A5005677847- Extracellular vesicles in disease
- RNA Interference and Gene Delivery
- MicroRNA in disease regulation
- Cell Adhesion Molecules Research
- Mesenchymal stem cell research
- Circular RNAs in diseases
- Chronic Lymphocytic Leukemia Research
- Advanced biosensing and bioanalysis techniques
- Immunodeficiency and Autoimmune Disorders
- Neonatal Respiratory Health Research
- Adipose Tissue and Metabolism
- Nanoplatforms for cancer theranostics
- Exercise and Physiological Responses
- RNA regulation and disease
- Virus-based gene therapy research
- RNA modifications and cancer
- Complement system in diseases
- Immune cells in cancer
- Neurogenetic and Muscular Disorders Research
- Nanopore and Nanochannel Transport Studies
- Lipid Membrane Structure and Behavior
- Characterization and Applications of Magnetic Nanoparticles
- CRISPR and Genetic Engineering
- Respiratory Support and Mechanisms
- Galectins and Cancer Biology
Karolinska Institutet
2015-2025
Karolinska University Hospital
2014-2025
PsiOxus Therapeutics (United Kingdom)
2018-2023
Extracellular vesicles (EVs) have emerged as important mediators of intercellular communication in a diverse range biological processes. For future therapeutic applications and for EV biology research general, understanding the vivo fate EVs is utmost importance. Here we studied biodistribution mice after systemic delivery. were isolated from 3 different mouse cell sources, including dendritic cells (DCs) derived bone marrow, labelled with near-infrared lipophilic dye. Xenotransplantation...
Extracellular vesicles (EVs) are natural nanoparticles that mediate intercellular transfer of RNA and proteins great medical interest; serving as novel biomarkers potential therapeutic agents. However, there is little consensus on the most appropriate method to isolate high-yield high-purity EVs from various biological fluids. Here, we describe a systematic comparison between two protocols for EV purification: ultrafiltration with subsequent liquid chromatography (UF-LC) differential...
Alpha-synuclein (α-Syn) aggregates are the main component of Lewy bodies, which characteristic pathological feature in Parkinson's disease (PD) brain. Evidence that α-Syn aggregation can be propagated between neurones has led to suggestion this mechanism is responsible for stepwise progression PD pathology. Decreasing expression predicted attenuate process and thus an attractive approach delay or halt progression. We have used small interfering RNA (siRNA) reduce total aggregated levels...
Abstract Extracellular vesicles (EVs) play a key role in many physiological and pathophysiological processes hold great potential for therapeutic diagnostic use. Despite significant advances within the last decade, issue of EV storage stability remains unresolved under investigated. Here, we aimed to identify conditions stabilizing EVs comprehensively compared impact various buffer formulations at different temperatures on derived from cellular sources up 2 years. features including...
Extracellular vesicles (EVs) can be harvested from cell culture supernatants and all body fluids. EVs conceptually classified based on their size biogenesis as exosomes microvesicles. Nowadays, it is however commonly accepted in the field that there a much higher degree of heterogeneity within these two subgroups than previously thought. For instance, surface marker profile likely dependent source, cell's activation status, multiple other parameters. Within recent years, several new methods...
Extracellular vesicles (EVs) are naturally occurring nano-sized carriers that secreted by cells and facilitate cell-to-cell communication their unique ability to transfer biologically active cargo. Despite the pronounced increase in our understanding of EVs over last decade, from disease pathophysiology therapeutic drug delivery, improved molecular tools track delivery still needed. Unfortunately, present catalogue utilised for EV labelling lacks sensitivity or not sufficiently specific....
Extracellular vesicles (EVs) function as natural delivery vectors and mediators of biological signals across tissues. Here, by leveraging these functionalities, we show that EVs decorated with an antibody-binding moiety specific for the fragment crystallizable (Fc) domain can be used a modular system targeted cancer therapy. The Fc-EVs different types immunoglobulin G antibody thus to virtually any tissue interest. Following optimization engineered screening Fc-binding EV-sorting moieties,...
Exosomes released from cells can transfer both functional proteins and RNAs between cells. In this study we tested the hypothesis that muscle might transmit specific signals during lipid-induced insulin resistance through exosomal route.Exosomes were collected quadriceps muscles of C57Bl/6 mice fed for 16 weeks with either a standard chow diet (SD) or an SD enriched 20% palm oil (HP) C2C12 exposed to 0.5 mmol/l palmitate (EXO-Post Palm), oleate Oleate) BSA BSA).HP-fed obese resistant had...
Extracellular vesicles (EVs) play a significant role in cell-cell communication numerous physiological processes and pathological conditions, offer promise as novel biomarkers therapeutic agents for genetic diseases. Many recent studies have described different molecular mechanisms that contribute to EV biogenesis release from cells. However, little is known about how external stimuli such cell culture conditions can affect the quantity content of EVs. While N2a neuroblastoma cells cultured...
Abstract Mesenchymal stromal cells (MSCs) have been investigated as a treatment for various inflammatory diseases because of their immunomodulatory and reparative properties. However, many basic questions concerning mechanisms action after systemic infusion remain unanswered. We performed detailed analysis the properties proteomic profile MSCs systemically administered to two patients with severe refractory acute respiratory distress syndrome (ARDS) on compassionate use basis attempted...
Extracellular vesicles (EV) convey biological information by transmitting macromolecules between cells and tissues are of great promise as pharmaceutical nanocarriers, therapeutic per se. Strategies for customizing the EV surface cargo being developed to enable their tracking, visualization, loading with agents decoration tissue targeting ligands. While much progress has been made in engineering EVs, an exhaustive comparative analysis most commonly exploited EV-associated proteins, well a...
miRNAs are noncoding RNAs representing an important class of gene expression modulators. Extracellular circulating both candidate biomarkers for disease pathogenesis and mediators cell-to-cell communication. We examined the miRNA profile total serum serum-derived exosome-enriched extracellular vesicles in people with normal glucose tolerance or type 2 diabetes. In contrast to miRNA, which did not reveal any differences expression, we identified differentially abundant patients diabetes using...
Extracellular vesicles (EVs) mediate cell-to-cell communication by delivering or displaying macromolecules to their recipient cells. While certain broad-spectrum EV effects reflect protein cargo composition, others have been attributed individual EV-loaded molecules such as specific miRNAs. In this work, we investigated the contents of vesicular using small RNA sequencing cells and EVs from HEK293T, RD4, C2C12, Neuro2a C17.2. The majority content in (49-96%) corresponded rRNA-, coding- tRNA...
We have determined whether orange juice-derived nanovesicles (ONVs) could be used for the treatment of obesity-associated intestinal complications. ONVs were characterized by lipidomic, metabolomic, electron microscopy. In vitro, barriers (IBs = Caco-2+HT-29-MTX) treated with and co-cultured adipocytes to monitor IB fat release. vivo, obesity was induced a high-fat, high-sucrose diet (HFHSD mice) 12 weeks. Then, half HFHSD mice gavaged ONVs. One-month ONV did not modify HFHSD-induced insulin...
The clinical use of chemotherapeutics is limited by several factors, including low cellular uptake, short circulation time, and severe adverse effects. Extracellular vesicles (EVs) have been suggested as a drug delivery platform with the potential to overcome these limitations. EVs are cell-derived, lipid bilayer nanoparticles, important for intercellular communication. They can transport bioactive cargo throughout body, surmount biological barriers, target variety tissues. Several small...
Extracellular vesicles (EVs) have shown promise as potential therapeutics for the treatment of various diseases. However, their rapid clearance after administration could be a limitation in certain therapeutic settings. To solve this, an engineering strategy is employed to decorate albumin onto surface EVs through display binding domains (ABDs). ABDs were either included extracellular loops select EV-enriched tetraspanins (CD63, CD9 and CD81) or directly fused terminal single transmembrane...
Extracellular vesicles (EVs) are gaining ground as next-generation drug delivery modalities. Genetic fusion of the protein interest to a scaffold with high EV-sorting ability represents robust cargo loading strategy. To address paucity such proteins, we leverage simple and reliable assay that can distinguish intravesicular proteins from surface- well non-vesicular compare potential 244 candidate proteins. We identify 24 conserved abilities across five types producer cells. TSPAN2 TSPAN3...
Abstract Messenger RNA (mRNA) has emerged as an attractive therapeutic molecule for a plethora of clinical applications. For in vivo functionality, mRNA therapeutics require encapsulation into effective, stable, and safe delivery systems to protect the cargo from degradation reduce immunogenicity. Here, bioengineering platform efficient loading functional using bionormal nanoparticles, extracellular vesicles (EVs), is established by expressing highly specific RNA‐binding domain fused CD63 EV...
Extracellular vesicles (EVs) are promising tools to transfer macromolecular therapeutic molecules recipient cells, however, efficient functional intracellular protein delivery by EVs remains challenging. Here, we have developed novel and versatile systems that leverage selected molecular engineer for robust cytosolic both in vitro vivo. These systems, termed VSV-G plus EV-sorting Domain-Intein-Cargo (VEDIC) VSV-G-Foldon-Intein-Cargo (VFIC), exploit an engineered mini-intein (intein) with...