A5007241601- Spine and Intervertebral Disc Pathology
- Musculoskeletal pain and rehabilitation
- Cervical and Thoracic Myelopathy
- Osteoarthritis Treatment and Mechanisms
- Spinal Cord Injury Research
- Spinal Hematomas and Complications
- Cancer-related molecular mechanisms research
- Pregnancy-related medical research
- Bone Metabolism and Diseases
- Circular RNAs in diseases
- Pain Mechanisms and Treatments
- Adenosine and Purinergic Signaling
- Higher Education and Teaching Methods
- Immune cells in cancer
- Tendon Structure and Treatment
- Bone and Joint Diseases
- MicroRNA in disease regulation
- Neuroscience of respiration and sleep
- Circadian rhythm and melatonin
- Spondyloarthritis Studies and Treatments
- Muscle Physiology and Disorders
- interferon and immune responses
- Pharmacological Effects of Natural Compounds
- Plant Toxicity and Pharmacological Properties
- Spinal Fractures and Fixation Techniques
First Affiliated Hospital of Anhui Medical University
2022-2025
Anhui Medical University
2022-2025
Chengdu Sport University
2021-2025
Washington University in St. Louis
2022-2024
Centre international de recherche sur l’environnement et le développement
2023-2024
Guangzhou University of Chinese Medicine
2024
Huazhong University of Science and Technology
2016-2023
Tianjin Medical University General Hospital
2019-2022
Union Hospital
2016-2020
National Natural Science Foundation of China
2019
Mitochondrial dysfunction, oxidative stress and nucleus pulposus (NP) cell apoptosis are important contributors to the development pathogenesis of intervertebral disc degeneration (IDD). Here, we comprehensively evaluated effects mitochondrial dynamics, mitophagic flux Nrf2 signalling on quality control, ROS production NP survival in vitro ex vivo compression models IDD explored mitochondria-targeted anti-oxidant MitoQ its mechanism.Human cells were exposed mechanical mimic pathological...
Intervertebral disc (IVD) degeneration contributes largely to pathoanatomical and degenerative changes of spinal structure that increase the risk low back pain. Apoptosis in nucleus pulposus (NP) can aggravate IVD degeneration, increasing studies have shown interventions targeting NP cell apoptosis ameliorate exhibiting their potential for use as therapeutic strategies. Recent data advanced glycation end products (AGEs) accumulate tissues parallel with progression form a microenvironment...
Intervertebral disc degeneration is widely recognized as a cause of lower back pain, neurological dysfunction and other musculoskeletal disorders. The major inflammatory cytokine IL-1β associated with intervertebral degeneration; however, the molecular mechanisms that drive production in disc, especially nucleus pulposus (NP) cells, are unknown. In some tissues, advanced glycation end products (AGEs), which accumulate NP tissues promote its degeneration, increase oxidative stress secretion,...
Oxidative stress-induced mitochondrial dysfunction and nucleus pulposus (NP) cell apoptosis play crucial roles in the development of intervertebral disc degeneration (IDD). Increasing studies have shown that interventions targeting impaired autophagic flux can maintain cellular homeostasis by relieving oxidative damage. Here, we investigated effect curcumin (CUR), a known autophagy activator, on IDD vitro vivo. CUR suppressed tert-butyl hydroperoxide- (TBHP-) induced stress thereby inhibited...
BackgroundIntervertebral disc degeneration (IDD) is a major contributor to lower back pain, however, the molecular and pathogenetic mechanisms underlying IDD are poorly understood. As high-risk factor for IDD, compression stress was reported induce apoptosis of nucleus pulposus (NP) cells extracellular matrix (ECM) degradation during progression. Circular RNA (circRNA) class endogenous non-coding (ncRNA) has been function in several diseases. However, whether how circRNA regulates...
Intervertebral disc degeneration (IVDD) is the leading cause of low back pain, and repair using nucleus pulposus-derived mesenchymal stem cells (NP-MSCs) represents a promising therapeutic approach. However, both endogenous transplanted NP-MSCs demonstrate limited proliferative capacity, increased apoptosis, reduced resilience to harsh microenvironment within degenerative intervertebral (IVD). RNA sequencing (RNA-seq) was utilized identify genes associated mechanisms that mediate responses...
Background: Low back pain is a common problem, mainly caused by intervertebral disc degeneration (IDD). An important pathophysiological characteristic of IDD the loss homeostatic balance extracellular matrix metabolism. Interleukin-1β (IL-1β) one inflammatory mediators stimulating degradation in nucleus pulposus (NP) and contributing to pathogenesis. Icariin, which isolated from Epimedium brevicornum, acts as an anti-inflammatory drug. Objective: This study aimed explore pharmacological...
MicroRNAs play an important role in the etiology and progression of many diseases, including intervertebral disc degeneration (IVDD). The miRNA miR-129-5P regulates autophagy various cancers, but its human nucleus pulposus (NP) cells is unclear. present study investigated whether miR-129-5p expression Beclin-1 which known to induce NP by evaluating their levels normal degenerative tissues transfected with mimic or inhibitor quantitative real-time (qRT-)PCR, western blotting, flow cytometry,...
This study investigated the expression and function of microRNA-494 in intervertebral disc degeneration (IDD).MicroRNA-494 was upregulated during IDD progression; its overexpression increased ECM catabolic factors such as matrix metalloproteinase A disintegrin with thrombospondin motif NP cells while decreasing that anabolic genes type II collagen aggrecan; it also induced apoptosis cells, determined by flow cytometry. These effects were reversed inhibitor treatment. SOX9 identified a target...
Previous studies have indicated the important roles of ADAMTS5 in intervertebral disc degeneration (IDD). However, mechanisms that regulate expression nuclear pulposus (NP) cells remain largely unknown. Evidence suggests intergenic transcription may be associated with genes encode transcriptional regulators. Here, we identified a long noncoding RNA, linc-ADAMTS5, which was transcribed opposite direction to ADAMTS5. In present study, through mining computational algorithm programs, and...