A5088317241- Cardiomyopathy and Myosin Studies
- Force Microscopy Techniques and Applications
- Cellular Mechanics and Interactions
- Muscle Physiology and Disorders
- Polymer Surface Interaction Studies
- Heat shock proteins research
- Molecular Junctions and Nanostructures
- Enzyme Structure and Function
- Micro and Nano Robotics
- Enzyme function and inhibition
- Neurotransmitter Receptor Influence on Behavior
- Microfluidic and Bio-sensing Technologies
- Muscle activation and electromyography studies
- Protein Structure and Dynamics
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Hemoglobin structure and function
- Cardiovascular Effects of Exercise
- Genetic Neurodegenerative Diseases
- Microtubule and mitosis dynamics
- Phosphorus and nutrient management
- Electron Spin Resonance Studies
- Nanofabrication and Lithography Techniques
- Monoclonal and Polyclonal Antibodies Research
- Aldose Reductase and Taurine
- Dendrimers and Hyperbranched Polymers
National Food Administration
2023
Karolinska Institutet
1996-2020
Karolinska University Hospital
2007-2020
Linnaeus University
2010-2019
McGill University
2017-2019
Cargotec (Poland)
2015
Linköping University
1995-2007
Umeå University
1998-2007
Norwegian University of Science and Technology
2007
Uppsala University
2007
Significance Electronic computers are extremely powerful at performing a high number of operations very speeds, sequentially. However, they struggle with combinatorial tasks that can be solved faster if many performed in parallel. Here, we present proof-of-concept parallel computer by solving the specific instance {2, 5, 9} classical nondeterministic-polynomial-time complete (“NP-complete”) problem, subset sum problem. The consists specifically designed, nanostructured network explored large...
Three different simulation tools were used to simulate a low energy terraced house in the south of Sweden. The software all use dynamic models calculate, for example, demand heating and indoor temperatures. aim this paper is discuss relative importance annual aspects Swedish low-energy house. Both measured simulated values are considered compared. focus on impact choice software, habits tenants, design parameters such as ventilation rates. total electricity range from about 6000 kWh over 12...
Skeletal muscle weakness in patients suffering from rheumatoid arthritis (RA) adds to their impaired working abilities and reduced quality of life. However, little molecular insight is available on associated with RA. Oxidative stress has been implicated the disease pathogenesis Here we show that oxidative post-translational modifications contractile machinery targeted actin result polymerization force production. Using mass spectrometry, identified residues by 3-nitrotyrosine (3-NT) or...
Serotonergic neurotransmission has been implicated in suicidal behavior. Polymorphisms the genes coding for tryptophan hydroxylase, serotonin receptor 2A and transporter were investigated a sample of suicide attempters (n = 165) healthy control subjects 99). No significant differences found any polymorphisms. Neither did emerge comparison with when grouped into different diagnostic categories: unipolar disorder 45), adjustment 37), substance use 37) personality disorder, cluster B 36). The...
Portable biosensor systems would benefit from reduced dependency on external power supplies as well further miniaturization and increased detection rate. Systems built around self-propelled biological molecular motors cytoskeletal filaments hold significant promise in these regards they are nanoscale components that enable nanoseparation independent of fluidic pumping. Previously reported microtubule-kinesin based devices slow, however, compared to several existing systems. Here we...
The mechanisms that modulate the kinetics of muscle relaxation are critically important for function. A prime example impact impaired is nemaline myopathy caused by mutations in KBTBD13 (NEM6). In addition to weakness, NEM6 patients have slow relaxation, compromising contractility and daily life activities. role unknown, pathomechanism underlying undetermined. combination transcranial magnetic stimulation-induced fiber- sarcomere-contractility assays, low-angle x-ray diffraction,...
In the in vitro motility assay, actin filaments are propelled by surface-adsorbed myosin motors, or rather, motor fragments such as heavy meromyosin (HMM). Recently, efforts have been made to develop actomyosin powered nanodevices on basis of this assay but developments hampered limited understanding HMM adsorption geometry. Therefore, we here investigate geometries trimethylchlorosilane- [TMCS-] derivatized hydrophobic surfaces and hydrophilic negatively charged (SiO2). The TMCS surface is...
In this paper, an LC-MS/MS method for the simultaneous identification and quantification of cyanotoxins with hydrophilic lipophilic properties in edible bivalves is presented. The includes 17 comprising 13 microcystins (MCs), nodularin (NOD), anatoxin-a (ATX-a), homoanatoxin (h-ATX) cylindrospermopsin (CYN). A benefit to presented possibility MS detection MC-LR-[Dha7] MC-LR-[Asp3] as separately identified MS-resolved MRM signals, two congeners which were earlier detected together....
Abstract. Objectives. We asked if single nucleotide polymorphisms (SNP) in inflammatory cytokine genes related to 3‐year survival ill elderly subjects and genotypes differed between the a younger control population. Design. Prospective observational study. Setting. Two geriatric departments at university hospital. Subjects. Eighty three acutely admitted patients (83 ± 7 year, 70% women) 207 young healthy (40 1 37% were included. Outcome measures. Single of tumour necrosis factor (TNF) ‐ α...
Generation of force and movement by actomyosin cross-bridges is the molecular basis muscle contraction, but generally accepted ideas about cross-bridge properties have recently been questioned. Of utmost significance, evidence for nonlinear elasticity has presented. We here investigate how this other newly discovered or postulated phenomena would modify operation, with focus on post-power-stroke events. First, as an experimental basis, we present a hyperbolic [MgATP]-velocity relationship...
Myosin II propelled actin filaments move ten times faster than kinesin driven microtubules and are thus attractive candidates as cargo-transporting shuttles in motor lab-on-a-chip devices. In addition, actomyosin-based transportation of nanoparticles is useful various fundamental studies. However, it poorly understood how actomyosin function affected by different number nanoscale cargoes, cargo size, the mode cargo-attachment to filament. This studied here using biotin/fluorophores,...
Objective Nemaline myopathy (NM) is one of the most common congenital nondystrophic myopathies and characterized by muscle weakness, often from birth. Mutations in ACTA1 are a frequent cause NM (ie, NEM3). encodes alpha‐actin 1, main constituent sarcomeric thin filament. The mechanisms which mutations contribute to weakness NEM3 incompletely understood. We hypothesized that dysfunction contributes patients. Methods To test this hypothesis, we performed contractility measurements individual...
High-speed atomic force microscopy (HS-AFM) can be used to study dynamic processes with real-time imaging of molecules within 1- 5-nm spatial resolution. In the current study, we evaluated 3-state model activation cardiac thin filaments (cTFs) isolated as a complex and deposited on mica-supported lipid bilayer. We studied this for conformational changes 1) at low high [Ca
Acceptance and commitment therapy (ACT) is an evidence-based treatment to improve functioning quality of life (QoL) for chronic pain patients, but outreach this unsatisfactory. Internet-delivery has been shown increase access there limited evidence regarding feasibility effectiveness web-based ACT pain. The aim the study was evaluate iterate a novel internet-delivered program, iACT, in clinical self-referred sample patients. intervention developed close collaboration with To enhance...
Tetrodotoxin (TTX), earlier known as a tropical paralytic neurotoxin from the pufferfish poisonings, has increasingly been occurring in edible marine species, including filter-feeding bivalves, relatively cold waters of some European countries. The defined conditions that promote production TTX, its origin or processes accumulation seafood are still not clarified. Recent studies temperate show, however, quantifiable levels TTX bivalves appear to be influenced by seawater temperature...
Lab-on-a-chip systems with molecular motor driven transport of analytes attached to cytoskeletal filament shuttles (actin filaments, microtubules) circumvent challenges nanoscale liquid transport. However, the filaments have limited cargo-carrying capacity and limitations either in transportation speed (microtubules) or control over motility direction (actin). To overcome these constraints we here report incorporation covalently antibodies into self-propelled actin bundles (nanocarriers)...
Biochemical studies in solution and with myosin motor fragments adsorbed to surfaces (in vitro motility assays) are invaluable for elucidation of actomyosin function. However, there is limited understanding how surface adsorption affects properties, e.g., catalytic activity. Here we address this issue by comparing the activity heavy meromyosin (HMM) standard assay [derivatized trimethylchlorosilane (TMCS)]. For these first characterized interaction HMM fluorescent ATP analogue adenosine...
Biosensors would benefit from further miniaturization, increased detection rate and independence external pumps other bulky equipment. Whereas transportation systems built around molecular motors cytoskeletal filaments hold significant promise in the latter regard, recent proof-of-principle devices based on microtubule-kinesin motor system have not matched speed of existing methods. An attractive solution to overcome this limitation be use myosin driven propulsion actin which offers motility...
Human carbonic anhydrase II (HCA II) interacts weakly with GroEL at room temperature. To further investigate this interaction we used electron paramagnetic resonance (EPR) spectroscopy to study HCA cysteine mutants spin-labeled selected positions. From our results it is evident that protein−protein interactions can be specifically mapped by site-directed spin-labeling and EPR measurements. needs unfolded about the same extent as a GuHCl-induced molten-globule intermediate of enzyme interact...
Nine single-cysteine mutants were labeled with 5-(2-iodoacetylaminoethylamino)naphthalene-1-sulfonic acid, an efficient acceptor of Trp fluorescence in resonance energy transfer. The ratio between the intensity 5-(2-acetylaminoethylamino)naphthalene-1-sulfonic acid (AEDANS) moiety excited at 295 nm (Trp absorption) and 350 (direct AEDANS was used to estimate average distances seven residues human carbonic anhydrase II (HCA II) label. Guanidine HCl denaturation HCA variants also performed...