A5026174761- Cytokine Signaling Pathways and Interactions
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Immune Response and Inflammation
- Chemical Synthesis and Reactions
- Radical Photochemical Reactions
- Advanced biosensing and bioanalysis techniques
- Adenosine and Purinergic Signaling
- Vitamin K Research Studies
- Influenza Virus Research Studies
- Sulfur-Based Synthesis Techniques
- Luminescence and Fluorescent Materials
- Immune Cell Function and Interaction
- Peptidase Inhibition and Analysis
- Osteoarthritis Treatment and Mechanisms
- Eosinophilic Esophagitis
- Immune cells in cancer
- Biochemical and Structural Characterization
- Bone Metabolism and Diseases
- Inflammatory mediators and NSAID effects
- Signaling Pathways in Disease
- Growth Hormone and Insulin-like Growth Factors
- IL-33, ST2, and ILC Pathways
- Digestive system and related health
- Advanced Nanomaterials in Catalysis
- Nanoplatforms for cancer theranostics
University of Edinburgh
2022-2025
MRC Centre for Regenerative Medicine
2023-2025
Centre for Inflammation Research
2022-2025
University of Sheffield
2019
Centre for Life
2019
Newcastle University
2019
Immunosuppressants are clinically approved drugs to treat the potential rejection of transplanted organs and require frequent monitoring due their narrow therapeutic window. Immunophilins small proteins that bind immunosuppressants with high affinity, yet there no examples fluorogenic immunophilins application as optical biosensors for immunosuppressive in clinical biosamples. In present work, we designed novel diazonium BODIPY salts site-specific labeling tyrosine residues peptides via...
The essential functions that cytokine/immune cell interactions play in tissue homeostasis and during disease have prompted the molecular design of targeted fluorophores to monitor their activity real time. Whereas activatable probes for imaging immune-related enzymes are common, many immunological mediated by binding events between cytokines cognate receptors hard live-cell imaging. A prime example is interleukin-33 (IL-33), a key cytokine innate adaptive immunity, whose interaction with ST2...
Photocatalysts are excellent scaffolds for the light-mediated control of bioactive molecules. Current photocatalytic structures not compatible with genetic encoding and therefore cannot be directly incorporated into sequences native proteins. Herein, we developed new amino acids incorporating Si-rosamine units, introduced them via aminoacylation tRNAs specific positions different proteins to enable targeted reactions in defined populations immune cells.
Osteoarthritis (OA) is a common disease of older individuals that impacts detrimentally on the quality and length life. It characterised by painful loss articular cartilage polygenic multifactorial. Genome-wide association scans have highlighted over 90 osteoarthritis genetic signals, some which reside within or close to highly plausible candidate genes. An example an polymorphisms adjacent matrix Gla protein gene MGP. We set out undertake functional study this gene. Nucleic acid was...
Neutrophilic inflammation with prolonged neutrophil survival is common to many inflammatory conditions, including chronic obstructive pulmonary disease (COPD). There are few specific therapies that reverse neutrophilic inflammation, but uncovering mechanisms regulating likely identify novel therapeutic targets. Screening of 367 kinase inhibitors in human neutrophils and a zebrafish tail fin injury model identified ErbBs as targets compounds accelerated resolution. The ErbB gefitinib,...
Non-typeable Haemophilus influenzae (NTHi) is a frequent cause of lower respiratory tract infection in people with chronic obstructive pulmonary disease (COPD). Pellino proteins are family E3 ubiquitin ligases that critical regulators TLR signaling and inflammation. The aim this study was to identify role for Pellino-1 airway defense against NTHi the context COPD. rapidly upregulated by LPS monocyte-derived macrophages (MDMs) isolated from individuals COPD healthy control subjects, TLR4...
Five new disubstituted 2,6-thioaryl-BODIPY dyes were synthesized via selective aromatic electrophilic substitution from commercially available thiophenols. The analysis of the photophysical properties absorption and emission spectroscopy showed unusually large Stokes shifts for BODIPY fluorophores (70–100 nm), which makes them suitable probes bioimaging. Selected compounds evaluated labelling primary immune cells as well different cancer cell lines using confocal fluorescence microscopy.
Abstract Neutrophilic inflammation with prolonged neutrophil survival is common to many inflammatory conditions, including chronic obstructive pulmonary disease (COPD). There are few specific therapies that reverse neutrophilic inflammation, but uncovering mechanisms regulating likely identify novel therapeutic targets. Screening of 367 kinase inhibitors in human neutrophils and a zebrafish tail fin injury model identified ErbBs as targets compounds accelerated resolution. The ErbB...