A5100373648- Monoclonal and Polyclonal Antibodies Research
- T-cell and B-cell Immunology
- Anesthesia and Pain Management
- Lipoproteins and Cardiovascular Health
- Advanced MRI Techniques and Applications
- Biosimilars and Bioanalytical Methods
- SARS-CoV-2 and COVID-19 Research
- Coastal wetland ecosystem dynamics
- Plant responses to water stress
- Diabetes and associated disorders
- Land Use and Ecosystem Services
- Synthesis of β-Lactam Compounds
- Glycosylation and Glycoproteins Research
- Polymer Synthesis and Characterization
- Stress Responses and Cortisol
- Animal Virus Infections Studies
- Molecular Biology Techniques and Applications
- Extracellular vesicles in disease
- Immunotherapy and Immune Responses
- Pancreatic and Hepatic Oncology Research
- Neuroendocrine regulation and behavior
- Mechanical Circulatory Support Devices
- Medical Imaging Techniques and Applications
- Veterinary medicine and infectious diseases
- Advanced Neuroimaging Techniques and Applications
Linyi People's Hospital
2025
First Affiliated Hospital of Xi'an Jiaotong University
2024
Tongji University
2024
Henan University of Urban Construction
2022-2024
Nanjing Normal University
2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2022
BGI Group (China)
2016-2021
University of Chinese Academy of Sciences
2018-2021
China National GeneBank
2019-2021
Sichuan Agricultural University
2020
Compared to classical antibodies, camel heavy chain antibodies (HCAbs) are smaller in size due lack of the light and first constant domain (CH1 region). The variable regions HCAbs (VHHs) more soluble stable than that conventional (VHs). Even with such simple structure, they still functional antigen binding. Although have been extensively investigated over past two decades, most efforts based upon low throughput sequence analysis, there only limited reports trying analyze describe complete...
Rebound pain after regional anesthesia, a common phenomenon when the analgesic effect wears off, has been recognized in last few years. The aim of this study is to analyze status and tendency area macroscopic perspective.
Objective: To investigate the median effective dose (ED50) of ropivacaine for unilateral spinal anesthesia in total knee arthroplasty (TKA) elderly patients with different heights. Methods: Sixty ASA ⅱ-ⅲ patients, aged ≥ 60 yr, BMI 20.0–29.9 kg/m2, undergoing under anesthesia, were enrolled this study. The divided into three groups according to their height. height was 151–155 cm, which recorded as S group. Group M (height 156–160 cm); H 161–165 cm), puncture performed L3-4 space, and 0.25%...
Abstract Background The advent of proprotein convertase subtilisin/kexin type 9 (PCSK9)–inhibiting drugs have provided an effective, but extremely expensive treatment for the management low density lipoprotein (LDL). Our aim was to explore a cost‐effective application camelid anti‐PCSK9 single domain antibodies (sdAbs), which are high variable regions heavy chain (VHHs), as human PCSK9 (hPCSK9) inhibitor. One female llama immunized with hPCSK9. Screening affinity VHHs carried out based on...
Postoperative delirium (POD), a common complication affecting short- and long-term prognosis in elderly patients, leads to heavy burden on social economy health care. The main purpose of this study is conduct bibliometric analysis the 100 most frequently cited articles POD.
Background Pseudo-continuous arterial spin labeling (pCASL) is widely used to quantify cerebral blood flow (CBF) abnormalities in patients with Alzheimer’s disease (AD). T1-mapping techniques assess microstructural characteristics various pathologic changes, but their application AD remains the exploratory stage. We hypothesized that combining quantitative CBF and T1 values would generate diagnostic results higher accuracy than using either method alone discriminating from cognitively normal...
Proprotein convertase subtilisin/kexin type 9 (PCSK9) has emerged as a promising therapeutic target to reduce lipids. In 2020, we reported chimeric camelid-human heavy chain antibody VHH-B11-Fc targeting PCSK9. Recently, it was verified that VHH-B11 binds one linear epitope in the PCSK9 hinge region. To enhance its druggability, have developed novel biparatopic B11-H2-Fc Ab herein. Thereinto, surface plasmon resonance (SPR) confirmed differences binding-PCSK9 among VHH-B11, VHH-H2 and...
In this report, one novel method has been developed to screen the monoclonal antibody against human pancreatic cancer biomarker glypican-1 (GPC1) through combination of fluorescent cell sorting and single B amplification. GPC1-positive cells were sorted out from peripheral blood mononuclear (PBMCs) by after GPC1 immunization New Zealand white rabbit. Then, total RNA was extracted reversely transcribed into cDNA, which used as template, variable region sequences both heavy light chains...
Abstract PCSK9 is an effective target for lowering LDL-c. Previously, a camelid-human chimeric heavy chain antibody VHH-B11-Fc targeting human was designed. It had potent hypolipidemic effect. However, the nanobody VHH-B11 interacts with at low affinity, while camelid VHH exhibits some immunogenicity. Moreover, interacting epitope yet to be identified, although shown have distinct hPCSK9-binding epitopes Evolocumab. This might impede molecule’s progress from bench bedside. In present study,...
ABSTRACT Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused the pandemic of disease 2019 (COVID-19). Great international efforts have been put into development prophylactic vaccines and neutralizing antibodies. However, knowledge about B cell immune response induced by SARS-CoV-2 virus is still limited. Here, we report a comprehensive characterization dynamics immunoglobin heavy chain (IGH) repertoire in COVID-19 patients. By using next-generation sequencing technology,...
Ginger, an important cash crop, has been cultivated for thousands of years in China. However, comparative studies on soil bacterial communities Chinese ginger varieties, especially after flooding, are lacking. Here, we comprehensively compared the two types soils from four different locations. Surprisingly, 100-year flood (20 July 2021, Henan, China) did not significantly affect composition with previous reports. In contrast, flooding may have brought nutrients and promoted propagation...
Abstract Background: Transfer factors (TFs), a novel immunostimulatory reagent, have found use as auxiliary or primary therapy for many diseases. The aim of this study was to explore whether TFs are able strengthen immune responses Newcastle Disease (ND) vaccines in broilers. Results: serum antioxidant status increased TF-treatment TF subcutaneous injection could significantly increase (P < 0.5) the antibody titers at 14 and 21 days experiment. Moreover, treatment development organs...
Increased evidence shows that the pathogenesis of Kawasaki disease (KD) is caused by abnormal and unbalanced innate adaptive immune responses. However, changes in functions cells KD peripheral blood are still controversial. In this study, three different methods, CIBERSORT, Immune Cell Abundance Identifier (ImmuCellAI), cell markers, were used to evaluate proportions abundances eight datasets (GSE9863, GSE9864, GSE18606, GSE63881, GSE68004, GSE73461, GSE73463, GSE64486; a total 1,251...