A5030054574- Cancer Immunotherapy and Biomarkers
- Immune cells in cancer
- Brain Metastases and Treatment
- Chemokine receptors and signaling
- Radiopharmaceutical Chemistry and Applications
- Intracranial Aneurysms: Treatment and Complications
- Immunotherapy and Immune Responses
- Lung Cancer Research Studies
- Traumatic Brain Injury and Neurovascular Disturbances
- Axon Guidance and Neuronal Signaling
- Pituitary Gland Disorders and Treatments
- Lung Cancer Treatments and Mutations
- interferon and immune responses
- Hippo pathway signaling and YAP/TAZ
- Glioma Diagnosis and Treatment
- Management of metastatic bone disease
- RNA modifications and cancer
- Cancer Research and Treatments
- Cancer-related molecular mechanisms research
- Cerebrospinal fluid and hydrocephalus
- Cancer Mechanisms and Therapy
- Cancer Cells and Metastasis
- Cell death mechanisms and regulation
- Natural Compounds in Disease Treatment
- Extracellular vesicles in disease
University Medical Center Hamburg-Eppendorf
2020-2024
Universität Hamburg
2020-2024
University of Chicago
2022-2024
Charité - Universitätsmedizin Berlin
2015-2020
Extracellular vesicles (EVs) are naturally occurring nano-sized carriers that secreted by cells and facilitate cell-to-cell communication their unique ability to transfer biologically active cargo. Despite the pronounced increase in our understanding of EVs over last decade, from disease pathophysiology therapeutic drug delivery, improved molecular tools track delivery still needed. Unfortunately, present catalogue utilised for EV labelling lacks sensitivity or not sufficiently specific....
The majority of cancer patients receive radiotherapy during the course treatment, delivered with curative intent for local tumor control or as part a multimodality regimen aimed at eliminating distant metastasis. A major focus research has been DNA damage; however, in past two decades, emphasis shifted to important role immune system plays radiotherapy-induced anti-tumor effects. Radiotherapy reprograms microenvironment, triggering and RNA sensing cascades that activate innate immunity...
Abstract Purpose: Radiotherapy (RT) is a widely employed anticancer treatment. Emerging evidence suggests that RT can elicit both tumor-inhibiting and tumor-promoting immune effects. The purpose of this study to investigate suppressive factors radiotherapy. Experimental Design: We used heterologous two-tumor model in which adaptive concomitant immunity was eliminated. Results: Through analysis PD-L1 expression myeloid-derived suppressor cells (MDSC) frequencies using patient peripheral blood...
Up to 40% of advance lung, melanoma and breast cancer patients suffer from brain metastases (BM) with increasing incidence. Here, we assessed whether circulating tumor cells (CTCs) in peripheral blood can serve as a disease surrogate, focusing on CD44 CD74 expression prognostic markers for BM. We show that size-based microfluidic approach combination semi-automated cell recognition system are well suited CTC detection BM allow further characterization potentially derived CTCs were found 50%...
Activation of TGF-β signaling serves as an extrinsic resistance mechanism that limits the potential for radiotherapy. Bone morphogenetic protein and activin membrane-bound inhibitor (BAMBI) antagonizes is implicated in cancer progression. However, molecular mechanisms BAMBI regulation immune cells its impact on antitumor immunity after radiation have not been established. Here, we show ionizing (IR) specifically reduces expression immunosuppressive myeloid-derived suppressor (MDSCs) both...
RNA N6-methyladenosine (m6A) reader YTHDF1 is implicated in cancer etiology and progression. We discovered that radiotherapy (RT) increased expression dendritic cells (DCs) of PBMCs from patients, but not other immune tested. Elevated DCs was associated with poor outcomes patients receiving RT. found loss Ythdf1 enhanced the antitumor effects ionizing radiation (IR) via increasing cross-priming capacity across multiple murine models. Mechanistically, IR upregulated through STING-IFN-I...
Abstract Prognosis of patients with high-grade aneurysmal subarachnoid hemorrhage (aSAH) is only insufficiently displayed by current standard prognostic scores. This study aims to evaluate the role pupil status for mortality prediction and provide improved models. Anonymized data 477 aSAH admitted our medical center from November 2010 August 2018 were retrospectively analyzed. Identification variables independently predicting in-hospital was performed multivariable logistic regression...
Radiation-mediated immune suppression limits efficacy and is a barrier in cancer therapy. Radiation induces negative regulators of tumor immunity including regulatory T cells (Treg). Mechanisms underlying Treg infiltration after radiotherapy (RT) are poorly defined. Given that conventional dendritic (cDC) maintain Treg, we sought to identify target cDC signaling block radiation.
The influence of preexisting factors on the clinical course patients with subarachnoid hemorrhage (SAH), such as patient age, arterial hypertension, and aneurysm characteristics, is still a matter debate. However, specific impact exact location has not received adequate attention. Therefore, aim this study was to investigate factor mortality.The data consecutive aneurysmal SAH who were treated from October 2010 July 2020 retrospectively analyzed. We distinguished four locations: anterior...
Exoscopic 3D4K video systems (EX) have recently been introduced as an alternative to conventional operating microscopes (OM). However, experience in handling and setup remains scarce. This study evaluated the practicability of exoscopic surgery a wide range procedures. All EX operations performed between August 2018 May 2020 were compared with procedures using OM. A standardized questionnaire was used evaluate surgeons' comfort, image quality (including fluorescence modes), handling. In all,...
Abstract Objectives Infection with severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) causes disease 2019 (COVID‐19). Although an SARS‐CoV‐2 infection mainly presents illness, neurologic symptoms and sequelae are increasingly recognised in the long‐term treatment of COVID‐19 patients. The pathophysiology neuropathogenesis behind complications remain poorly understood, but mounting evidence points to endothelial dysfunction either directly caused by viral or indirectly inflammatory...
During the metastatic process tumor cells circulate in blood stream and are carried to various organs. In order spread different organs cell—endothelial cell interactions crucial for extravasation mechanisms. It remains unclear if dissemination spinal bone occurs by passive entrapment of circulating or active cellular mechanisms mediated surface molecules secreted factors. We investigated seeding three lines (melanoma, lung prostate carcinoma) microvasculature Their was compared biologically...
Non-small cell lung cancer (NSCLC) is currently the leading cause of cancer-related death worldwide, and incidence brain metastases (BM) in NSCLC patients continuously increasing. The recent improvements systemic treatment necessitate continuous updates on prognostic subgroups factors determining overall survival (OS). In order to improve clinical decision-making tumor boards, we investigated determinants affecting with resectable BM. A retrospective analysis was conducted surgically BM...
Objective: Spinal metastatic disease remains a major problem of oncological diseases. Patients affected may suffer pain, spinal instability, and severe neurological deficits. Today, palliative surgery radiotherapy are the mainstays therapy. In contrast, preventive treatment strategies or concepts for an early stage lacking. Here, we have used syngeneic, experimental spine metastases model in mouse to test efficacy mTOR inhibition anti-angiogenesis on formation progression melanoma...
Background Aneurysmal subarachnoid hemorrhage (SAH) presents occasionally with cardiac arrest (CA). The impact of pre-hospital and emergency room (ER) treatment on outcome remains unclear. Therefore, we investigated the treatment, focusing lay cardiopulmonary resuscitation (CPR), ER handling SAH patients out-of-hospital CA (OHCA). Methods In this bi-centric retrospective analysis, reviewed databases for OHCA CPR from January 2011 to June 2021. Patients were analyzed general clinical...
Tumor–endothelial cell interactions represent an essential mechanism in spinal metastasis. Ephrin-B2–EphB4 communication induces tumor repulsion from the endothelium metastatic melanoma, reducing bone metastasis formation. To shed further light on signaling mechanism, we researched effects of pharmacological EphB4 receptor stimulation and inhibition a ligand-dependent/independent context. We chose preventative post-diagnostic therapeutic window. during seeding led to increase loci number...
Abstract Background Lung cancer is the leading cause of cancer-related deaths worldwide, with brain metastasis (BM) occurring in 40% advanced non-small cell lung (NSCLC) patients. In 15% these patients, only affected organ (oligo-metastasis), corresponding to improved prognosis compared widespread disease. Thus far, it unknown if metastatic dissemination without systemic metastases a consequence immune system’s ability control tumor outgrowth. Methods Here, we investigated local and...
<p>Supplementary figure 3. Gating strategy and PD-L1 in subsets of patient PBMCs</p>
<p>Supplementary figure 1. Radiation is not able to induce an abscopal effect. A. Growth of LLC flank local tumors received RT. B. Flow cytometry result whole lung gated on CD45- CD31- population. C. q-PCR indicating expression genes in sorted cells (Mets), with and MC38 cell lines as controls. The relative presented fold over those LLC. D. Quantification metastasis the MC38/LLC tumor model by percentage area. E. heterogeneous 2-tumor (MC38/B16F10) which ctrl (sham treatment) or IR. F....