A5038756734- Microtubule and mitosis dynamics
- Protist diversity and phylogeny
- Genetic and Kidney Cyst Diseases
- Advanced Fluorescence Microscopy Techniques
- Extracellular vesicles in disease
- RNA Interference and Gene Delivery
- Cellular Mechanics and Interactions
- Advanced Electron Microscopy Techniques and Applications
- Photosynthetic Processes and Mechanisms
- Nuclear Structure and Function
- Photoreceptor and optogenetics research
- Advanced NMR Techniques and Applications
- Lipid Membrane Structure and Behavior
- Cell Adhesion Molecules Research
- RNA Research and Splicing
Imperial College London
2022-2023
The London College
2023
University of Geneva
2017-2020
Novartis (Switzerland)
2016-2019
Novartis Institutes for BioMedical Research
2016-2019
Exosomes are nanovesicles released by virtually all cells, which act as intercellular messengers transfer of protein, lipid, and RNA cargo. Their quantitative efficiency, routes cell uptake, subcellular fate within recipient cells remain elusive. We quantitatively characterize exosome saturates with dose time reaches near 100% transduction efficiency at picomolar concentrations. Highly reminiscent pathogenic bacteria viruses, exosomes recruited single vesicles to the body surfing on...
Extracellular vesicles (EV) convey biological information by transmitting macromolecules between cells and tissues are of great promise as pharmaceutical nanocarriers, therapeutic per se. Strategies for customizing the EV surface cargo being developed to enable their tracking, visualization, loading with agents decoration tissue targeting ligands. While much progress has been made in engineering EVs, an exhaustive comparative analysis most commonly exploited EV-associated proteins, well a...
Centrioles are microtubule-based organelles responsible for forming centrosomes and cilia, which serve as microtubule-organizing, signaling, motility centers. Biogenesis maintenance of centrioles with proper number, size, architecture vital their functions during development physiology. While centriole number control has been well-studied, less is understood about stable structures conserved size cell division ciliary motility. Here, we identified CCDC15 a protein that colocalizes interacts...
Centrioles are evolutionarily conserved macromolecular structures that fundamental to form cilia, flagella, and centrosomes. 9-fold symmetrical microtubule-based cylindrical barrels comprising three regions can be clearly distinguished in the Chlamydomonas reinhardtii organelle: an ∼100-nm-long proximal region harboring a cartwheel; ∼250-nm-long central core containing Y-shaped linker; ∼150-nm-long distal ending at transitional plate. Despite discovery of many centriolar components, no...
Centrioles are characterized by a nine-fold arrangement of microtubule triplets held together an inner protein scaffold. These structurally robust organelles experience strenuous cellular processes such as cell division or ciliary beating while performing their function. However, the molecular mechanisms underlying stability triplets, well centriole architectural integrity remain poorly understood. Here, using ultrastructure expansion microscopy for nanoscale mapping, we reveal that POC16...
Assembly of the ciliary microtubule doublet The cilium is a conserved organelle that crucial for motility as well sensing extracellular environment. Its core structure characterized by nine doublets (MTDs). mechanisms MTD assembly are unclear. Schmidt-Cernohorska et al. developed an assay to reconstitute in vitro. Tubulin carboxyl-terminal tails played critical inhibitory role formation. Molecular dynamics revealed A11 protofilament regulated initiation. Furthermore, live-cell imaging showed...
Super-resolution microscopy reveals the molecular organization of biological structures down to nanoscale. While it allows study protein complexes in single cells, small organisms, or thin tissue sections, there is currently no versatile approach for ultrastructural analysis compatible with whole vertebrate embryos. Here, we present ultrastructure expansion (TissUExM), a method expand millimeter-scale and mechanically heterogeneous embryonic tissues, including
Summary Centrioles are characterized by a nine-fold arrangement of long-lived microtubule triplets that held together an inner protein scaffold. These structurally robust organelles experience strenuous cellular processes such as cell division or ciliary beating while performing their function. However, the molecular mechanisms underlying stability triplets, well centriole architectural integrity remain poorly understood. Here, using ultrastructure expansion microscopy (U-ExM) for nanoscale...
Expansion microscopy of millimeter-large mechanically heterogeneous tissues, such as whole vertebrate embryos, has been limited, particularly when combined with post-expansion immunofluorescence. Here, we present a protocol to perform ultrastructure expansion optimized labeling. We describe steps for embedding and denaturing zebrafish larvae or mouse embryos. then detail procedures hydrogel handling mounting. This is well suited super-resolution imaging macromolecular protein complexes in...
Abstract Centrioles are evolutionarily conserved microtubule-based organelles critical to form centrosomes and cilia, which act as microtubule-organizing, signaling motility centers. Biogenesis maintenance of centrioles with proper number, size architecture crucial for their functions during development physiology. Consequently, deregulation causes developmental disorders cancer. Although centriole number control has been extensively studied, less is known about how maintained stable...