A5000026424- Lipid Membrane Structure and Behavior
- Sexual Differentiation and Disorders
- Metabolism and Genetic Disorders
- Gut microbiota and health
- Hormonal and reproductive studies
- RNA Interference and Gene Delivery
- Hormonal Regulation and Hypertension
- Diet and metabolism studies
- Genetic Neurodegenerative Diseases
- Ovarian function and disorders
- Tryptophan and brain disorders
- Cholesterol and Lipid Metabolism
- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Adipose Tissue and Metabolism
- Peroxisome Proliferator-Activated Receptors
- Metabolomics and Mass Spectrometry Studies
- Lysosomal Storage Disorders Research
- RNA regulation and disease
- Advanced biosensing and bioanalysis techniques
- Fatty Acid Research and Health
- Liver Disease Diagnosis and Treatment
- Autophagy in Disease and Therapy
- Mitochondrial Function and Pathology
- Clostridium difficile and Clostridium perfringens research
Inserm
2015-2024
Sorbonne Université
2015-2024
Hôpital Saint-Antoine
2015-2024
Centre de Recherche Saint-Antoine
2009-2024
Fédération Hospitalo-Universitaire, Paris Center for Microbiome Medicine
2021-2024
Centre National de la Recherche Scientifique
2010-2024
Assistance Publique – Hôpitaux de Paris
2015-2024
Allen Institute for Brain Science
2024
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2024
Laboratoire des Biomolécules
2010-2022
Exosomes released from cells can transfer both functional proteins and RNAs between cells. In this study we tested the hypothesis that muscle might transmit specific signals during lipid-induced insulin resistance through exosomal route.Exosomes were collected quadriceps muscles of C57Bl/6 mice fed for 16 weeks with either a standard chow diet (SD) or an SD enriched 20% palm oil (HP) C2C12 exposed to 0.5 mmol/l palmitate (EXO-Post Palm), oleate Oleate) BSA BSA).HP-fed obese resistant had...
Huntington’s disease is an autosomal dominant neurodegenerative caused by abnormal polyglutamine expansion in huntingtin (Exp-HTT) leading to degeneration of striatal neurons. Altered brain cholesterol homeostasis has been implicated disease, with increased accumulation neurons yet reduced levels metabolic precursors. To elucidate these two seemingly opposing dysregulations, we investigated the expression 24-hydroxylase (CYP46A1), neuronal-specific and rate-limiting enzyme for conversion...
Dendrogenin A (DDA) is a newly discovered cholesterol metabolite with tumor suppressor properties. Here, we explored its efficacy and mechanism of cell death in melanoma acute myeloid leukemia (AML). We found that DDA induced lethal autophagy vitro vivo, including primary AML patient samples, independently Braf status or molecular cytogenetic classifications. partial agonist on liver-X-receptor (LXR) increasing Nur77, Nor1, LC3 expression leading to autolysosome formation. Moreover,...
Abstract Dysfunctions in brain cholesterol homeostasis have been extensively related to disorders. The main pathway for elimination is its hydroxylation into 24S-hydroxycholesterol by the 24-hydrolase, CYP46A1. Increasing evidence suggests that CYP46A1 has a role pathogenesis and progression of neurodegenerative disorders, increasing levels neuroprotective. However, mechanisms underlying this neuroprotection remain be fully understood. Huntington’s disease fatal autosomal dominant caused an...
The current pandemic of coronavirus disease (COVID) 2019 constitutes a global public health issue. Regarding the emerging importance gut-lung axis in viral respiratory infections, analysis gut microbiota's composition and functional activity during severe acute syndrome 2 (SARS-CoV-2) infection might be instrumental understanding controling COVID 19. We used nonhuman primate model (the macaque), that recapitulates mild COVID-19 symptoms, to analyze effects SARS-CoV-2 on dynamic changes...
Due to the potential role of gut microbiota and bile acids in pathogenesis both inflammatory bowel disease (IBD) sporadic colorectal cancer, we aimed determine whether these factors were associated with cancer IBD patients. 215 patients 51 non-IBD control subjects enrolled from 10 French centers between September 2011 July 2018. Fecal samples processed for bacterial 16S rRNA gene sequencing acid profiling. Demographic, clinical, endoscopic, histological outcomes recorded. Characteristics...
Schizophrenia is a severe mental condition in which several lipid abnormalities-either structural or metabolic-have been described. We tested the hypothesis that an abnormality membrane composition may contribute to aberrant dopamine signaling, and thereby symptoms cognitive impairment, schizophrenia (SCZ) patients. Antipsychotic-medicated clinically stable SCZ outpatients (n=74) were compared with matched healthy subjects (HC, n=40). A lipidomic analysis was performed red blood cell (RBC)...
Basic cell-penetrating peptides are potential vectors for therapeutic molecules and display antimicrobial activity. The peptide-membrane contact is the first step of sequential processes leading to peptide internalization cell However, molecular mechanisms involved in interaction not well understood frequently controversial. Herein, we compared membrane activities six basic with different size, charge density amphipaticity: Two (penetratin R9), three amphipathic neuromodulator substance...
Congenital adrenal hyperplasia (CAH) due to steroid 21-hydroxylase deficiency (CAH21) is most often diagnosed by newborn screening. The classic parameter studied 17-hydroxy-progesterone, but the positive predictive value for diagnosis of CAH low in full-term newborns and even lower preterm newborns.To evaluate diagnostic utility simultaneously quantifying a large number steroids using liquid chromatography/tandem mass spectrometry (LC-MS/MS) from small serum volume patients with CAH,...
Abstract Polycystic ovary syndrome (PCOS) is characterized by an oligo-anovulation, hyperandrogenism and polycystic ovarian morphology combined with major metabolic disturbances. However, despite the high prevalence human economic consequences of this syndrome, its etiology remains unknown. In study, we show that female Goto-Kakizaki (GK) rats, a type 2 diabetes mellitus model, encapsulate naturally all reproductive hallmarks lean women PCOS at puberty in adulthood. The analysis their...
Reshaping the intestinal microbiota by ingestion of fiber, such as pectin, improves alcohol-induced liver lesions in mice modulating bacterial metabolites, including indoles, well bile acids (BAs). In this context, we aimed to elucidate how oral supplementation pectin affects BA metabolism alcohol-challenged receiving feces from patients with alcoholic hepatitis. Pectin reduced alcohol disease. This beneficial effect correlated lower levels plasma and but higher caecum, suggesting that...
Protein membrane transduction domains that are able to cross the plasma present in several transcription factors, such as homeodomain proteins and viral Tat of HIV-1. Their discovery resulted both new concepts on cell communication during development, conception penetrating peptide vectors for internalisation active molecules into cells. A promising is Penetratin, which crosses membranes by a receptor metabolic energy-independent mechanism. Recent works have claimed Penetratin similar...
Amyotrophic Lateral Sclerosis is an adult-onset neurodegenerative disease characterized by the specific loss of motor neurons, leading to muscle paralysis and death. Although cellular mechanisms underlying ALS-induced toxicity for neurons remain poorly understood, growing evidence suggest a defective energetic metabolism in skeletal muscles participating neuron death ultimately destabilizing neuromuscular junctions. In present study, we report that exercise paradigm, based on high intensity...
Abstract The local immune-inflammatory response elicited by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is still poorly described, as well the extent to which its characteristics may be associated with outcome of critical Coronavirus disease 2019 (COVID-19). In this prospective monocenter study, all consecutive COVID-19 critically ill patients admitted from February December 2020 and explored fiberoptic bronchoscopy bronchoalveolar lavage (BAL) were included....
Obese patientss with nonalcoholic steatohepatitis (NASH) are particularly prone to developing severe forms of coronavirus disease 19 (COVID-19). The gut-to-lung axis is critical during viral infections the respiratory tract, and a change in gut microbiota's composition might have role severity. Here, we investigated consequences infection acute syndrome 2 (SARS-CoV-2) on microbiota context obesity NASH. To this end, set up nutritional model dyslipidemia NASH golden hamster, relevant...
Tryptophan, an essential amino acid, and its metabolites are involved in many physiological processes including neuronal functions, immune system, gut homeostasis. Alterations to tryptophan metabolism associated with various pathologies such as neurologic, psychiatric disorders, inflammatory bowel diseases (IBD), metabolic cancer. It is consequently critical develop a reliable, quantitative method for the analysis of downstream from kynurenine, serotonin, indoles pathways. An LC-MS/MS was...
Testicular sperm extraction (TESE) is an essential therapeutic tool for the management of male infertility. However, it invasive procedure with a success rate up to 50%. To date, no model based on clinical and laboratory parameters sufficiently powerful accurately predict retrieval in TESE. The aim this study compare wide range predictive models under similar conditions TESE outcomes patients nonobstructive azoospermia (NOA) identify correct mathematical approach apply, most appropriate...
Summary Background Bile acid diarrhoea is often missed because gold standard nuclear medicine tauroselcholic [75‐Se] (SeHCAT) testing has limited availability. Empirical treatment effect unknown diagnostic performance, whereas plasma 7α‐hydroxy‐4‐cholesten‐3‐one (C4) inexpensive but lacks sensitivity. Aims To determine characteristics of empirical and explore improvements in diagnostics with potential better availability than SeHCAT. Methods This accuracy study was part a randomised,...
Abstract Background Antibiotics notoriously perturb the gut microbiota. We treated healthy volunteers either with cefotaxime or ceftriaxone for 3 days, and collected in each subject 12 faecal samples up to day 90. Using untargeted targeted phenotypic genotypic approaches, we studied changes bacterial, phage fungal components of microbiota as well metabolome β-lactamase activity stools. This allowed assessing their degrees perturbation resilience. Results While only two subjects had...