A5069200938- SARS-CoV-2 and COVID-19 Research
- Bacterial Genetics and Biotechnology
- Vibrio bacteria research studies
- SARS-CoV-2 detection and testing
- Biosensors and Analytical Detection
- RNA Research and Splicing
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Bacterial biofilms and quorum sensing
- Bacteriophages and microbial interactions
- Cancer Research and Treatments
- Advanced biosensing and bioanalysis techniques
- Respiratory viral infections research
- COVID-19 Clinical Research Studies
- Infection Control and Ventilation
- Legionella and Acanthamoeba research
- Vitamin C and Antioxidants Research
- RNA Interference and Gene Delivery
- Viral gastroenteritis research and epidemiology
- Enterobacteriaceae and Cronobacter Research
- Computational Drug Discovery Methods
- Lipid Membrane Structure and Behavior
Friedrich Schiller University Jena
2020-2022
Institute of Bioinformatics and Systems Biology
2021-2022
Bayerisches Landesamt für Gesundheit und Lebensmittelsicherheit
2022
Ludwig-Maximilians-Universität München
2019-2020
Czech Academy of Sciences, Institute of Microbiology
2020
Abstract Background During the ongoing Covid-19 pandemic caused by emerging virus SARS-CoV-2, research in field of coronaviruses has expanded tremendously. The genome SARS-CoV-2 rapidly acquired numerous mutations, giving rise to several Variants Concern (VOCs) with altered epidemiological, immunological, and pathogenic properties. Methods As cell culture models are important tools study viruses, we investigated replication kinetics infectivity African Green Monkey-derived Vero E6 kidney...
Collective behavior in spatially structured groups, or biofilms, is the norm among microbes their natural environments. Though biofilm formation has been studied for decades, tracing mechanistic and ecological links between individual cell morphologies emergent features of groups still its infancy. Here we use single-cell–resolution confocal microscopy to explore biofilms human pathogen Vibrio cholerae conditions mimicking marine habitat. Prior reports have noted occurrence cellular...
Negative feedback regulation, that is the ability of a gene to repress its own synthesis, most abundant regulatory motif known biology. Frequently reported for transcriptional regulators, negative control relies on binding transcription factor promoter. Here, we report novel mechanism autoregulation in bacteria relying small RNA (sRNA) and major endoribonuclease, RNase E. TIER-seq analysis (transiently-inactivating-an-endoribonuclease-followed-by-RNA-seq) revealed ~25,000 E-dependent...
SARS-CoV-2 variants of concern (VOC) should not escape molecular surveillance. We investigated if rapid antigen tests (RATs) could detect B.1.1.7 and B.1.351 VOCs in certain laboratory conditions. Infectious cell culture supernatants containing B.1.1.7, or non-VOC were respectively diluted both DMEM saliva. Dilutions analysed with Roche, Siemens, Abbott, nal von minden RapiGEN RATs. While further studies appropriate real-life clinical samples are warranted, all RATs detected B.1.351,...
Small regulatory RNAs (sRNAs) acting in concert with the RNA chaperone Hfq are prevalent many bacteria and typically act by base-pairing multiple target transcripts. In human pathogen Vibrio cholerae, sRNAs play roles various processes including antibiotic tolerance, competence, quorum sensing (QS). Here, we use RIL-seq (RNA-interaction-by-ligation-and-sequencing) to identify Hfq-interacting their targets V. cholerae. We find hundreds of sRNA-mRNA interactions, as well duplexes formed...
Article17 July 2019Open Access Source DataTransparent process A conserved RNA seed-pairing domain directs small RNA-mediated stress resistance in enterobacteria Nikolai Peschek orcid.org/0000-0003-2835-775X Faculty of Biology I, Department Microbiology, Ludwig-Maximilians-University Munich, Martinsried, Germany Munich Center for Integrated Protein Science (CIPSM), Search more papers by this author Mona Hoyos Roman Herzog Konrad U Förstner Institute Information Science, TH Köln – University...
Rapid antigen tests (RATs) are an integral part of SARS-CoV-2 containment strategies. As emerging variants concern (VOCs) displace the initially circulating strains, it is crucial that RATs do not fail to detect these new variants. In this study, four for nasal swab testing were investigated using cultured strains B.1.1 (non-VOC), B.1.1.7 (Alpha), B.1.351 (Beta), P.1 (Gamma), and B.1.617.2 (Delta). Based on dilution series in cell culture medium pooled saliva, limit detection was determined...
Bacterial small RNAs (sRNAs) are well known to modulate gene expression by base pairing with trans-encoded transcripts and typically non-coding. However, several sRNAs have been reported also contain an open reading frame thus considered dual-function RNAs. In this study, we discovered a RNA from Vibrio cholerae, called VcdRP, harboring 29 amino acid protein (VcdP), as base-pairing sequence. Using forward genetic screen, identified VcdRP repressor of cholera toxin production link phenotype...
Omicron is rapidly spreading as a new SARS-CoV-2 variant of concern (VOC). The question whether this has an impact on rapid antigen test (RAT) performance utmost importance. To obtain initial estimate regarding differences RATs in detecting omicron and delta, seven commonly used from different manufacturers were analysed using cell culture supernatants clinical specimens.
As the MHC-I-pathway is key to antigen presentation cytotoxic T-cells and, therefore, recognition by host adaptive immune system, we hypothesized that SARS-CoV-2 including its Variants of Concern (VOCs), influences MHC-I expression on epithelial cell surfaces as an evasion strategy. We conducted in vitro time course experiment with human airway line Calu-3 and colorectal adenocarcinoma Caco-2. Cells were infected strains non-VOC/B.1.1, Alpha/B.1.1.7, Beta/B.1.351, Gamma/P.1, Delta/B.1.617.2....
The emergence of SARS-CoV-2 in 2019 led to a severe pandemic situation. Treatment options are limited, and the efficacy vaccines decreases due mutations strains. Therefore, new treatment urgently needed, computational compound screenings used predict drugs quickly. One these revealed farnesyltransferase inhibitors (FTIs) as potential candidates. infected Calu-3 cells were treated with lonafarnib tipifarnib fold change viral replication was measured using RT-qPCR. Furthermore, morphological...