A5033622862- Photosynthetic Processes and Mechanisms
- Plant nutrient uptake and metabolism
- Plant Stress Responses and Tolerance
- Legume Nitrogen Fixing Symbiosis
- Plant Molecular Biology Research
- Plant-Microbe Interactions and Immunity
- Mitochondrial Function and Pathology
- Biochemical and Molecular Research
- Plant Parasitism and Resistance
- Microbial Natural Products and Biosynthesis
- Nematode management and characterization studies
- Metabolomics and Mass Spectrometry Studies
- Allelopathy and phytotoxic interactions
- Mycotoxins in Agriculture and Food
- Microbial Metabolites in Food Biotechnology
- Wheat and Barley Genetics and Pathology
- Plant Physiology and Cultivation Studies
- Enzyme Structure and Function
- Light effects on plants
- Plant Pathogens and Fungal Diseases
- RNA modifications and cancer
- Mass Spectrometry Techniques and Applications
- Genomics and Phylogenetic Studies
- Plant Water Relations and Carbon Dynamics
- Metal-Catalyzed Oxygenation Mechanisms
Centre National de la Recherche Scientifique
2011-2024
Institut des Sciences des Plantes de Paris Saclay
2014-2024
Université Paris-Saclay
2018-2024
Université Paris Cité
2011-2023
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2023
Université Paris-Sud
2009-2022
Sorbonne Paris Cité
2018-2021
Sorbonne Université
2018
Laboratoire Physiologie Cellulaire & Végétale
2011
Institut Universitaire de France
2011
Several plant species require microbial associations for survival under different biotic and abiotic stresses. In this study, we show that Enterobacter sp. SA187, a desert endophytic bacterium, enhances yield of the crop alfalfa field conditions as well growth model Arabidopsis thaliana in vitro, revealing high potential SA187 biological solution improving production. Studying interaction with Arabidopsis, uncovered number mechanisms related to beneficial association plants. colonizes both...
Summary Elevated ambient temperatures affect plant growth and substantially impact biomass crop yield. Recent results have indicated that chromatin remodelling is critical in thermal responses but how histone modification dynamics affects response has not been clearly demonstarted. Here we show Arabidopsis deacetylase genes HDA 9 , 15 19 play distinct roles to elevated temperature. hda9 hda19 mutants showed a warm‐temperature‐insensitive phenotype at 27°C, whereas hda15 plants displayed...
Summary Plant development and function are underpinned by redox reactions that depend on co‐factors such as nicotinamide adenine dinucleotide (NAD). NAD has recently been shown to be involved in several signalling pathways associated with stress tolerance or defence responses. However, the mechanisms which influences plant gene regulation, metabolism physiology still remain unclear. Here, we took advantage of Arabidopsis thaliana lines overexpressed nadC from E. coli , encodes biosynthesis...
Although there is now a considerable literature on the inhibition of leaf respiration (CO(2) evolution) by light, little known about effect other environmental conditions day respiratory metabolism. In particular, CO(2) and O(2) mole fractions are assumed to cause changes in tricarboxylic acid pathway (TCAP) but amplitude even direction such still matter debate. Here, we took advantage isotopic techniques, new simple equations instant freeze sampling follow metabolism illuminated cocklebur...
Summary Uridine nucleotides can be formed by energy‐consuming de novo synthesis or the energy‐saving recycling of nucleobases resulting from nucleotide catabolism. Uracil phosphoribosyltransferases (UPRTs; EC 2.4.2.9) are involved in salvage pyrimidines catalyzing formation uridine monophosphate (UMP) uracil and phosphoribosylpyrophosphate. To date, UPRTs described as non‐essential, enzymes. In present work, six genes annotated Arabidopsis genome examined through phylogenetic functional...
Abstract The photorespiratory cycle is a crucial pathway in photosynthetic organisms because it removes toxic 2‐phosphoglycolate made by the oxygenase activity of ribulose‐1,5‐bisphosphate carboxylase/oxygenase and retrieves its carbon as 3‐phosphoglycerate. Mitochondrial serine hydroxymethyltransferase 1 (SHMT1) an essential enzyme converting glycine to serine. SHMT1 regulation remains poorly understood although could involve phosphorylation 31. Here, we report complementation Arabidopsis...
Abstract Plants have fundamental dependences on nitrogen and sulfur frequently to cope with chronic limitations when their supply is sub-optimal. This study aimed at characterizing the metabolomic, proteomic, transcriptomic changes occurring in Arabidopsis leaves under nitrate (Low-N) sulfate (Low-S) order compare effects, determine interconnections, examine strategies of adaptation. Metabolite profiling globally revealed opposite effects Low-S Low-N carbohydrate amino acid accumulations,...
In Angiosperms, the development of vascular system is controlled by a complex network transcription factors. However, how nutrient availability in cells affects their remains to be addressed. At cellular level, cytosolic sugar regulated mainly exchanges at tonoplast through active and/or facilitated transport. Arabidopsis (Arabidopsis thaliana), among genes encoding tonoplastic transporters, SUGAR WILL EVENTUALLY BE EXPORTED TRANSPORTER 16 (SWEET16) and SWEET17 expression has been previously...
Abstract Manganese (Mn) is an essential metal for plant growth. The most important Mn-containing enzyme the Mn4CaO5 cluster that catalyzes water oxidation in photosystem II (PSII). Mn deficiency primarily affects photosynthesis, whereas excess generally toxic. Here, we studied and liverwort Marchantia polymorpha, emerging model ideally suited analysis of stress since it accumulates rapidly toxic substances due to absence well-developed vascular radicular systems a reduced cuticle. We...
Abstract The natural 13 C/ 12 C isotope composition ( δ C) of plants and organic compounds within plant organs is a powerful tool to understand carbon allocation patterns the regulation photosynthetic or respiratory metabolism. However, many enzymatic fractionations are currently unknown, thus impeding our understanding trafficking pathways cells. One them effect associated with invertases (EC 3.2.1.26) that cornerstone enzymes for Suc metabolism translocation in plants. Another conundrum...
A valuable strategy to improve crop yield consists in the use of plant growth-promoting rhizobacteria (PGPRs). However, influence PGPR colonization on physiology is largely unknown. Burkholderia phytofirmans strain PsJN (Bp PsJN) colonized only Arabidopsis thaliana roots after seed or soil inoculation. Foliar bacteria were detected leaf infiltration. Since, different bacterial times presence and/or locations host could lead physiological responses, photosynthesis, and metabolite profiles A....
Abstract Chassis strains, derived from Streptomyces coelicolor M145, deleted for one or more of its four main specialized metabolites biosynthetic pathways (CPK, CDA, RED and ACT), in various combinations, were constructed the heterologous expression types origins. To determine consequences these deletions on metabolism strains comparative lipidomic metabolomic analyses original strain carried out. These studies unexpectedly revealed that deletion peptidic clusters, and/or a ACT cluster,...
Deoxyuridine triphosphatase (dUTPase) enzyme is an essential that protects DNA against uracil incorporation. No organism can tolerate the absence of this activity. In article, we show dUTPase function conserved between E. coli (Escherichia coli), yeast (Saccharomyces cerevisiae) and Arabidopsis (Arabidopsis thaliana) it in as both micro-organisms. Using a RNA interference strategy, plant lines were generated with diminished activity compared to wild-type. These plants are sensitive...
Plant uridine diphosphate (UDP)-glucosyltransferases (UGT) catalyze the glucosylation of xenobiotic, endogenous substrates and phytotoxic agents produced by pathogens such as mycotoxins. The Bradi5g03300 UGT-encoding gene from model plant Brachypodium distachyon was previously shown to confer tolerance mycotoxin deoxynivalenol (DON) through into DON 3-O-glucose (D3G). This be involved in early establishment quantitative resistance Fusarium Head Blight, a major disease small-grain cereals. In...
Plant mutants for genes encoding subunits of mitochondrial complex I (CI; NADH:ubiquinone oxidoreductase), the first enzyme respiratory chain, display various phenotypes depending on growth conditions. Here, we examined impact photoperiod, a major environmental factor controlling plant development, two Arabidopsis (Arabidopsis thaliana) CI mutants: new insertion mutant interrupted in both ndufs8.1 and ndufs8.2 NDUFS8 subunit previously characterized ndufs4 mutant. In long day (LD) condition,...
Abstract Day respiration is the cornerstone of nitrogen assimilation since it provides carbon skeletons to primary metabolism for glutamate (Glu) and glutamine synthesis. However, recent studies have suggested that tricarboxylic acid pathway rate limiting mitochondrial pyruvate dehydrogenation partly inhibited in light. Pyruvate may serve as a source amino (e.g. alanine) or fatty synthesis, but not well documented, neither possible resynthesis phosphoenolpyruvate (PEP). Here, we examined...
Photorespiration is an essential process in oxygenic photosynthetic organisms triggered by the oxygenase activity of Rubisco. In peroxisomes, photorespiratory HYDROXYPYRUVATE REDUCTASE1 (HPR1) catalyzes conversion hydroxypyruvate to glycerate together with oxidation a pyridine nucleotide cofactor. HPR1 regulation remains poorly understood; however, phosphorylation at T335 has been reported. By comparing kinetic properties phosphomimetic (T335D), nonphosphorylatable (T335A), and wild-type...
Many horticultural and natural plant species have variegated leaves, that is, patchy leaves with green non-green or white areas. Specific studies on the metabolism of are scarce although (non-green) areas been assumed to play role a 'nitrogen store', there is no specific showing analysis nitrogenous metabolites dynamics nitrogen assimilation. Here, we examined Pelargonium×hortorum. We show larger N:C ratio, more amino acids, clear accumulation arginine. Metabolomic analyses revealed...
ATP wasting is recognized as an efficient strategy to enhance metabolic activity and productivity of specific metabolites in several microorganisms . However, such has been rarely implemented Streptomyces species whereas antibiotic production by members this genus known be triggered condition phosphate limitation that correlated with a low content. In consequence, assess the effects spilling on primary specialized metabolisms , gene encoding small synthetic protein DX, high affinity for...
Legume-rhizobium symbiosis is a major ecological process in the nitrogen cycle, responsible for main input of fixed into biosphere. The efficiency this relies on coevolution partners.
Legume-rhizobium symbiosis is a major ecological process, fueling the biogeochemical nitrogen cycle with reduced nitrogen. It also represents promising strategy to reduce use of chemical fertilizers in agriculture, thereby improving its sustainability. This interaction leads intracellular accommodation rhizobia within plant cells symbiotic organs, where they differentiate into nitrogen-fixing bacteroids. In specific legume clades, this differentiation process requires bacterial transporter...
Application of metabolomics techniques to plant physiology is now considerable, and LC-MS often being used for non-targeted, semi-quantitative analysis effects caused by mutations or environmental conditions. However, examination signalling metabolites like hormones require absolute rather than quantitation, since their effect in planta strongly dependent upon concentration. Further, belong different chemical classes thus simultaneous quantitation remains highly challenging. Here we present...
Abstract Maize is currently the most productive cereal crop in world ( www.faostat.org ). can form a symbiotic relationship with Arbuscular Mycorrhizal Fungus (AMF), Rhizophagus irregularis . In this relationship, fungus provides plant additional water and mineral nutrients, while supplies carbon compounds to fungus. Little known about N metabolism disruption during symbiosis both partners. To address issue, two genetically distant maize lines were studied terms of physiological molecular...