A5101413377- Glycosylation and Glycoproteins Research
- Vibrio bacteria research studies
- Advanced Proteomics Techniques and Applications
- Long-Term Effects of COVID-19
- Retinal and Optic Conditions
- Cancer, Hypoxia, and Metabolism
- COVID-19 Clinical Research Studies
- Enzyme Structure and Function
- Bacterial Genetics and Biotechnology
- Aquaculture disease management and microbiota
- Zebrafish Biomedical Research Applications
- Topic Modeling
- Biosimilars and Bioanalytical Methods
- 3D Printing in Biomedical Research
- Monoclonal and Polyclonal Antibodies Research
- Ubiquitin and proteasome pathways
- Extracellular vesicles in disease
- Pasture and Agricultural Systems
- Escherichia coli research studies
- Gene Regulatory Network Analysis
- thermodynamics and calorimetric analyses
- Advanced Nanomaterials in Catalysis
- Infant Nutrition and Health
- Pesticide Residue Analysis and Safety
- Biotin and Related Studies
University of California, Davis
2019-2025
Hunan University of Science and Technology
2025
Air Force Medical University
2024
University of California System
2021
Indiana University – Purdue University Indianapolis
2020
Nanjing Agricultural University
2006-2017
Seattle University
2017
University of Washington
2017
National Institute for Communicable Disease Control and Prevention
2015-2016
Peking University
2012-2013
Protein–protein interactions in the cell membrane are typically mediated by glycans, with terminal sialic acid often involved these interactions. To probe nature of interactions, we developed quantitative cross-linking methods involving glycans glycoproteins and polypeptide moieties proteins. We designed synthesized biotinylated enrichable cross-linkers that were click-tagged to metabolically incorporate azido-sialic on surface allow azido-glycans lysine residues proximal polypeptides. The...
ABSTRACT Zinc is an essential trace metal required for numerous cellular processes in all forms of life. In order to maintain zinc homeostasis, bacteria have developed several transport systems regulate its uptake. this study, we investigated the enteric pathogen Vibrio cholerae , causative agent cholera. Bioinformatic analysis predicts that two gene clusters, VC2081 VC2083 (annotated as utilization genes znuABC ) and VC2551 VC2555 z inc- r egulated g enes zrgABCDE ), are regulated by...
Definitive hematopoietic stem and progenitor cells (HSPCs) arise from the transdifferentiation of hemogenic endothelial (hemECs). The mechanisms this endothelial-to-hematopoietic transition (EHT) are poorly understood. We show that microRNA-223 (miR-223)-mediated regulation N-glycan biosynthesis in (ECs) regulates EHT. miR-223 is enriched hemECs oligopotent nascent HSPCs. restricts EHT lymphoid-myeloid lineages by suppressing mannosyltransferase alg2 sialyltransferase st3gal2, two enzymes...
A lectin proximity oxidative labeling (Lectin PROXL) tool was developed to identify cell surface glycoproteins that contain glycans are recognized by lectins.
The cell surface glycocalyx is highly interactive defined by extensive covalent and non-covalent interactions. A method for cross-linking characterizing glycan–peptide interactions <italic>in situ</italic> developed.
Glycans on the host cell membrane and viral proteins play critical roles in pathogenesis. Highly glycosylated epithelial cells represent primary boundary separating embedded tissues from pathogens within respiratory intestinal tracts. SARS-CoV-2, causative agent for COVID-19 pandemic, reaches into tract. We found purified human milk oligosaccharides (HMOs) inhibited binding cells. Spike (S) protein receptor domain (RBD) to were partly blocked by co-incubation with exogenous HMOs, most...
Cell membrane glycoproteins are generally highly fucosylated and sialylated, post-translational modifications play important roles in the proteins' functions of signaling, binding cellular processing. For these reasons, methods for measuring sialic acid-mediated protein-protein interactions have been developed. However, determining role fucose has limited by technological barriers that thus far hindered ability to characterize observe fucose-mediated interactions. Herein, we describe a...
Bacterial pathogens display versatile gene expression to adapt changing surroundings. For example, Vibrio cholerae, the causative agent of cholera, utilizes distinct genetic programs combat reactive oxygen species (ROS) in aquatic environments or during host infection. We previously reported that virulence activator AphB V. cholerae is involved ROS resistance. Here by performing a screen, we show represses resistance ohrA, which also repressed another regulator, OhrR. Reduced forms both and...
The viscoelastic mucus layer of gastrointestinal tracts is a host defense barrier that successful enteric pathogen, such as Vibrio cholerae, must circumvent. V. the causative agent cholera, able to penetrate mucosa and colonize epithelial surface small intestine. In this study, we found mucin, major component mucus, promoted cholerae movement on semisolid medium in liquid medium. A genome-wide screen revealed polysaccharide (VPS) production was inversely correlated with mucin-enhanced...
A novel, simple and sensitive method based on the use of a PRiME (process, robustness, improvements, matrix effects, ease use) pass-through cleanup procedure combined with ultra-fast liquid chromatography-tandem quadrupole mass spectrometry (UFLC-MS/MS) for determination 4 AFs, i.e., AFB1, AFB2, AFG1 AFG2 in human serum, was developed validated. The effective using Oasis HLB cartridge optimized extraction serum. obtained results demonstrated higher capacity recoveries between 92.1% 102%....
To explore the biological process of liver tissue-derived extracellular vesicle (LT-EV) in promoting osteogenic differentiation mesenchymal stem cells and healing jaw defects to provide a feasible treatment method for clinical bone defects.
Background The rapid coronavirus disease 2019 (COVID-19) pandemic has hit hard on the world and causes panic since virus serious respiratory infectious illness easily leads to severe conditions such as immune system overactivation or cytokine storm. Due limited knowledge of this course a lack an effective treatment for fatal disease, mortality remains high. emergence storm in patients condition been reported top reason death with COVID-19 infection. However, causative mechanism elusive....
Abstract Glycosylation is a conserved post-translational modification by which oligosaccharides are attached to macromolecule, typically proteins and lipids found on the cell surface. In particular, protein glycosylation play an important role in host-microbe interactions can further help describing microbial pathogenicity, recognition host immune system susceptibility infections. Glycan-mediated were empirically investigated using glycan arrays; however, characterizing cellular glycocalyx...
A central goal of synthetic biology is to apply successful principles that have been developed in electronic and chemical engineering construct basic biological functional modules, through rational design, build systems with predetermined functions. Here, we the reverse design principle networks synthesize a gene circuit executes semi‐log dose‐response, logarithmically linear sensing function, Escherichia coli cells. We first mathematically define object function then search for tri‐node...
The stable isotope deuterium dose-to-mother (DTM) technique to estimate nonbreast milk water intake demonstrates that maternal self-report methods of infant feeding overestimate the true prevalence exclusively breastfeeding practices. We aimed determine potential monosaccharide and oligosaccharide markers distinguish between breastfed (EBF) versus nonexclusively (non-EBF) infants utilizing LC-MS-based methods. Data for analysis were collected as part a larger, longitudinal study 192...
Evaluating aligned large language models' (LLMs) ability to recognize and reject unsafe user requests is crucial for safe, policy-compliant deployments. Existing evaluation efforts, however, face three limitations that we address with SORRY-Bench, our proposed benchmark. First, existing methods often use coarse-grained taxonomies of topics, are over-representing some fine-grained topics. For example, among the ten datasets evaluated, tests refusals self-harm instructions over 3x less...
SUMMARY Protein-protein interactions (PPIs) provide essential insights into the complex molecular mechanisms and signaling pathways within cells that regulate development disease-related phenotypes. However, for membrane proteins, impact of various forms glycosylation has often been overlooked in PPI studies. In this study, we introduce a novel approach, glycan-dependent affinity purification followed by mass spectrometry (GAP-MS), to assess variations PPIs any glycoprotein interest under...
Abstract Hematopoietic stem and progenitor cells (HSPCs) that establish maintain the blood system in adult vertebrates arise from transdifferentiation of hemogenic endothelial (hemECs) during embryogenesis. This endothelial-to-hematopoietic transition (EHT) is tightly regulated, but mechanisms are poorly understood. Here, we show microRNA (miR)-223-mediated regulation N-glycan biosynthesis (ECs) regulates EHT. Single cell RNA-sequencing revealed miR-223 enriched hemECs oligopotent nascent...