A5085810556- Traumatic Brain Injury and Neurovascular Disturbances
- Cerebrospinal fluid and hydrocephalus
- Forensic Fingerprint Detection Methods
- Forensic and Genetic Research
- Angiogenesis and VEGF in Cancer
- Blood properties and coagulation
- Advanced Neuroimaging Techniques and Applications
- Nitric Oxide and Endothelin Effects
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Coronary Interventions and Diagnostics
- Intracranial Aneurysms: Treatment and Complications
- Dermatologic Treatments and Research
- Atherosclerosis and Cardiovascular Diseases
- Apelin-related biomedical research
- Cardiovascular Health and Disease Prevention
- Platelet Disorders and Treatments
- Renin-Angiotensin System Studies
- Cardiac pacing and defibrillation studies
- Cardiac Arrhythmias and Treatments
- Cardiac, Anesthesia and Surgical Outcomes
- Cardiac Valve Diseases and Treatments
- Cardiomyopathy and Myosin Studies
- Barrier Structure and Function Studies
- DNA Repair Mechanisms
- Tissue Engineering and Regenerative Medicine
Amsterdam University Medical Centers
2019-2025
University of Amsterdam
2013-2025
Maastricht University
2024
Maastricht University Medical Centre
2024
Amsterdam Neuroscience
2020-2023
Amsterdam UMC Location University of Amsterdam
2008-2018
Centre for Biomedical Engineering and Physics
2009-2010
Onze Lieve Vrouwziekenhuis Hospital
1999
Sint Lucas Andreas Hospital
1996
Clearance of waste products from the brain is vital importance. Recent publications suggest a potential clearance mechanism via paravascular channels around blood vessels. Arterial pulsations might provide driving force for flow, but its flow pattern remains poorly characterized. In addition, relationship between leptomeningeal vessels and penetrating unclear. this study, we determined diameter through thinned-skull cranial window. We observed that microspheres moved preferentially in space...
Recent evidence suggests an extensive exchange of fluid and solutes between the subarachnoid space brain interstitium, involving preferential pathways along blood vessels. We studied anatomical relations vasculature, cerebrospinal compartments, paravascular spaces in male Wistar rats. A fluorescent tracer was infused into cisterna magna, without affecting intracranial pressure. Tracer distribution analyzed using a 3D imaging cryomicrotome, confocal microscopy, correlative light electron...
In the absence of a true lymphatic system in brain parenchyma, alternative clearance pathways for excess fluid and waste products have been proposed. Suggested mechanisms implicate role interstitial cerebrospinal fluids. However, proposed direction flow, anatomical structures involved, driving forces are controversial. To trace distribution brain, to identify we infused mix fluorescent tracers with different sizes into cisterna magna or striatum mouse brains. We subsequently performed...
Abstract Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) constitute a promising therapy for myocardial infarction (MI). The lack of an effective immunosuppressive regimen, combined with single-cell transplantations, results in suboptimal outcomes, such as poor engraftment and compromised therapeutic efficacy. This study aimed to confirm the increased retention hiPSC-CMs microtissues (CMTs) over grafts. To ensure long-term survival CMTs potential cardiac applications,...
Abstract No forensic method exists that can reliably estimate the age of fingermarks found at a crime scene. Information on time passed since fingermark deposition is desired as it be used to distinguish between related and unrelated support or refute statements made by donors. We introduce non‐contact fingermarks. Fingermarks were approached protein–lipid mixtures an age‐estimation model was build based expected protein lipid oxidation reactions. Two measures are required from its age: 1)...
Objectives: To review the presenting clinical signs, treatment and survival of dogs with tonsillar squamous cell carcinoma and, if possible, to identify useful prognostic indicators. Methods: Medical records 44 were reviewed retrospectively. Clinical stage, time diagnosis, outcome recorded. Data analysed using Kaplan-Meier, log-rank, Student's t test, Kruskal-Wallis test Chi-square/Fisher Exact as appropriate. Results: The most frequent signs cough (12 dogs, 27%), enlarged lymph nodes (11...
Thrombospondin 4 (TSP-4) expression is induced in the heart and vasculature under pathological conditions, including myocardial infarction, pressure overload, hypertension. TSP-4 linked to remodelling processes, where it may affect extracellular matrix protein organization. In previous work, we studied role of small arteries during hypertension using Ang II-treated knockout (Thbs4−/−) mice. We reported increased weight, as well occurrence aortic aneurysms Thbs4−/− animals. present study,...
Hypertension is associated with chronic vascular inflammation. We tested the hypothesis that sensitivity to develop hypertension and remodeling depends on immunological background. Blood pressure, remodeling, endothelial function, architecture (number of collateral arteries), expression inflammatory cytokines were determined in mice received N(G)-nitro-l-arginine methyl ester (l-NAME) inhibit nitric oxide synthesis. studied C57BL/6, BALB/c, BALB.B6-Cmv1r mice, a congenic strain where natural...
Abstract Hypertension is associated with cognitive decline and various forms of dementia, including Alzheimer’s disease. In animal models hypertension, many disease characteristics are recapitulated, brain atrophy, decline, amyloid β accumulation blood barrier dysfunction. Removal other waste products depends in part on clearance via the interstitial fluid (ISF). Here we studied impact hypertension ISF drainage, using spontaneously hypertensive rats (SHR) normotensive Wistar Kyoto (WKY). At...
Hypertension is an important risk factor for cerebrovascular disease, including stroke and dementia. Both in humans animal models of hypertension, neuropathological features such as brain atrophy oedema have been reported. We hypothesised that damage resulting from chronic hypertension would manifest itself a more permeable blood-brain barrier blood-cerebrospinal fluid barrier. In addition, leaky barriers could potentially contribute to enhanced interstitial cerebrospinal formation, which...
Abstract Background Proper neuronal function is directly dependent on the composition, turnover, and amount of interstitial fluid that bathes cells. Most likely to be derived from ion water transport across brain capillary endothelium, a process may altered in hypertension due vascular pathologies as endothelial dysfunction arterial remodelling. In current study, we investigated effects for differences homeostasis. Methods Magnetic resonance imaging (MRI) was performed 7T small animal MRI...
Midlife hypertension is an important risk factor for cognitive impairment and dementia, including Alzheimer's disease. We investigated the effects of long-term treatment with two classes antihypertensive drugs to determine whether diverging mechanisms blood pressure lowering impact brain differently. Spontaneously hypertensive rats (SHR) were either left untreated or treated a calcium channel blocker (amlodipine) beta (atenolol) until one year age. The normotensive Wistar Kyoto rat (WKY) was...
A total of 1124 consecutive patients who were selected for coronary artery bypass graft surgery studied. Of awaiting (mean waiting time 98 days) 25 (2.2%) died before operation 63 days). To assess patient characteristics predictive early mortality surgery, deceased analysed and compared to 50 controls matched age, gender, type waiting-list priority. Univariate analysis showed that the had a higher rate severe angina pectoris class III-IV (odds ratio (OR) 2.9), unstable prior angiography (OR...
In human medicine, primary frontal sinus squamous cell carcinoma (pFS‐SCC) is not frequently reported. veterinary SCC exclusively described as an extension of nasal cavity SCC. To our knowledge, this the first publication concerning canine pFS‐SCC, diagnosed using histology or cytology and medical imaging, in three dogs. The tumours extended into orbit brain cavity, without involvement. Treatment was initiated with piroxicam–carboplatin. Prolongation carboplatin delivery a low dose intensity...
Cardiac remodelling and heart failure are promoted by persistent sympathetic activity. We recently reported that nuclear receptor Nur77 may protect against agonist-induced cardiac in mice. The co-transmitter neuropeptide Y (NPY) is co-released with catecholamines a known modulator predictor of mortality. Recently, transcriptome analyses revealed NPY as putative target Nur77. In this study, we assess whether modulates adverse via signalling. represses expression the PC12 adrenal chromaffin...
Small arteries are known to develop functional and structural alterations in hypertension. However, the mechanisms of this remodeling not fully understood. We hypothesized that altered gene expression is associated with development hypertension mesenteric spontaneously hypertensive rats (SHR). Three sublines SHR normotensive Wistar Kyoto (WKY) were studied at 6 weeks 5 months age. MiRNA mRNA microarray experiments performed analyzed bioinformatical tools, including Ingenuity Pathway Analysis...
Background and Purpose- We developed a rat model of silent brain infarcts based on microsphere infusion investigated their impact perfusion tissue damage. Second, we studied the extent mechanisms recovery. Methods- At day 0, 15 µm fluorescent microspheres were injected into right common carotid artery F344 rats. days 1, 7, or 28, was removed, cut in 100-µm cryosections, processed for immunofluorescent staining analysis. Results- Injection caused mild transient damage to treated hemisphere,...
Objectives Spontaneously hypertensive rats (SHR) have been used frequently as a model for human essential hypertension. However, both the SHR and its normotensive control, Wistar Kyoto rat (WKY), consist of genetically different sublines. We tested hypothesis that pathophysiology vascular remodeling in hypertension differs among Methods Results studied mesenteric resistance arteries WKY from three sources, at 6 weeks 5 months age. Sublines showed differences blood pressure, body weight,...
Placental insufficiency may lead to preeclampsia and fetal growth restriction. There is no cure for placental insufficiency, emphasizing the need monitoring placenta health. Current methods are limited, underscoring necessity imaging techniques evaluate fetal-placental perfusion oxygenation. This study aims use MRI oxygenation in reduced uterine pressure (RUPP) model of insufficiency.
Glycosaminoglycans in the skin interstitium and endothelial surface layer have been shown to be involved local sodium accumulation without commensurate water retention. Dysfunction of heparan sulfate glycosaminoglycans may therefore disrupt homeostasis. In this study, we investigated effects combined heterozygous loss polymerization genes (exostosin glycosyltransferase 1 2; Ext1+/-Ext2+/-) on Sodium storage capacity was decreased Ext1+/-Ext2+/- mice as reflected by a 77% reduction thickness...
Pulsed dye lasers are used effectively in the treatment of psoriasis with long remission time and limited side effects. It is, however, not completely understood which biological processes underlie its favorable outcome. laser at 585-595 nm targets hemoglobin blood, inducing local hyperthermia surrounding blood vessels adjacent tissues. While impact destructive temperatures on has been well studied, effects lower function several cell types within vessel wall periphery known. The aim our...