A5077308963- Extracellular vesicles in disease
- RNA Interference and Gene Delivery
- MicroRNA in disease regulation
- Cell Adhesion Molecules Research
- Advanced biosensing and bioanalysis techniques
- Nanoplatforms for cancer theranostics
- Chronic Lymphocytic Leukemia Research
- CRISPR and Genetic Engineering
- Immunodeficiency and Autoimmune Disorders
- RNA modifications and cancer
- Cytokine Signaling Pathways and Interactions
- Circular RNAs in diseases
- Graphene and Nanomaterials Applications
- RNA regulation and disease
- Exercise and Physiological Responses
- Nanopore and Nanochannel Transport Studies
- Complement system in diseases
- Virus-based gene therapy research
- RNA Research and Splicing
- Dendrimers and Hyperbranched Polymers
- Anodic Oxide Films and Nanostructures
- Neurogenetic and Muscular Disorders Research
- Fibroblast Growth Factor Research
- Lipid metabolism and disorders
- Nerve injury and regeneration
Karolinska Institutet
2018-2025
University of Oxford
2023-2025
Karolinska University Hospital
2018-2024
Aarhus University
2024
University of Southern Denmark
2024
Aarhus University Hospital
2024
PsiOxus Therapeutics (United Kingdom)
2019-2023
John Radcliffe Hospital
2023
Extracellular vesicles (EVs) can be harvested from cell culture supernatants and all body fluids. EVs conceptually classified based on their size biogenesis as exosomes microvesicles. Nowadays, it is however commonly accepted in the field that there a much higher degree of heterogeneity within these two subgroups than previously thought. For instance, surface marker profile likely dependent source, cell's activation status, multiple other parameters. Within recent years, several new methods...
Extracellular vesicles (EVs) are naturally occurring nano-sized carriers that secreted by cells and facilitate cell-to-cell communication their unique ability to transfer biologically active cargo. Despite the pronounced increase in our understanding of EVs over last decade, from disease pathophysiology therapeutic drug delivery, improved molecular tools track delivery still needed. Unfortunately, present catalogue utilised for EV labelling lacks sensitivity or not sufficiently specific....
Abstract Extracellular vesicles (EVs) form an endogenous transport system for intercellular transfer of biological cargo, including RNA, that plays a pivotal role in physiological and pathological processes. Unfortunately, whereas effects EV-mediated RNA are abundantly studied, regulatory pathways mechanisms remain poorly defined due to lack suitable readout systems. Here, we describe highly-sensitive CRISPR-Cas9-based reporter allows direct functional study small non-coding molecules at...
Extracellular vesicles (EVs) function as natural delivery vectors and mediators of biological signals across tissues. Here, by leveraging these functionalities, we show that EVs decorated with an antibody-binding moiety specific for the fragment crystallizable (Fc) domain can be used a modular system targeted cancer therapy. The Fc-EVs different types immunoglobulin G antibody thus to virtually any tissue interest. Following optimization engineered screening Fc-binding EV-sorting moieties,...
We have determined whether orange juice-derived nanovesicles (ONVs) could be used for the treatment of obesity-associated intestinal complications. ONVs were characterized by lipidomic, metabolomic, electron microscopy. In vitro, barriers (IBs = Caco-2+HT-29-MTX) treated with and co-cultured adipocytes to monitor IB fat release. vivo, obesity was induced a high-fat, high-sucrose diet (HFHSD mice) 12 weeks. Then, half HFHSD mice gavaged ONVs. One-month ONV did not modify HFHSD-induced insulin...
Extracellular vesicles (EVs) have shown promise as potential therapeutics for the treatment of various diseases. However, their rapid clearance after administration could be a limitation in certain therapeutic settings. To solve this, an engineering strategy is employed to decorate albumin onto surface EVs through display binding domains (ABDs). ABDs were either included extracellular loops select EV-enriched tetraspanins (CD63, CD9 and CD81) or directly fused terminal single transmembrane...
Extracellular vesicles (EVs) have been established to play important roles in cell-cell communication and shown promise as therapeutic agents. However, we still lack a basic understanding of how cells respond upon exposure EVs from different cell sources at various doses. Thus, treated fibroblasts with 12 doses between 20 200,000 per cell, analyzed their transcriptional effects, functionally confirmed the findings types vitro, vivo using single-cell RNA sequencing. Unbiased global analysis...
Metal-organic frameworks (MOFs) have emerged as promising novel therapeutics for treating malignancies due to their tunable porosity, biocompatibility, and modularity functionalize with various chemotherapeutics drugs. However, the design synthesis of dual-stimuli responsive MOFs controlled drug release in tumor microenvironments are vitally essential but still challenging. Meanwhile, catalytic effect metal ions selection ratio optimization enhanced chemodynamic therapy (CDT) is relatively...
Extracellular vesicles (EVs) are gaining ground as next-generation drug delivery modalities. Genetic fusion of the protein interest to a scaffold with high EV-sorting ability represents robust cargo loading strategy. To address paucity such proteins, we leverage simple and reliable assay that can distinguish intravesicular proteins from surface- well non-vesicular compare potential 244 candidate proteins. We identify 24 conserved abilities across five types producer cells. TSPAN2 TSPAN3...
Abstract Some viruses are rarely transmitted orally or sexually despite their presence in saliva, breast milk, semen. We previously identified that extracellular vesicles (EVs) semen and saliva inhibit Zika virus infection. However, the antiviral spectrum underlying mechanism remained unclear. Here we applied lipidomics flow cytometry to show these EVs expose phosphatidylserine (PS). By blocking PS receptors, targeted by process of apoptotic mimicry, they interfere with viral attachment...
Small extracellular vesicles (sEVs) generated from the endolysosomal system, often referred to as exosomes, have attracted interest a suitable biomarker for cancer diagnostics, they carry valuable biological information and reflect their cells of origin. Herein, we propose simple inexpensive electrical method label-free detection profiling sEVs in size range exosomes. The is based on electrokinetic principle, where change streaming current monitored surface markers interact with affinity...
Extracellular vesicles (EVs) are nanosized cell-derived produced by all cells, which provide a route of intercellular communication transmitting biological cargo. While EVs offer promise as therapeutic agents, the molecular mechanisms EV biogenesis not yet fully elucidated, in part due to concurrence numerous interwoven pathways give rise heterogenous populations vitro. The equilibrium between EV-producing is heavily influenced factors extracellular environment, such way that can be taken...
Extracellular vesicles (EVs) are efficient natural vehicles for intercellular communication and under extensive investigation the delivery of diverse therapeutics including small molecule drugs, nucleic acids, proteins. To understand mechanisms behind biological activities EVs develop EV therapeutics, it's fundamental to track engineer in a customized manner. In this study, we identified, using single-vesicle flow cytometry microscopy, lipid DOPE (dioleoyl phosphatidyl ethanolamine) as an...
Abstract Messenger RNA (mRNA) has emerged as an attractive therapeutic molecule for a plethora of clinical applications. For in vivo functionality, mRNA therapeutics require encapsulation into effective, stable, and safe delivery systems to protect the cargo from degradation reduce immunogenicity. Here, bioengineering platform efficient loading functional using bionormal nanoparticles, extracellular vesicles (EVs), is established by expressing highly specific RNA‐binding domain fused CD63 EV...
Extracellular vesicles (EVs) are promising tools to transfer macromolecular therapeutic molecules recipient cells, however, efficient functional intracellular protein delivery by EVs remains challenging. Here, we have developed novel and versatile systems that leverage selected molecular engineer for robust cytosolic both in vitro vivo. These systems, termed VSV-G plus EV-sorting Domain-Intein-Cargo (VEDIC) VSV-G-Foldon-Intein-Cargo (VFIC), exploit an engineered mini-intein (intein) with...
Abstract Extracellular vesicles (EVs) are lipid nanoparticles and play an important role in cell‐cell communications, making them potential therapeutic agents allowing to engineer for targeted drug delivery. The expanding applications of EVs next generation medicine is still limited by existing tools scaling standardized EV production, single tracing analytics, thus provide only a snapshot tissue‐specific cargo information. Here, we present the Snorkel‐tag, which have genetically fused...
The toolbox for genetic engineering has quickly evolved from CRISPR/Cas9 to a myriad of different gene editors, each with promising properties and enormous clinical potential. However, major challenge remains: delivering the CRISPR machinery nucleus recipient cells in nontoxic efficient manner. In this article, we repurpose an RNA-delivering cell-penetrating peptide, PepFect14 (PF14), deliver Cas9 ribonucleoprotein (RNP). RNP-CPP complex achieved high editing rates, e.g., up 80% HEK293T...