A5055044216- Plant-Microbe Interactions and Immunity
- Plant Stress Responses and Tolerance
- Plant Molecular Biology Research
- Plant Pathogenic Bacteria Studies
- Photosynthetic Processes and Mechanisms
- Plant tissue culture and regeneration
- Plant nutrient uptake and metabolism
- Plant Parasitism and Resistance
- Transgenic Plants and Applications
- Plant Gene Expression Analysis
- Plant and Biological Electrophysiology Studies
- Seed Germination and Physiology
- Ion channel regulation and function
- Plant pathogens and resistance mechanisms
- Plant Reproductive Biology
- Fungal and yeast genetics research
- Insect-Plant Interactions and Control
- Aluminum toxicity and tolerance in plants and animals
- Plant Virus Research Studies
- Polysaccharides and Plant Cell Walls
- GABA and Rice Research
- Fungal Biology and Applications
- Agriculture Sustainability and Environmental Impact
- Photoreceptor and optogenetics research
- Genomics, phytochemicals, and oxidative stress
Institut des Sciences des Plantes de Paris Saclay
2016-2025
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2025
Centre National de la Recherche Scientifique
2016-2025
Université Paris-Saclay
2017-2025
Université Paris Cité
2015-2024
Université Paris-Sud
2015-2019
Sorbonne Paris Cité
2017-2019
Centre National pour la Recherche Scientifique et Technique (CNRST)
2019
Sorbonne Université
2017-2018
Institut National de la Recherche Agronomique
2015
The development and morphology of vascular plants is critically determined by synthesis proper distribution the phytohormone auxin. directed cell-to-cell auxin achieved through a system influx efflux transporters. PIN-FORMED (PIN) proteins are proposed transporters, fluxes can seemingly be predicted based on the--in many cells--asymmetric plasma membrane PINs. Here, we show in heterologous Xenopus oocyte as well Arabidopsis thaliana inflorescence stems that PIN-mediated transport directly...
Summary Abscisic acid ( ABA ) is a major phytohormone involved in important stress‐related and developmental plant processes. Recent phosphoproteomic analyses revealed large set of ‐triggered phosphoproteins as putative mitogen‐activated protein kinase MAPK targets, although the evidence for s signalling still scarce. Here, we identified reconstituted vivo complete ‐activated cascade, composed MAP 3Ks 3K17/18, 2K MKK 3 four C group MPK 1/2/7/14. In planta , show that activation 7 blocked...
Plant mitogen-activated protein kinases (MAPKs) are involved in important processes, including stress signaling and development. In a functional yeast screen, we identified mutations that render Arabidopsis thaliana MAPKs constitutively active (CA). Importantly, CA-MAPKs maintain their specificity toward known activators substrates. As proof-of-concept, MAPK4 (MPK4) function plant immunity was investigated. agreement with the phenotype of mpk4 mutants, CA-MPK4 plants were compromised...
Abstract Cytokinesis in plants is achieved by the formation of cell plate. A pathway that includes mitogen-activated protein (MAP) kinase and MAP (MAPKK) plays a key role control plant cytokinesis. We show here kinase, MPK4, required for plate Arabidopsis thaliana. Single mutations MPK4 caused dwarfism characteristic defects cytokinesis, such as immature plates, which became much more prominent upon introduction mutation MKK6/ANQ, MAPKK into mpk4. MKK6/ANQ strongly activated protoplasts,...
Abstract Background Mitogen-activated protein kinases (MAPKs) are key regulators of immune responses in animals and plants. In Arabidopsis , perception microbe-associated molecular patterns (MAMPs) activates the MAPKs MPK3, MPK4 MPK6. Increasing information depicts events activated by MAMPs plants, but specific cooperative contributions these signalling largely unclear. Results this work, we analyse behaviour MPK3 MPK6 mutants early late triggered MAMP flg22 from bacterial flagellin. A...
Several plant species require microbial associations for survival under different biotic and abiotic stresses. In this study, we show that Enterobacter sp. SA187, a desert endophytic bacterium, enhances yield of the crop alfalfa field conditions as well growth model Arabidopsis thaliana in vitro, revealing high potential SA187 biological solution improving production. Studying interaction with Arabidopsis, uncovered number mechanisms related to beneficial association plants. colonizes both...
Abiotic and biotic factors cause plant wounding trigger complex short- long-term responses at the local systemic levels. These are under control of signaling pathways, which still poorly understood. Here, we show that rapid activation clade-A mitogen-activated protein kinases (MAPKs) MPK3 MPK6 by depends on upstream MAPK MKK4 MKK5 but is independent jasmonic acid (JA) signaling. In addition, this fast module does not wound-triggered JA accumulation in Arabidopsis (Arabidopsis thaliana),...
Four hundred and fifty-eight genes coding for PentatricoPeptide Repeat (PPR) proteins are annotated in the Arabidopsis thaliana genome. Over past 10 years, numerous reports have shown that many of these function organelles to target specific transcripts involved post-transcriptional regulation. Therefore, they thought be important players coordination between nuclear organelle genome expression. Only four been described addressed outside organelles, indicating some PPRs could regulations...
Microbial-associated molecular patterns activate several MAP kinases, which are major regulators of the innate immune response in Arabidopsis thaliana that induce large-scale changes gene expression. Here, we determine whether microbial-associated pattern-triggered expression involves modifications at chromatin level. Histone acetylation and deacetylation implicate histone deacetylase HD2B reprogramming defence immunity. The kinase MPK3 directly interacts with phosphorylates HD2B, thereby...
Abstract Arabidopsis thaliana mitogen-activated protein kinases 3 and 6 (MPK3/6) are activated transiently during pathogen-associated molecular pattern-triggered immunity (PTI) durably effector-triggered (ETI). The functional differences between these two kinds of activation kinetics how they coordinate the layers plant remain poorly understood. Here, by suppressor analyses, we demonstrate that ETI-mediating nucleotide-binding domain leucine-rich repeat receptors (NLRs) NLR signaling...
Mitogen-activated protein kinases (MAPKs) are important regulators of plant immunity. Most the knowledge about function these pathways is derived from loss-of-function approaches. Using a gain-of-function approach, we investigated responses controlled by constitutively active (CA) MPK3 in Arabidopsis thaliana. CA-MPK3 plants dwarfed and display massive derepression defense genes associated with spontaneous cell death as well accumulation reactive oxygen species, phytoalexins, stress-related...
Plant defense responses involve several biological processes that allow plants to fight against pathogenic attacks. How these different are orchestrated within organs and depend on specific cell types is poorly known. Here, using single-cell RNA sequencing (scRNA-seq) technology three independent replicates, we identified populations representing the core transcriptional of wild-type Arabidopsis leaves inoculated with bacterial pathogen Pseudomonas syringae DC3000. Among populations,...
SUMMARY Plant responses to nutrient availability are critical for plant development and yield. Nitrate, the major form of nitrogen in most soils, serves as both a signaling molecule. Nitrate itself triggers rapid, changes gene expression, especially via nodule inception (NIN)‐like protein (NLP) transcription factors, stimulates phosphorylation. Mitogen‐activated kinase (MAPK)‐related genes among early nitrate‐responsive genes; however, little is known about their roles nitrate pathways....
Abstract On the basis of anion content in vitro-cultured Arabidopsis plantlets, we explored selectivity voltage-dependent channel plasma membrane hypocotyl cells. In whole-cell configuration, substitution cytosolic Cl− by different anions led to following sequence relative permeabilities: NO3 − (2.6) ≥ SO4 2− (2.0) > Cl−(1.0) HCO3 (0.8) ≫ malate2− (0.03). Large currents were measured for and 2−, about five six times higher than equivalent Cl−currents. Since is usually considered be a...
Phospholipase D alpha 1 (PLDα1, At3g15730) and its product phosphatidic acid (PA) are involved in a variety of cellular physiological processes, such as cytoskeletal remodelling, regulation stomatal closure opening, well biotic abiotic stress signalling. Here we aimed to study developmental expression patterns subcellular localization PLDα1 Arabidopsis using advanced microscopy methods light-sheet fluorescence (LSFM) structured illumination (SIM). We complemented two knockout pldα1 mutants...