A5049739233- Microtubule and mitosis dynamics
- Mast cells and histamine
- Photosynthetic Processes and Mechanisms
- 14-3-3 protein interactions
- Ubiquitin and proteasome pathways
- Protist diversity and phylogeny
- Cellular transport and secretion
- Polyamine Metabolism and Applications
- Monoclonal and Polyclonal Antibodies Research
- PI3K/AKT/mTOR signaling in cancer
- DNA Repair Mechanisms
- Glioma Diagnosis and Treatment
- Nuclear Structure and Function
- Genomics and Chromatin Dynamics
- Cellular Mechanics and Interactions
- Glycosylation and Glycoproteins Research
- RNA Research and Splicing
- Plant Molecular Biology Research
- Fungal and yeast genetics research
- Click Chemistry and Applications
- Epigenetics and DNA Methylation
- Protein Tyrosine Phosphatases
- Cancer-related Molecular Pathways
- Hair Growth and Disorders
- Photoreceptor and optogenetics research
Czech Academy of Sciences
2005-2025
Czech Academy of Sciences, Institute of Molecular Genetics
2015-2025
Institute of Molecular Genetics
2014
Mast cell activation mediated by the high affinity receptor for IgE (FcεRI) is a key event in allergic response and inflammation. Other receptors on mast cells, as c-Kit stem factor G protein-coupled (GPCRs) synergistically enhance FcεRI-mediated release of inflammatory mediators. Activation various signaling pathways cells results changes morphology, adhesion to substrate, exocytosis, migration. Reorganization cytoskeleton pivotal all these processes. Cytoskeletal proteins also play an...
γ-Tubulin is assumed to participate in microtubule nucleation acentrosomal plant cells, but the underlying molecular mechanisms are still unknown. Here, we show that γ-tubulin present protein complexes of various sizes and different subcellular locations Arabidopsis fava bean. Immunoprecipitation experiments revealed an association with αβ-tubulin dimers. cosedimented microtubules polymerized vitro localized along their whole length. Large resistant salt treatment were found be associated a...
Profilin controls actin nucleation and assembly processes in eukaryotic cells. Actin elongation promoting factors (NEPFs) such as Ena/VASP, formins, WASP-family proteins recruit profilin:actin for filament formation. Some of these are found to be microtubule associated, making polymerization from microtubule-associated platforms possible. Microtubules implicated focal adhesion turnover, cell polarity establishment, migration, illustrating the coupling between systems. Here we demonstrate...
Abstract Aggregation of the high-affinity IgE receptors (FcεRIs) on surface granulated mast cells initiates a chain signaling events culminating in release allergy mediators. Although microtubules are involved cell degranulation, molecular mechanism that controls microtubule rearrangement after FcεRI triggering is poorly understood. In this study, we show activation bone marrow-derived (BMMCs) induced by aggregation or treatment with pervanadate leads to rapid polymerization microtubules....
Abstract γ‐Tubulin is assumed to be a typical cytosolic protein necessary for nucleation of microtubules from microtubule organizing centers. Using immunolocalization and cell fractionation techniques in combination with siRNAi expression FLAG‐tagged constructs, we have obtained evidence that γ‐tubulin also present nucleoli mammalian interphase cells diverse cellular origins. Immunoelectron microscopy has revealed localization outside fibrillar centers where transcription ribosomal DNA takes...
Tubulin self-assembly into microtubules is a fascinating natural phenomenon. Its importance not just crucial for functional and structural biological processes, but it also serves as an inspiration synthetic nanomaterial innovations. The modulation of the tubulin process without introducing additional chemical inhibitors/promoters or stabilizers has remained elusive process. This work reports versatile vigorous strategy controlling by nanosecond electropulses (nsEPs). polymerization assessed...
Abstract Background G protein-coupled receptor kinase-interacting proteins (GITs) function as GTPase-activating (GAPs) for small GTPases of the ADP-ribosylation factor (Arf) family. While GIT (GIT1 and GIT2) regulate both cell migration microtubule organization, their corresponding regulatory mechanisms in glioblastoma cells remain largely unknown. To further investigate role modulation, we examined GITs nucleation involvement protein kinase C (PKC) this process. Methods Glioblastoma lines...
The molecular mechanisms controlling microtubule formation in cells with non-centrosomal microtubular arrays are not yet fully understood. key component of nucleation is γ-tubulin. Although previous results suggested that tyrosine kinases might serve as regulators γ-tubulin function, their exact roles remain enigmatic. In the present study, we show a pool associates detergent-resistant membranes differentiating P19 embryonal carcinoma cells, which exhibit elevated expression Src family...
γ-Tubulin is the key protein for microtubule nucleation. Duplication of γ-tubulin gene occurred several times during evolution, and in mammals genes encode proteins which share ∼97% sequence identity. Previous analysis Tubg1 Tubg2 knock-out mice has suggested that γ-tubulins are not functionally equivalent. died at blastocyst stage, whereas developed normally were fertile. It was proposed 1 represents ubiquitous γ-tubulin, while 2 may have some specific functions cannot substitute deficiency...
γ-Tubulins are highly conserved members of the tubulin superfamily essential for microtubule nucleation. Humans possess 2 γ-tubulin genes. It is thought that γ-tubulin-1 represents a ubiquitous isotype, whereas γ-tubulin-2 found predominantly in brain, where it may be endowed with divergent functions beyond The molecular basis purported functional differences between γ-tubulins unknown. We report discrimination human according to their electrophoretic and immunochemical properties. In vitro...
The expression, cellular distribution, and subcellular sorting of the microtubule (MT)-nucleating γ-tubulin small complex (γTuSC) proteins, GCP2 GCP3, were studied in human glioblastoma cell lines clinical tissue samples representing all histologic grades adult diffuse astrocytic gliomas (n = 54). Quantitative real-time polymerase chain reaction revealed a significant increase expression GCP3 transcripts cells versus normal astrocytes; these associated with higher amounts both γTuSC...
At the end of growth phase, mouse antral follicle oocytes acquire full developmental competence. In mouse, this event is marked by transition from so-called non-surrounded nucleolus (NSN) chromatin configuration into transcriptionally quiescent surrounded (SN) configuration, which named after a prominent perinucleolar condensed ring. However, SN alone not sufficient for determining competence oocyte. There are additional nuclear and cytoplamic factors involved, while little known about...
Abstract Ag-mediated activation of mast cells initiates signaling events leading to Ca2+ response, release allergic mediators from cytoplasmic granules, and synthesis cytokines chemokines. Although microtubule rearrangement during has been described, the molecular mechanisms that control their remodeling are largely unknown. Microtubule nucleation is mediated by complexes formed γ-tubulin complex proteins. In this study, we report that, in bone marrow–derived (BMMCs), interacts with...
Abstract De novo heterozygous missense variants in the γ-tubulin gene TUBG1 have been linked to human malformations of cortical development associated with intellectual disability and epilepsy. Here, we investigated through in-utero electroporation in-vivo studies, how four these affect development. We show that mutants neuronal positioning, disrupting locomotion new-born neurons but without affecting progenitors’ proliferation. further demonstrate pathogenic are reduced microtubule dynamics...
gamma-Tubulin is necessary for nucleation and polar orientation of microtubules in vivo. The molecular mechanism microtubule by gamma-tubulin the regulation this process are not fully understood. Here we show that there two forms brain present complexes various sizes. Large tend to dissociate presence a high salt concentration. Both gamma-tubulins co-polymerized with tubulin dimers, multiple bands were identified protein preparations under conditions non-denaturing electrophoresis....
Abstract Background The function of the cortical microtubules, composed αβ-tubulin heterodimers, is linked to their organizational state which subject spatial and temporal modulation by environmental cues. role tubulin posttranslational modifications in these processes largely unknown. Although antibodies against small regions represent useful tool for studying molecular configuration data on exposure epitopes plant microtubules are still limited. Results Using homology modeling we have...
Profilin 1 is a crucial actin regulator, interacting with monomeric and several actin-binding proteins controlling polymerization. Recently, it has become evident that this profilin isoform associates microtubules via formins interferes microtubule elongation at the cell periphery. Recruitment of microtubule-associated upon extensive polymerizations, for example, edge, enhances growth, indicating contributes to coordination organization. Here, we provide further evidence profilin-microtubule...
ER distribution depends on microtubules, and homeostasis disturbance activates the unfolded protein response resulting in remodeling. CDK5RAP3 (C53) implicated various signaling pathways interacts with UFM1-protein ligase 1 (UFL1), which mediates ufmylation of proteins to stress. Here we find that UFL1 C53 associate γ-tubulin ring complex proteins. Knockout or human osteosarcoma cells induces stress boosts centrosomal microtubule nucleation accompanied by accumulation, formation, expansion....
The antigen-mediated activation of mast cells initiates signaling events leading to their degranulation, the release inflammatory mediators, and synthesis cytokines chemokines. Although rapid transient microtubule reorganization during has been described, molecular mechanisms that control rearrangement are largely unknown. Microtubule nucleation is mediated by γ-tubulin complexes. In this study, we report on regulation in bone marrow-derived (BMMCs) Src homology 2 (SH2) domain-containing...