A5026622926- Photoacoustic and Ultrasonic Imaging
- Optical Imaging and Spectroscopy Techniques
- Optical Coherence Tomography Applications
- Photodynamic Therapy Research Studies
- Forensic and Genetic Research
- Forensic Fingerprint Detection Methods
- Nanoplatforms for cancer theranostics
- Spectroscopy Techniques in Biomedical and Chemical Research
- Forensic Entomology and Diptera Studies
- Esophageal Cancer Research and Treatment
- Forensic Anthropology and Bioarchaeology Studies
- Advanced Fluorescence Microscopy Techniques
- Cultural Heritage Materials Analysis
- Porphyrin and Phthalocyanine Chemistry
- Paleopathology and ancient diseases
- Gastric Cancer Management and Outcomes
- Infrared Thermography in Medicine
- Glaucoma and retinal disorders
- Conservation Techniques and Studies
- Spectroscopy and Chemometric Analyses
- Non-Invasive Vital Sign Monitoring
- Molecular Biology Techniques and Applications
- Luminescence Properties of Advanced Materials
- Thermoregulation and physiological responses
- bioluminescence and chemiluminescence research
University of Amsterdam
2016-2025
Amsterdam University Medical Centers
2018-2025
Public Health Service of Amsterdam
2024-2025
Amsterdam UMC Location University of Amsterdam
2011-2021
Netherlands Institute for the Study of Crime and Law Enforcement
2016
University of Minnesota
2014
GGD Amsterdam
2014
Centre for Biomedical Engineering and Physics
2002-2013
The Netherlands Cancer Institute
2001-2007
Hy-Line (United States)
2005
A highly efficient multifunctional nanoplatform for simultaneous upconversion luminescence (UCL) imaging and photodynamic therapy has been developed on the basis of selective energy transfer from multicolor luminescent NaYF(4):Yb(3+),Er(3+) nanoparticles (UCNPs) to photosensitizers (PS). Different popular approaches based electrostatic or hydrophobic interactions, over 100 photosensitizing molecules were covalently bonded every 20 nm UCNP, which significantly strengthened UCNP-PS linkage...
A method is described for measuring optical properties and deriving chromophore concentrations from diffuse reflection measurements at the surface of a turbid medium. The uses diffusion approximation model reflectance, in combination with models absorption scattering coefficients. An fibre-based set-up, capable nine spectra 400 to 1050 nm simultaneously, used test experimentally. Results analyses phantom vivo are presented. These demonstrate that wavelength range 600 900 nm, tissue can be as...
From calibrated, weakly scattering tissue phantoms (2-6 mm -1 ), we extract the attenuation coefficient with an accuracy of 0.8 from OCT data in clinically relevant 'fixed focus' geometry.The are analyzed using a single model and recently developed description confocal point spread function (PSF).We verify validity by quantitative comparison multiple model, validate use PSF on calibrated samples.Implementation this for existing systems will be straightforward.Localized measurement different...
We report on the scattering properties of oxygenated and deoxygenated whole blood from 250 to 1000 nm. determine complex refractive index hemoglobin using a Kramers-Kronig analysis optical coherence tomography measurements. Combining these data with Mie theory, are calculated. The strong oxygen saturation dependent effects should be taken into account in oxymetry.
Almost all the luminescence upconversion nanoparticles used for Förster resonant energy transfer (FRET) applications are bare cores based on consideration that efficiency is optimized because distance between donors and acceptors minimized. On other hand, it well proved core/shell structure efficient in minimizing nonradiative loss of excitation donors. In this work we use NaYF4:Yb3+,Er3+@NaYF4 rose bengal photosensitizer to construct a FRET conjugate. From photophysics singlet oxygen...
Abstract Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) constitute a promising therapy for myocardial infarction (MI). The lack of an effective immunosuppressive regimen, combined with single-cell transplantations, results in suboptimal outcomes, such as poor engraftment and compromised therapeutic efficacy. This study aimed to confirm the increased retention hiPSC-CMs microtissues (CMTs) over grafts. To ensure long-term survival CMTs potential cardiac applications,...
The growing resistance against antifungal agents has renewed the search for alternative treatment modalities, and antimicrobial photodynamic inactivation (PDI) is a potential candidate. cationic porphyrin 5-phenyl-10,15,20-Tris(N-methyl-4-pyridyl)porphyrin chloride (TriP[4]) photosensitizer that in combination with light can inactivate bacteria, fungi, viruses. For future improvement of efficacy PDI clinically relevant fungi such as Candida albicans, we sought to understand working mechanism...
The optical properties (absorption and scattering coefficients the anisotropy factor) were measured in vitro for cartilage, liver, lung, muscle, myocardium, skin, tumour (colon adenocarcinoma CC 531) at 630, 632.8, 790, 850 1064 nm. Rabbits, rats, piglets, goats, dogs used to obtain tissues. A double-integrating-sphere setup with an intervening sample was determine reflectance, diffuse collimated transmittances of sample. inverse adding - doubling algorithm from measurements. overall results...
The combination of optical coherence tomography and spectroscopy may allow for highly localized, quantitative measurements tissue spectral properties. We present, the first time to our knowledge, absorption coefficients phantoms hemoglobin oxygenated with spectroscopic (SOCT). Our results suggest that SOCT will be able provide oxygenation measurements.
The authors studied the axial point spread function of optical coherence tomography for Gaussian intensity profiles emitted from and coupled back into single-mode fibers signals a scattering medium. determined Rayleigh length was roughly twice one measured reflection mirror. Using in combination with single backscatter model allowed determination attenuation coefficient suspension.
Optical coherence tomography (OCT) is a novel, high-resolution diagnostic tool that capable of imaging the arterial wall and plaques. The differentiation between different types atherosclerotic plaque based on qualitative differences in gray levels structural appearance. We hypothesize quantitative data analysis OCT signal allows measurement light attenuation by local tissue components, which can facilitate spatial discrimination constituents. High-resolution images (at 800 nm) human...
The use of spectroscopic optical coherence tomography to assess hemoglobin oxygen saturation whole blood is investigated. We propose the differential attenuation coefficient determine degree saturation. Our data show qualitative agreement between measured coefficients as a function and predictions based on oxygen-saturation-dependent absorption scattering properties blood.
Although transcutaneous bilirubinometers have existed for over 30 years, the clinical utility of technique is limited to a screening method hyperbilirubinemia, rather than replacement invasive blood sampling. In this study, we investigate reason value and address possibilities improvement.To obtain better insight into physiology bilirubin measurements, evaluated bilirubinometer that determines not only cutaneous concentration (TcB) but also volume fraction (BVF) in investigated skin volume....
Dried blood spot (DBS) sampling is recognized as a valuable alternative strategy both in research and clinical routine. Although many advantages are associated with DBS sampling, its more widespread use hampered by several issues, of which the hematocrit effect on DBS-based quantitation remains undoubtedly most widely discussed one. Previously, we developed method to derive approximate from nonvolumetrically applied based potassium content. this yielded good results was straightforward...
Optical coherence tomography (OCT) was used to determine optical properties of pelleted human fibroblasts in which necrosis or apoptosis had been induced. We analysed the OCT data, including both scattering medium and axial point spread function system. The attenuation coefficient necrotic cells decreased from 2.2 +/- 0.3 mm(1) 1.3 0.6 mm(-1), whereas, apoptotic cells, an increase 6.4 1.7 mm(-1) observed. results cultured as presented this study, indicate ability detect differentiate between...
Background In forensic science, age determination of bloodstains can be crucial in reconstructing crimes. Upon exiting the body, transit from bright red to dark brown, which is attributed oxidation oxy-hemoglobin (HbO2) met-hemoglobin (met-Hb) and hemichrome (HC). The fractions HbO2, met-Hb HC a bloodstain used for bloodstains. this study, we further analyze conversion HbO2 HC, determine effect temperature humidity on rates. Methodology bloodstain, as determined by quantitative analysis...
Abstract No forensic method exists that can reliably estimate the age of fingermarks found at a crime scene. Information on time passed since fingermark deposition is desired as it be used to distinguish between related and unrelated support or refute statements made by donors. We introduce non‐contact fingermarks. Fingermarks were approached protein–lipid mixtures an age‐estimation model was build based expected protein lipid oxidation reactions. Two measures are required from its age: 1)...