1, encoding a putative cation diffusion facilitator (CDF) protein resistance-nodulation-division (RND) pump, respectively. Compared with the wild type (WT), ΔmetA, ΔmetB, ΔmetAΔmetB exhibited sensitivity to manganese, since they accumulated more intracellular Mn2+ than WT under excess conditions,...
Fibrotic cataract, including posterior capsule opacification (PCO) and anterior subcapsular cataract (ASC), renders millions of people visually impaired worldwide. However, the underlying mechanism remains poorly understood. Here, we report a miRNA-based regulatory pathway that controls pathological fibrosis lens epithelium.Expression miR-22-3p histone deacetylase 6 (HDAC6) in normal PCO patient samples were measured by qPCR. Human epithelial explants treated with TGF-β2 presence or absence...
The bacterium Riemerella anatipestifer requires iron for growth, but the mechanism of uptake is not fully understood. In this study, we disrupted Feo system and characterized its function in import R. ATCC 11845. Compared to parent strain, growth ΔfeoA, ΔfeoB, ΔfeoAB strains was affected under Fe3+-limited conditions, since absence feo led less intracellular than strain. parallel, strain shown be sensitive streptonigrin, an antibiotic that free function. sensitivity hydrogen peroxide also...
Abstract Iron is essential for most bacteria to survive, but excessive iron leads damage by the Fenton reaction. Therefore, concentration of intracellular free must be strictly controlled in bacteria. Riemerella anatipestifer ( R. ), a Gram-negative bacterium, encodes uptake system. However, homeostasis mechanism remains largely unknown. In this study, it was shown that compared with wild type CH-1, CH-1Δ fur more sensitive streptonigrin, and effect alleviated when were cultured...
To compare the clinical and radiological outcomes of surgical correction Lenke type 1 to 4 scoliosis by using a multilevel Ponte osteotomy procedure with posterior selective segmental pedicle screw constructs or release only in patients adolescent thoracic idiopathic scoliosis. Retrospective analysis 65 patients, 32 treated (Group A) 33 soft tissue B). The groups were compared regard change spinal alignment from preoperative postoperative assessment over follow-up period. A rate main curve...
One of the important elements for most bacterial growth is iron, bioavailability which limited in hosts. Riemerella anatipestifer (R. anatipestifer, RA), an duck pathogen, requires iron to live. However, genes involved metabolism and mechanisms transport are largely unknown. Here, we investigated transcriptomic effects limitation condition on R. CH-1 using RNA-Seq RNA-Seq-based analysis. Data analysis revealed encoding functions related homeostasis, including a number putative TonB-dependent...
Iron is one of the most important elements for bacterial survival and pathogenicity. The iron uptake mechanism Riemerella anatipestifer (R. anatipestifer, RA), a major pathogen that causes septicemia polyserositis in ducks, largely unknown. Here, functions putative TonB-dependent transporter RA-CH-1, B739_1343, utilization pathogenicity were investigated. Under iron-starved conditions, mutant strain RA-CH-1ΔB739_1343 exhibited more seriously impaired growth than wild-type expression...
Galleria mellonella larvae have been used as a host model to study interactions between pathogens and hosts for several years. However, whether the is useful interrogate Riemerella anatipestifer infection biology remained unknown. This aimed exploit potential of G. reveal their limitations R. infection. were shown be effective virulence evaluations different strains.
Riemerella anatipestifer (R. anatipestifer, RA) is an infectious pathogen that causes septicemia and polyserositis in ducks. Our previous studies showed RA CH-1 ∆fur was significantly attenuated ducklings, which highlights the potential of this strain as a live vaccine. In study, it shown infection with 109 CFU fur mutant did not cause any clinical symptoms or significant histological lesions 3-day-old ducklings bacteria were readily cleared by host within 3 d. Compared nonvaccinated group,...
Reversible phosphorylation is an important regulatory mechanism. Regulation of protein in β-cells has been extensively investigated, but less known about dephosphorylation. To understand the role dephosphorylation and type 2 diabetes (T2D), we first examined mRNA expression 2C family (PP2C) phosphatases islets from T2D donors. Phosphatase overall was changed T2D, that PPM1E most markedly downregulated. correlated inversely with HbA1c. Silencing increased glucose-stimulated insulin secretion...
An EtOAc fraction from the roots of Caragana tangutica Maxim. (CTEA) displayed promising anti-hepatocellular carcinoma (HCC) activity during screening a traditional Chinese ethnic herb library against HepG2 and Hep3B cell lines. HPLC-based profiling CTEA by combination MS-guided large-scale semi-preparative HPLC NMR methods led to identification new pterocarpan glycoside, (-)-maackiain 3-O-6'-O-methyl malonyl-β-d-glucopyranoside (1), together with three known glycosides,...
Abstract Background The advent of proprotein convertase subtilisin/kexin type 9 (PCSK9)–inhibiting drugs have provided an effective, but extremely expensive treatment for the management low density lipoprotein (LDL). Our aim was to explore a cost‐effective application camelid anti‐PCSK9 single domain antibodies (sdAbs), which are high variable regions heavy chain (VHHs), as human PCSK9 (hPCSK9) inhibitor. One female llama immunized with hPCSK9. Screening affinity VHHs carried out based on...
Iron is an essential element for the replication of most bacteria, including Riemerella anatipestifer, a Gram-negative bacterial pathogen ducks and other birds. R. anatipestifer utilizes hemoglobin-derived hemin as iron source; however, mechanism by which this bacterium acquires from hemoglobin largely unknown. Here, rhuA disruption was shown to impair utilization duck in CH-1. Moreover, putative lipoprotein RhuA identified surface-exposed, outer membrane hemin-binding protein, but it could...
Riemerella anatipestifer encodes an iron acquisition system, but whether it the efflux pump and its role in antibiotic resistance are largely unknown.