A5081891748- Electrospun Nanofibers in Biomedical Applications
- Tissue Engineering and Regenerative Medicine
- Bone Tissue Engineering Materials
- 3D Printing in Biomedical Research
- Polymer Surface Interaction Studies
- biodegradable polymer synthesis and properties
- Angiogenesis and VEGF in Cancer
- Blood properties and coagulation
- Cell Adhesion Molecules Research
- Additive Manufacturing and 3D Printing Technologies
- Mesenchymal stem cell research
- Graphene and Nanomaterials Applications
- Protease and Inhibitor Mechanisms
- Platelet Disorders and Treatments
- Cardiac and Coronary Surgery Techniques
- Innovative Microfluidic and Catalytic Techniques Innovation
- Nerve injury and regeneration
- Infectious Aortic and Vascular Conditions
- Hydrogels: synthesis, properties, applications
- Aortic aneurysm repair treatments
- Periodontal Regeneration and Treatments
- Proteoglycans and glycosaminoglycans research
- Silk-based biomaterials and applications
- Microfluidic and Bio-sensing Technologies
- Nanoparticle-Based Drug Delivery
University of Twente
2014-2024
Medisch Spectrum Twente
2021
AO Foundation
2013-2016
University of Amsterdam
2012
Amsterdam UMC Location University of Amsterdam
2012
Inserm
1988
The degradation and the tissue response evoked by poly(1,3-trimethylene carbonate) [poly(TMC)] copolymers of TMC with either 52 mol % D,L-lactide (DLLA) or 89 epsilon-caprolactone (CL) were evaluated in vivo subcutaneous implantation polymer films rats for periods up to one year. Poly(TMC) specimens extensively degraded after 3 weeks and, as confirmed histology, totally resorbed less than a A fast linear decrease thickness mass without change molecular weight was observed. Initially an acute...
Reproduction of the anatomical structures and functions tissues using cells designed 3D scaffolds is an ongoing challenge. For this, with appropriate biomorphic surfaces promoting cell attachment, proliferation differentiation are needed. In this study, eight triply-periodic minimal surface (TPMS)-based were specific trigonometric equations, providing same porosity number unit cells, while presenting different curvatures. The fabricated by stereolithography a photocurable resin based on...
Endothelial migration is an important process in the formation of blood vessels and repair damaged tissue. To study this laboratory, versatile reliable assays are essential. The purpose was to investigate whether microfluidic version conventional wound-healing assay a useful research tool for vascular science. cells were seeded 500-mum-wide channel. After overnight incubation, had formed viable confluent monolayer. Then, wound generated monolayer by flushing channel with three parallel fluid...
A fast increasing demand of medical products based on biomaterials and tissue engineering has led to an extensive growth in biomedical research the past two decades. highly interesting class are polymer-based composites, which nowadays widely used applications due their outstanding physical mechanical properties. In this paper, we aim summarize advancement composites with regard properties, structure fabrication using different techniques. Bioactive such as bone-forming, electrically...
Abstract The introduction of two-photon polymerization (2PP) to the field tissue engineering and regenerative medicine (TERM) has led great expectations for production scaffolds with an unprecedented degree complexity tailorable architecture. Unfortunately, resolution size are usually mutually exclusive when using 2PP, resulting in a lack highly-detailed relevant clinical application. Through combination highly reactive photopolymer optimizing key printing parameters, we propose first time...
A comparatively straightforward approach to accomplish more physiological realism in organ-on-a-chip (OoC) models is through substrate geometry. There increasing evidence that the strongly, microscale curved surfaces epithelial or endothelial cells experience when lining small body lumens, such as alveoli blood vessels, impact their behavior. However, most commonly used cell culture substrates for modeling of these human tissue barriers OoCs, ion track-etched porous membranes, provide only...
Copolymers of trimethylene carbonate and ε-caprolactone were synthesized characterized with the aim assessing their potential in development a flexible slowly degrading artificial nerve guide for bridging large defects. The effect monomer ratio on physical properties polymers its influence processability materials was investigated. Under applied polymerization conditions (130°C, 3 days using stannous octoate as catalyst) high molecular weight (Mn above 93 000) obtained. All copolymers had...
Biodegradable porous scaffolds for heart tissue engineering were prepared from amorphous elastomeric (co)polymers of 1,3-trimethylene carbonate (TMC) and D,L-lactide (DLLA). Leaching salt compression-molded polymer-salt composites allowed the preparation highly structures in a reproducible fashion. By adjusting particle size polymer-to-particle weight ratio composite pore porosity could be precisely controlled. The thermal properties polymers used scaffold had strong effect on morphology,...
The physiology of vascular endothelial cells is strongly affected by fluid shear stress on their surface. In this study, a microfluidic assay was employed to analyze the alignment actin filaments in response stress. When were cultured channels and subjected stress, channel direction significantly higher than static cultures. By adding inhibitory drugs, roles several signaling proteins process determined. Thus, it shown how technology can be provide mechanistic insight into cell physiology.
The aim of this study is to investigate the applicability flexible and elastic poly(trimethylene carbonate) (PTMC) structures prepared by stereolithography as scaffolds for cartilage tissue engineering. A three‐armed methacrylated PTMC macromer with a molecular weight 3100 g mol −1 used build designed pore diameter 350 ± 12 μm porosity 54.0 2.2%. Upon seeding bovine chondrocytes in scaffolds, cells adhere spread on surface. After culturing 6 weeks, also round morphology are present,...
Abstract Trimethylene carbonate (TMC) was copolymerized with D , L ‐lactide (DLLA) or ε ‐caprolactone (CL), and the degradation of melt‐pressed solid copolymer films in phosphate‐buffered saline at pH 7.4 37 °C followed for a period over two years. The parent homopolymers were used as reference materials. profile TMC‐DLLA‐ TMC‐CL based copolymers similar best described by autocatalyzed bulk hydrolysis, preferentially ester bonds. hydrolysis rates varied orders magnitude, depending on polymer...
Tissue engineering offers the opportunity to develop vascular scaffolds that mimic morphology of natural arteries. We have developed a porous three‐dimensional scaffold consisting fibres collagen and elastin interspersed together. Scaffolds were obtained by freeze‐drying suspension insoluble type I elastin. In order improve stability matrices, they cross‐linked two different methods. A water‐soluble carbodi‐imide, alone or in combination with diamine, was used for this purpose: zero‐...