A5008556973- Bacterial biofilms and quorum sensing
- Bacterial Genetics and Biotechnology
- Vibrio bacteria research studies
- Biochemical and Structural Characterization
- Immune Cell Function and Interaction
- Cardiovascular and exercise physiology
- Antimicrobial Resistance in Staphylococcus
- Heart Rate Variability and Autonomic Control
- Cardiovascular Effects of Exercise
- Reproductive System and Pregnancy
- RNA and protein synthesis mechanisms
- Genomics and Phylogenetic Studies
- Cardiac Health and Mental Health
- Microbial Metabolic Engineering and Bioproduction
- Microbial Metabolism and Applications
- Advanced Biosensing Techniques and Applications
- Lipid Membrane Structure and Behavior
- Advanced biosensing and bioanalysis techniques
- Photosynthetic Processes and Mechanisms
- Microbial Community Ecology and Physiology
- T-cell and B-cell Immunology
- Immune cells in cancer
- IL-33, ST2, and ILC Pathways
Harvard University
2018-2024
New York State Department of Health
2024
Boston VA Research Institute
2023-2024
Princeton University
2017-2021
Logan Hospital
2018-2020
Rider University
2010-2012
Abstract Staphylococcus aureus is a Gram-positive pathogen responsible for antibiotic-resistant infections. To identify vulnerabilities in cell envelope biogenesis that may overcome resistance, we enriched S. transposon mutants with defects surface integrity or division by sorting cells stain propidium iodide have increased light-scattering properties, respectively. Transposon sequencing of the sorted populations identified more than 20 previously uncharacterized factors impacting these...
Collective behavior in spatially structured groups, or biofilms, is the norm among microbes their natural environments. Though biofilm formation has been studied for decades, tracing mechanistic and ecological links between individual cell morphologies emergent features of groups still its infancy. Here we use single-cell–resolution confocal microscopy to explore biofilms human pathogen Vibrio cholerae conditions mimicking marine habitat. Prior reports have noted occurrence cellular...
SUMMARY Staphylococcus aureus is a gram-positive pathogen responsible for life-threatening infections that are difficult to treat due antibiotic resistance. The identification of new vulnerabilities in essential processes like cell envelope biogenesis represents promising avenue towards the development anti-staphylococcal therapies overcome To this end, we performed sorting-based enrichments S. mutants with defects integrity and division. We identified many known factors as well large...
Abstract Bacteria can achieve a staggering diversity of cell shapes that promote critical functions like growth, motility, and virulence 1-4 . Previous studies suggested bacteria establish complex by co-opting the core machineries essential for elongation division 5,6 In contrast, we discovered two-protein module, CrvAB, curve autonomously major machinery forming dynamic, asymmetrically-localized structure in periplasm. CrvAB is curvature its native species, Vibrio cholerae , sufficient to...
Abstract Collective behavior in spatially structured groups, or biofilms, is the norm among microbes their natural environments. Though microbial physiology and biofilm formation have been studied for decades, tracing mechanistic ecological links between individual cell properties emergent features of groups still its infancy. Here we use single-cell resolution confocal microscopy to explore human pathogen Vibrio cholerae conditions closely mimicking marine habitat. We find that some – but...