A5064491863- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Plant nutrient uptake and metabolism
- Plant Reproductive Biology
- Chromosomal and Genetic Variations
- DNA Repair Mechanisms
- Plant Gene Expression Analysis
- Vibrio bacteria research studies
- Plant Disease Resistance and Genetics
- Gut microbiota and health
- Plant-Microbe Interactions and Immunity
- Plant Pathogens and Fungal Diseases
- Liver Disease Diagnosis and Treatment
- Redox biology and oxidative stress
- Plant Virus Research Studies
- Mitochondrial Function and Pathology
- Phosphodiesterase function and regulation
- Bacillus and Francisella bacterial research
- Inhalation and Respiratory Drug Delivery
- Clostridium difficile and Clostridium perfringens research
- Chromatin Remodeling and Cancer
- Nanopore and Nanochannel Transport Studies
- Glutathione Transferases and Polymorphisms
- Escherichia coli research studies
- Renin-Angiotensin System Studies
Université Paris-Saclay
2013-2025
Inserm
2022-2025
Centre National de la Recherche Scientifique
2008-2025
Institut des Sciences des Plantes de Paris Saclay
2003-2022
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2022
Université Paris Cité
2009-2022
Université Paris-Sud
2006-2020
Sorbonne Paris Cité
2016-2020
Sorbonne Université
2017
Polyploidy is ubiquitous in eukaryotic plant and fungal lineages, it leads to the co-existence of several copies similar or related genomes one nucleus. In plants, polyploidy considered a major factor successful domestication. However, challenges chromosome folding architecture nucleus establish functional structures.
Histone acetyltransferases, which are able to acetylate histone and non-histone proteins, play important roles in gene regulation. Many acetyltransferases related yeast Gcn5, a component of two transcription regulatory complexes SAGA ADA. In this work, by characterizing mutation the Arabidopsis GCN5 (AtGCN5) we studied function controlling floral meristem activity. We show that addition pleiotropic effects on plant development, also leads production terminal flowers. The flowers homeotic...
Although it is a crucial cellular process required for both normal development and to face stress conditions, the control of programmed cell death in plants not fully understood. We previously reported isolation ATXR5 ATXR6, two PCNA-binding proteins that could be involved regulation cycle or death. A yeast two-hybrid screen using as bait captured AtIPS1, an enzyme which catalyses committed step myo-inositol (MI) biosynthesis. atips1 mutants form spontaneous lesions on leaves, raising...
Histone acetyltransferases (HATs) play critical roles in the regulation of chromatin structure and gene expression. Arabidopsis genome contains 12 HAT genes, but biological functions many them are still unknown. In this work, we studied evolutionary relationship cellular two genes homologous to MYST family members.An extensive phylogenetic analysis 105 proteins revealed that they can be divided into 5 classes, each which a specific combination protein modules. The proteins, HAM1 HAM2, belong...
SWI/SNF complexes mediate ATP-dependent chromatin remodeling to regulate gene expression. Many components of these are evolutionarily conserved, and several subunits Arabidopsis thaliana involved in the control flowering, a process that depends on floral repressor FLOWERING LOCUS C (FLC). BAF60 is subunit, this work, we show BAF60, via direct targeting FLC, induces change at high-order level represses photoperiod flowering pathway Arabidopsis. accumulates nucleus controls formation FLC loop...
The proliferating cell nuclear antigen (PCNA) functions as a sliding clamp for DNA polymerase, and is thus key actor in replication. It also involved repair, maintenance of heterochromatic regions throughout replication, cycle regulation programmed death. Identification PCNA partners therefore necessary understanding these processes. Here we identify two Arabidopsis SET-domain proteins that interact with PCNA: ATXR5 ATXR6. A truncated ATXR5Deltaex2, incapable interacting PCNA, occurs planta....
Cotranslational and posttranslational modifications are increasingly recognized as important in the regulation of numerous essential cellular functions. N-myristoylation is a lipid modification ensuring proper function intracellular trafficking proteins involved many signaling pathways. Arabidopsis thaliana, like human, has two tightly regulated N-myristoyltransferase (NMT) genes, NMT1 NMT2. Characterization knockout mutants showed that was strictly required for plant viability, whereas NMT2...
Reshaping the intestinal microbiota by ingestion of fiber, such as pectin, improves alcohol-induced liver lesions in mice modulating bacterial metabolites, including indoles, well bile acids (BAs). In this context, we aimed to elucidate how oral supplementation pectin affects BA metabolism alcohol-challenged receiving feces from patients with alcoholic hepatitis. Pectin reduced alcohol disease. This beneficial effect correlated lower levels plasma and but higher caecum, suggesting that...
The stromal interaction molecules (STIMs) are the sarcoplasmic reticulum (SR) Ca2+ sensors that trigger store-operated entry (SOCE) in a variety of cell types. While STIM1 isoform has been focus research cardiac pathophysiology, function homolog STIM2 remains unknown. Using imaging and patch-clamp techniques, we showed knockdown (KD) by siRNAs increased SOCE ISOC current neonatal rat ventricular cardiomyocytes (NRVMs). Within this cardiomyocyte model, identified transcript expression Stim2.1...
CDC45 is required for the initiation of DNA replication in yeast and cell proliferation mammals functions as a polymerase alpha loading factor Xenopus. We have cloned homolog from Arabidopsis whose expression upregulated at G1/S transition young meiotic flower buds. One-third 35S:CDC45 T1 RNA interference lines are partially to completely sterile, proportion sterile plants increased by using dmc1 promoter. decreased levels transcript contain 21- 23-bp fragments specific gene. T2 transgenic...
* Minichromosome maintenance (MCM) proteins are subunits of the pre-replication complex that probably function as DNA helicases during S phase cell cycle. Here, we investigated AtMCM2 in Arabidopsis. To gain an insight into AtMCM2, combined loss- and gain-of-function approaches. this end, analysed two null alleles generated transgenic plants expressing downstream constitutive 35S promoter. Disruption is lethal at a very early stage embryogenesis, whereas its over-expression results reduced...
Arabidopsis thaliana SNF1-related-kinase 1 (SnRK1)-activating kinase (AtSnAK1) and AtSnAK2 have been shown to phosphorylate in vitro activate the energy signalling integrator, SnRK1. To clarify this cascade planta, a genetic- molecular-based approach was developed. Homozygous single AtSnAK1 T-DNA insertional mutants did not display an apparent phenotype. Crossing of allow isolation double-mutant plants, whereas self-pollinating S1-/- S2+/- sesquimutant specifically gave approximatively 22%...
Bile-acid metabolism and the intestinal microbiota are impaired in alcohol-related liver disease. Activation of bile-acid receptor TGR5 (or GPBAR1) controls both biliary homeostasis inflammatory processes. We examined role alcohol-induced injury mice.We used TGR5-deficient (TGR5-KO) wild-type (WT) female mice, fed alcohol or not, to study involvement macrophages, (16S sequencing), profiles (high-performance liquid chromatography coupled tandem mass spectrometry). Hepatic triglyceride...
BACKGROUND: Pulmonary hypertension is a severe disease with high mortality rates due to right ventricular (RV) failure. The molecular and cellular processes involved in RV remodeling, including Ca 2+ handling, remain elusive the lack of relevant animal models. In this study, we aim understand better pathophysiological mechanisms METHODS: We used chronic thromboembolic pulmonary (CTEPH) pig model, which leads progressive hypertrophy dysfunction. Cellular, unbiased global transcriptional...
Abstract The majority of research on cell cycle regulation is focused the nuclear events that govern replication and segregation genome between two daughter cells. However, eukaryotic cells contain several compartmentalized organelles with specialized functions, coordination among these required for proper progression, as evidenced by isolation mutants in which both organelle function overall plant development were affected. To investigate how chloroplast dysfunction affects cycle, we...
Trichoderma gamsii T6085 has been investigated for many years as a beneficial isolate use in the biocontrol of Fusarium head blight (FHB) wheat caused primarily by graminearum. Previous work focused on application to spikes at anthesis, whereas soil before or sowing received limited attention. In present study, competitive ability plant residues against F. graminearum was investigated. Results showed significant reduction straw colonization pathogen and development perithecia, not only when...
• Premise of the Study: Zygomorphy has evolved multiple times in angiosperms. Near‐actinomorphy is ancestral state early diverging eudicot family Papaveraceae. once subfamily Fumarioideae from a disymmetric state. Unusual within angiosperms, zygomorphy takes place along transverse plane flower. Methods: We investigated floral development to understand developmental bases evolution symmetry then assessed expression candidate genes for key events responsible shift disymmetry zygomorphy, namely...
Faithful transmission of the genetic information is essential in all living organisms. DNA replication therefore a critical step cell proliferation, because potential occurrence errors or damage when progression fork hampered causing replicative stress. Like other types damage, stress activates response, signaling cascade allowing cycle arrest and repair lesions. The polymerase ε (Pol ε) was shown to activate S-phase checkpoint yeast response stress, but whether this mechanism functions...
Chromatin architecture determines transcriptional accessibility to DNA and consequently gene expression levels in response developmental environmental stimuli. Recently, chromatin remodelers such as SWI/SNF complexes have been recognized key regulators of architecture. To gain insight into the function these during root development, we analyzed Arabidopsis knock-down lines for one sub-unit complexes: BAF60. Here, show that BAF60 is a positive regulator development cell cycle progression...
Deoxyuridine triphosphatase (dUTPase) enzyme is an essential that protects DNA against uracil incorporation. No organism can tolerate the absence of this activity. In article, we show dUTPase function conserved between E. coli (Escherichia coli), yeast (Saccharomyces cerevisiae) and Arabidopsis (Arabidopsis thaliana) it in as both micro-organisms. Using a RNA interference strategy, plant lines were generated with diminished activity compared to wild-type. These plants are sensitive...
Meristems retain the ability to divide throughout life cycle of plants, which can last for over 1000 years in some species. Furthermore, germline is not laid down early during embryogenesis but originates from meristematic cells relatively late development. Thus, accurate cell regulation utmost importance avoid accumulation mutations vegetative growth and reproduction. The Arabidopsis thaliana genome encodes two homologs replication licensing factor CDC10 Target1 (CDT1), overexpression CDT1a...
Faithful DNA replication maintains genome stability in dividing cells and from one generation to the next. This is particularly important plants because whole plant body reproductive originate meristematic that retain their proliferative capacity throughout life cycle of organism. involves large sets proteins whose activity strictly regulated, tightly linked damage response detect respond errors or defects. Central this interconnection replicative polymerase Polymerase ϵ (Pol ϵ) which...