A5068740520- Extracellular vesicles in disease
- RNA Interference and Gene Delivery
- MicroRNA in disease regulation
- Cell Adhesion Molecules Research
- Environmental Education and Sustainability
- Autophagy in Disease and Therapy
- Calcium signaling and nucleotide metabolism
- Environmental Toxicology and Ecotoxicology
- Pluripotent Stem Cells Research
- Mosquito-borne diseases and control
- CAR-T cell therapy research
- Carcinogens and Genotoxicity Assessment
- Monoclonal and Polyclonal Antibodies Research
- Tissue Engineering and Regenerative Medicine
- Climate Change Communication and Perception
- Mitochondrial Function and Pathology
- RNA Research and Splicing
- Neuroinflammation and Neurodegeneration Mechanisms
- Social Acceptance of Renewable Energy
- Sphingolipid Metabolism and Signaling
- Alzheimer's disease research and treatments
- Erythrocyte Function and Pathophysiology
- Advanced biosensing and bioanalysis techniques
- Amyotrophic Lateral Sclerosis Research
- Cancer Research and Treatments
University of Salzburg
2014-2025
Extracellular vesicles (EVs) are nanosized intercellular messengers that bear enormous application potential as biological drug delivery vehicles. Much progress has been made for loading or decorating EVs with proteins, peptides RNAs using genetically engineered donor cells, but post-isolation synthetic drugs and from natural sources remains challenging. In particular, quantitative unambiguous data assessing whether how small molecules associate versus other components in the samples still...
Extracellular vesicles (EVs) are efficient natural vehicles for intercellular communication and under extensive investigation the delivery of diverse therapeutics including small molecule drugs, nucleic acids, proteins. To understand mechanisms behind biological activities EVs develop EV therapeutics, it's fundamental to track engineer in a customized manner. In this study, we identified, using single-vesicle flow cytometry microscopy, lipid DOPE (dioleoyl phosphatidyl ethanolamine) as an...
Abstract Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) constitute a promising therapy for myocardial infarction (MI). The lack of an effective immunosuppressive regimen, combined with single-cell transplantations, results in suboptimal outcomes, such as poor engraftment and compromised therapeutic efficacy. This study aimed to confirm the increased retention hiPSC-CMs microtissues (CMTs) over grafts. To ensure long-term survival CMTs potential cardiac applications,...
Extracellular vesicle (EV) research increasingly demands for quantitative characterisation at the single level to address heterogeneity and complexity of EV subpopulations. Emerging, commercialised technologies analysis based on, example, imaging flow cytometry or after capture on chips generally require dedicated instrumentation proprietary software not readily accessible every lab. This limits their implementation routine in rapidly growing field. We others have shown that vesicles can be...
Abstract Extracellular vesicles (EVs) are highly interesting for the design of next-generation therapeutics. However, their preparation methods face challenges in standardization, yield, and reproducibility. Here, we describe a efficient reproducible EV method monodisperse nano plasma membrane (nPMVs), which yields 10 to 100 times more particles per cell hour than conventional methods. nPMVs produced by homogenizing giant following blebbing apoptotic body secretion induced chemical...
An intronic G4C2 repeat expansion in the C9ORF72 gene is major known cause for Amyotrophic Lateral Sclerosis (ALS), with current evidence both, loss of function and pathological gain disease mechanisms. We screened 96 200 small molecules patient iPS neurons modulation nuclear RNA foci identified 82 validated hits, including Brd4 inhibitor JQ1 as well novel analogs Spliceostatin-A, a modulator SF3B1, branch point binding protein U2-snRNP. Spliceosome by these SF3B1 targeted compounds recruits...
BACKGROUND/AIMS: Trypan blue is routinely used in cell culture experiments to distinguish between dead cells, which are labelled by trypan blue, and viable apparently free of any staining. The assumption that labelling restricted cells derives from the observation rupture plasma membrane correlates with intense However, decades ago, has been trace fluid uptake macrophage-like animals. These studies contributed concept reticuloendothelial system vertebrates. itself does not show a...
Lipid-containing vacuoles in microglia were discovered more than one hundred years ago the brain of patients showing neurodegenerative processes. Recently, molecular-biological studies demonstrated specific changes lipid-metabolism related to neurodegeneration. Despite that already Alzheimer described a distinct glia phenotype having large, lipid-containing (Gitterzellen), little is known about how convert lipid metabolites into vacuolated phenotype. We studied impact liver-derived,...