A5031816827- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Plant Reproductive Biology
- Microtubule and mitosis dynamics
- Legume Nitrogen Fixing Symbiosis
- Plant tissue culture and regeneration
- Photosynthetic Processes and Mechanisms
- Agronomic Practices and Intercropping Systems
- Polysaccharides and Plant Cell Walls
- Transgenic Plants and Applications
- Sports and Physical Education Research
- Sports Performance and Training
- Advanced Fluorescence Microscopy Techniques
- Microbial Metabolites in Food Biotechnology
- Cellular Mechanics and Interactions
- Physical Education and Training Studies
- Lysosomal Storage Disorders Research
- Plant Stress Responses and Tolerance
Palacký University Olomouc
2016-2021
Czech Academy of Sciences, Biology Centre
2016-2020
Czech Academy of Sciences, Institute of Microbiology
1991
Phospholipase D alpha 1 (PLDα1, At3g15730) and its product phosphatidic acid (PA) are involved in a variety of cellular physiological processes, such as cytoskeletal remodelling, regulation stomatal closure opening, well biotic abiotic stress signalling. Here we aimed to study developmental expression patterns subcellular localization PLDα1 Arabidopsis using advanced microscopy methods light-sheet fluorescence (LSFM) structured illumination (SIM). We complemented two knockout pldα1 mutants...
The development of the root apex is determined by progress cells from meristematic region to successive post-mitotic developmental zones for transition, cell elongation and final differentiation. We addressed development, tissue architecture zonation means light-sheet microscopic imaging Arabidopsis thaliana seedlings expressing END BINDING protein 1c (EB1c) fused green fluorescent (GFP) under control native EB1c promoter. Unlike other two members EB1 family, plant-specific shows prominent...
Phospholipase D alpha 1 (PLDα1, AT3G15730) and mitogen-activated protein kinases (MAPKs) participate on signaling-dependent events in plants. MAPKs are able to phosphorylate a wide range of substrates putatively including PLDs. Here we have focused functional regulations PLDα1 by interactions with MAPKs, their co-localization impact salt stress abscisic acid (ABA) tolerance Arabidopsis. Yeast two-hybrid bimolecular fluorescent assays showed that interacts MPK3. Immunoblotting analyses...
The phragmoplast separates daughter cells during cytokinesis by constructing the cell plate, which depends on interaction between cytoskeleton and membrane compartments. Proteins responsible for these interactions remain unknown, but formins can link with membranes several members of formin protein family localize to plate. Progress in functional characterization is hindered redundancies within large gene family. We addressed this limitation employing Small Molecular Inhibitor Formin...
Nitrogen-fixing rhizobia and legumes have developed complex mutualistic mechanism that allows to convert atmospheric nitrogen into ammonia. Signalling by mitogen-activated protein kinases (MAPKs) seems be involved in this symbiotic interaction. Previously, we reported stress-induced MAPK (SIMK) shows predominantly nuclear localization alfalfa root epidermal cells. Nevertheless, SIMK is activated relocalized the tips of growing hairs during their development. kinase (SIMKK) a well-known...