A5002105711- Microtubule and mitosis dynamics
- Genetic and Kidney Cyst Diseases
- Advanced Fluorescence Microscopy Techniques
- Chromosomal and Genetic Variations
- Genetic Syndromes and Imprinting
- Genomics and Chromatin Dynamics
- 14-3-3 protein interactions
- Advanced Electron Microscopy Techniques and Applications
- Renal and related cancers
- Protist diversity and phylogeny
- Parasitic Infections and Diagnostics
- Genetics and Neurodevelopmental Disorders
- Estrogen and related hormone effects
- Photosynthetic Processes and Mechanisms
- Plant Reproductive Biology
- Cancer therapeutics and mechanisms
- Protein Kinase Regulation and GTPase Signaling
- Telomeres, Telomerase, and Senescence
- Prenatal Screening and Diagnostics
- Skin Protection and Aging
- Mast cells and histamine
- Cancer, Hypoxia, and Metabolism
- Plant Molecular Biology Research
- Vector-borne infectious diseases
- Near-Field Optical Microscopy
Max Perutz Labs
2023-2024
Vienna Biocenter
2023-2024
Czech Academy of Sciences, Institute of Molecular Genetics
2012-2024
University of Vienna
2023-2024
Czech Academy of Sciences
2017-2021
University of Geneva
2018-2019
Institute of Molecular Genetics
2019
Charles University
2015
Retrotransposons are “copy-and-paste” insertional mutagens that substantially contribute to mammalian genome content. often carry long terminal repeats (LTRs) for retrovirus-like reverse transcription and integration into the genome. We report an extraordinary impact of a group LTRs from endogenous retrovirus-related ERVL retrotransposon class on gene expression in germline beyond. In mouse, we identified more than 800 ORR1, MT, MT2, MLT families, which resemble mobile gene-remodeling...
Assembly of the ciliary microtubule doublet The cilium is a conserved organelle that crucial for motility as well sensing extracellular environment. Its core structure characterized by nine doublets (MTDs). mechanisms MTD assembly are unclear. Schmidt-Cernohorska et al. developed an assay to reconstitute in vitro. Tubulin carboxyl-terminal tails played critical inhibitory role formation. Molecular dynamics revealed A11 protofilament regulated initiation. Furthermore, live-cell imaging showed...
Expansion microscopy (ExM) has become a powerful super-resolution method in cell biology. It is simple, yet robust approach, which does not require any instrumentation or reagents beyond those present standard facility. In this study, we used kinetoplastid parasites Trypanosoma brucei and Leishmania major , possess complex, well-defined microtubule-based cytoskeleton, to demonstrate that recapitulates faithfully morphology of structures as previously revealed by combination sophisticated...
γ-Tubulin is the key protein for microtubule nucleation. Duplication of γ-tubulin gene occurred several times during evolution, and in mammals genes encode proteins which share ∼97% sequence identity. Previous analysis Tubg1 Tubg2 knock-out mice has suggested that γ-tubulins are not functionally equivalent. died at blastocyst stage, whereas developed normally were fertile. It was proposed 1 represents ubiquitous γ-tubulin, while 2 may have some specific functions cannot substitute deficiency...
Abstract Expansion microscopy (ExM) is a method to magnify physically specimen with preserved ultrastructure. It has the potential explore structural features beyond diffraction limit of light. The procedure been successfully used for different animal species, from isolated macromolecular complexes through cells tissue slices. plant-derived samples still at beginning, and little known, whether chromatin ultrastructure becomes altered by physical expansion. In this study, we expanded barley...
Centrioles are highly elaborate microtubule-based structures responsible for the formation of centrosomes and cilia. Despite considerable variation across species tissues within any given tissue, their size is essentially constant [1, 2]. While diameter centriole cylinder set by dimensions inner scaffolding structure cartwheel [3], how length so precisely stably maintained over many cell divisions not well understood. Cep97 CP110 conserved proteins that localize to distal end centrioles have...
Bardet–Biedl syndrome (BBS) is a pleiotropic ciliopathy caused by dysfunction of primary cilia. More than half BBS patients carry mutations in one eight genes encoding for subunits protein complex, the BBSome, which mediates trafficking ciliary cargoes. In this study, we elucidated mechanisms BBSome assembly living cells and how process spatially regulated. We generated large library human cell lines deficient particular subunit expressing another tagged with fluorescent protein. analyzed...
Abstract Ag-mediated activation of mast cells initiates signaling events leading to Ca2+ response, release allergic mediators from cytoplasmic granules, and synthesis cytokines chemokines. Although microtubule rearrangement during has been described, the molecular mechanisms that control their remodeling are largely unknown. Microtubule nucleation is mediated by complexes formed γ-tubulin complex proteins. In this study, we report that, in bone marrow–derived (BMMCs), interacts with...
ABSTRACT For decades, electron microscopy (EM) was the only method able to reveal ultrastructure of cellular organelles and molecular complexes because diffraction limit optical microscopy. In recent past, emergence superresolution fluorescence enabled visualization structures with so far unmatched spatial resolution approaching virtually dimensions. Despite these technological advances, currently super-resolution does not permit same level as provided by microscopy, impeding attribution a...
SUMMARY Centrioles are highly elaborate microtubule-based structures responsible for the formation of centrosomes and cilia. Despite considerable variation across species tissues, within any given tissue their size is essentially constant [1, 2]. While diameter centriole cylinder set by dimensions inner scaffolding structure cartwheel [3], how length so precisely stably maintained over many cell divisions not well understood. Cep97 CP110 conserved proteins that localize to distal end...
Abstract Expansion Microscopy (ExM) is a method to magnify physically specimen with preserved ultrastructure. It has the potential explore structural features beyond diffraction limit of light. The procedure been successfully used for different animal species, from isolated macromolecular complexes through cells tissue slices. plant-derived samples still at beginning, and little known whether chromatin ultrastructure becomes altered by physical expansion. In this study, we expanded barley...
Abstract Bardet-Biedl Syndrome (BBS) is a pleiotropic ciliopathy caused by dysfunction of primary cilia. Most BBS patients carry mutations in one eight genes encoding for subunits protein complex, BBSome, which mediates the trafficking ciliary cargoes. Although, structure BBSome has been resolved recently, mechanism assembly this complicated complex living cells poorly understood. We generated large library human retinal epithelial cell lines deficient particular subunit and expressing...
Abstract Bardet-Biedl syndrome (BBS) is a pleiotropic ciliopathy caused by dysfunction of the BBSome, cargo adaptor essential for export transmembrane receptors from cilia. Although actin-dependent ectocytosis has been proposed as compensatory cellular process defective retrieval, underlying molecular mechanism poorly understood, particularly in context BBS pathology. In this study, we investigated how actin polymerization and are regulated within cilium. Our findings reveal that ciliary...