A5102885031- Cellular Mechanics and Interactions
- Cardiomyopathy and Myosin Studies
- Reproductive tract infections research
- Cell Adhesion Molecules Research
- Microtubule and mitosis dynamics
- Heat shock proteins research
- Muscle Physiology and Disorders
- Cervical Cancer and HPV Research
- 3D Printing in Biomedical Research
- Nuclear Structure and Function
- Force Microscopy Techniques and Applications
- Cell Image Analysis Techniques
- RNA Research and Splicing
- Adolescent Sexual and Reproductive Health
- Advanced Fluorescence Microscopy Techniques
- Skin and Cellular Biology Research
- Molecular Biology Techniques and Applications
- Enzyme Structure and Function
- Hormonal Regulation and Hypertension
- Thyroid Disorders and Treatments
- Polymer Surface Interaction Studies
- Urinary Tract Infections Management
- Protein Structure and Dynamics
- Pelvic floor disorders treatments
- Social and Educational Sciences
Stockholm University
2014-2023
Wenner-Gren Foundations
1999-2022
Umeå University
1992-2020
Trinity House
2013
Karolinska University Hospital
1994-2002
Karolinska Institutet
2002
Princeton University
1998
Lund University
1995
Uppsala University Hospital
1995
Uppsala University
1980-1993
The prevalence of human papillomavirus (HPV) infection in cervical cell scrapes from a cohort 276 young women was determined by general two-step polymerase chain reaction. HPV fluctuated among during 2-year interval. total decreased 21%to 8.3%. most prevalent types at enrollment were HPV16 (3.3%) and HPV-6 (2.9%). At follow-up, the common type HPV-16 (2.9%), while no detected. In 2 only, same persisted. Regression found 80% women. A new type-specific detected 7.2% independently associated...
Abstract Vesicle fusion is executed via formation of an Ω-shaped structure (Ω-profile), followed by closure (kiss-and-run) or merging the Ω-profile into plasma membrane (full fusion). Although limits release but recycles vesicles economically, facilitates couples to classical endocytosis for recycling. Despite its crucial role in determining exocytosis/endocytosis modes, how mediated poorly understood endocrine cells and neurons containing small ∼30–300 nm vesicles. Here, using confocal...
The prevalence of human papillomavirus (HPV) infection in cervical cell scrapes from young women was determined by polymerase chain reaction (PCR) using general primer pairs localized within the L1 region. With a one-step PCR, 5.9% (35 590) population-based study were found to contain HPV DNA. proportion HPV-positive increased with age, 1.4% (1 69) among aged 19 years 9.2% (13 142) 25 years. Among normal cytology, 5.6% (30 539) harbored A total 5 (26.3%) pathological signs positive for By...
Skeletal muscle weakness in patients suffering from rheumatoid arthritis (RA) adds to their impaired working abilities and reduced quality of life. However, little molecular insight is available on associated with RA. Oxidative stress has been implicated the disease pathogenesis Here we show that oxidative post-translational modifications contractile machinery targeted actin result polymerization force production. Using mass spectrometry, identified residues by 3-nitrotyrosine (3-NT) or...
Background and Objectives. Previous studies of relationships between genital human papillomavirus infection tentative risk factors have yielded conflicting results, possibly because inaccuracy the viral detection methods used differences in selection criteria. Goal This Study. To determine prevalence identify a group young Swedish women. Study Design. was population-based study involving comu-pletion structured questionnaire, analysis cervical scrapings for Chlamydia trachomatis, sero-logic...
The actin-binding protein, profilin, contains a src-homology (SH) 3-like fold (Schutt, C.E. et al., submitted), and its tight interaction with poly(L-proline) is reminiscent of the binding activity exhibited by SH3-domains. Here we demonstrate that replacements aromatic amino acids in hydrophobic patch on surface profilin molecule abolish poly(L-proline)-binding capacity. However, location this found another region than displaying structural similarities SH3 domains.
Profilin controls actin nucleation and assembly processes in eukaryotic cells. Actin elongation promoting factors (NEPFs) such as Ena/VASP, formins, WASP-family proteins recruit profilin:actin for filament formation. Some of these are found to be microtubule associated, making polymerization from microtubule-associated platforms possible. Microtubules implicated focal adhesion turnover, cell polarity establishment, migration, illustrating the coupling between systems. Here we demonstrate...
Properties of human profilin I mutated in the major actin-binding site were studied and compared with wild-type using β/γ-actin as interaction partner. The mutants ranged affinity, from those that only weakly affected polymerization actin to one bound more strongly than profilin. With profilins, whose sequestering activity was low, concentration free monomers observed at steady-state [Afree], close seen alone ([Acc], critical polymerization). Profilin binding an intermediate affinity like...
<b>Objectives:</b> To measure the coverage, gender distribution, and participants' prevalence of genital <i>Chlamydia trachomatis</i> infections using a new internet based self selective testing approach by means home sampling in general population Sweden. investigate factors associated with these measures. <b>Methods:</b> Observational survey people actively ordering coded packages, checking their test results, from known website. No personal invitations were sent out. All inhabitants (256...
The binding of phosphatidylinositol(4,5)‐bisphosphate (PI(4,5)P 2 ) to profilin at a region distinct from the actin interaction surface is demonstrated by experiments with covalently cross‐linked profilin:β‐actin. result in agreement observations made several mutant profilins and provides strong evidence for two regions on mammalian mediating electrostatic phosphatidylinositol lipids; one close site poly( L ‐proline), partially overlapping actin‐binding surface. Congruent this, plant...
Palladin is an actin‐associated protein that has been suggested to play critical roles in establishing cell morphology and maintaining cytoskeletal organization a wide variety of types. shown previously bind directly three different actin‐binding proteins vasodilator‐stimulated phosphoprotein (VASP), α‐actinin ezrin, suggesting it functions as organizing unit recruits actin‐regulatory specific subcellular sites. contains sequences resembling motif known profilin. Here, we demonstrate...
Chaperonin containing tailless complex polypeptide 1 (CCT) forms a classical chaperonin barrel structure where two rings of subunits surround central cavity. Each ring consists eight distinct subunits, creating binding interface that makes CCT unique among the chaperonins. In addition to acting as multimeric chaperonin, there is increasing evidence indicating when monomeric, possess additional functions. Here we assess role individually, using GFP (green fluorescent protein) tagging approach...
Actin is one of the proteins that rely on chaperonins for proper folding. This paper shows thermal unfolding G‐actin, as studied by CD and ultraviolet difference spectrometry, coincides with a loss in DNase I‐inhibiting activity protein. Thus, I inhibition assay should be useful systematic studies actin refolding. Using this assay, we have investigated how stability affected either Ca 2 + or Mg at high affinity divalent cation binding site, concentration excess nucleotide, nucleotide...
The aim of this study was to determine the associations between risk behaviour and women's reported sexually transmitted diseases (STDs). All women aged 19, 21, 23 25, residing in a specified housing area, were invited answer questionnaire regarding their sexual behaviour, smoking alcohol consumption previous history STD. Of 611 participating, one out 4 had at least In an univariate analysis, self-reported STD found be related age, having more than lifetime partners, practised intercourse...
Proliferation and fusion of myoblasts are needed for the generation repair multinucleated skeletal muscle fibers in vivo. Studies myocyte differentiation, cell fusion, limited by an appropriate vitro culture system. We developed a novel technique [two-dimensional syncytia (2DMS) technique] that results formation myotubes, organized parallel much like arrangement tissue. This is based on UV lithography-produced micro-patterned glass which conventionally cultured C2C12 proliferate, align, fuse...
Correct protein folding is fundamental for maintaining homeostasis and avoiding the formation of potentially cytotoxic aggregates. Although some proteins appear to fold unaided, actin requires assistance from oligomeric molecular chaperone CCT. Here we report an additional connection between CCT by identifying one subunits, CCTε, as a component myocardin-related cotranscription factor-A (MRTF-A)/serum response factor (SRF) pathway. The SRF pathway registers changes in G-actin levels, leading...
Abstract Platelets respond to stimulatory agents in general by the formation of long spikelike surface projections built up tightly bundled microfilaments. During contact stimulation this is followed a second phase when thin membrane lamellae grow out between projections. This behaviour resembles that seen for instance fibroblasts and glial cells, sending microspikes lamellipodia as step their advancement over solid substrata. Conditions, designed earlier preservation visualization fragile...