A5040767007- Cardiomyopathy and Myosin Studies
- Cellular Mechanics and Interactions
- Plant-Microbe Interactions and Immunity
- Plant Disease Resistance and Genetics
- Plant Pathogenic Bacteria Studies
- Cardiovascular Effects of Exercise
- Plant Virus Research Studies
- Plant Pathogens and Resistance
- Neurogenetic and Muscular Disorders Research
- RNA Research and Splicing
- Muscle Physiology and Disorders
- Economic Issues in Ukraine
- Bacteriophages and microbial interactions
- Plant Pathogens and Fungal Diseases
- Microtubule and mitosis dynamics
- Mitochondrial Function and Pathology
- Enterprise Management and Information Systems
- Cancer, Hypoxia, and Metabolism
- Fungal Plant Pathogen Control
- Plant Molecular Biology Research
- Sustainability and Innovation in Business
- Viral Infections and Immunology Research
- Signaling Pathways in Disease
- Business and Economic Development
- Banking, Crisis Management, COVID-19 Impact
Washington State University
2014-2025
Lviv University
2018
Children's Hospital at Westmead
2014
Westmead Institute
2014
Ann Arbor VA Medical Center
2014
University of Michigan
2014
Johnson University
2010
Burke Medical Research Institute
2010
Cornell University
2010
Nemaline myopathy (NM) is a genetic muscle disorder characterized by dysfunction and electron-dense protein accumulations (nemaline bodies) in myofibers. Pathogenic mutations have been described 9 genes to date, but the basis remains unknown many cases. Here, using an approach that combined whole-exome sequencing (WES) Sanger sequencing, we identified homozygous or compound heterozygous variants LMOD3 21 patients from 14 families with severe, usually lethal, NM. encodes leiomodin-3 (LMOD3),...
Regulation of actin filament assembly is essential for efficient contractile activity in striated muscle. Leiomodin an actin-binding protein and homolog the pointed-end capping protein, tropomodulin. These proteins are structurally similar, sharing a common domain organization that includes two sites. also contains unique C-terminal extension has third WH2 domain. Recently, striated-muscle-specific isoform leiomodin (Lmod2) was reported to be nucleator cardiomyocytes. Here, we have...
Potato powdery scab, caused by the soil-borne pathogen Spongospora subterranea f. sp. (Sss), poses a significant threat to potato production, reducing value and impacting fresh market quality. Effective management strategies for this disease are currently lacking, Sss is widespread in many potato-growing regions, highlighting urgent need effective control measures. Although use of disease-resistant cultivars holds potential as sustainable solution, genetic mechanisms underlying resistance...
The role of small secreted peptides in plant defense responses to viruses has seldom been investigated. Here, we report a for potato (Solanum tuberosum) PIP1, gene predicted encode member the pathogen-associated molecular pattern (PAMP)-induced peptide (PIP) family, response Potato virus Y (PVY) infection. We show that exogenous application synthetic StPIP1 leaves and nodes increased production reactive oxygen species expression defense-related genes, revealing triggers early responses. In...
ABSTRACT Flagella are extracellular organelles that propel bacteria. Each flagellum consists of a basal body, hook, and filament. The major protein the filament is flagellin. Induction flagellin gene expression coincides with secretion FlgM. role FlgM to inhibit FliA (σ 28 ), flagellum-specific RNA polymerase responsible for transcription. To prevent premature polymerization newly synthesized molecules, FliS, flagellin-specific chaperone, binds facilitates its export. In this study,...
The first layer of plant immunity is deployed by recognition pathogen-associated molecule patterns (PAMPs) and induction early stress responses. Flagellin the major protein component flagellum. Flagellin-derived peptide fragments such as Flg22, a short active derived from highly conserved part N-terminal region, are recognized PAMPs specific perception system present in most higher plants. Some bacteria evade altering Flg22 region flagellin. Instead, small subset plants (i.e., solanaceous...
Leiomodin is a potent actin nucleator related to tropomodulin, capping protein localized at the pointed end of thin filaments. Mutations in leiomodin-3 are associated with lethal nemaline myopathy humans, and leiomodin-2–knockout mice present dilated cardiomyopathy. The arrangement N-terminal actin- tropomyosin-binding sites leiomodin contradictory functionally not well understood. Using one-dimensional nuclear magnetic resonance pointed-end polymerization assay, we find that leiomodin-2,...
A quantitative and robust bioassay to assess plant defense response is important for studies of disease resistance also the early identification during pre- or non-symptomatic phases. An increase in extracellular pH known be an plants. In this study, we demonstrate alkalinization as a potatoes. Using potato suspension cell cultures, observed against various pathogen- plant-derived elicitors dose- time-dependent manner. We assessed variety pathogens, such protists (Phytophthora infestans...
On-site diagnosis of plant diseases can be a useful tool for growers timely decisions enabling the earlier implementation disease management strategies that reduce impact disease. Presently in many diagnostic laboratories, polymerase chain reaction (PCR), particularly real-time PCR, is considered most sensitive and accurate method pathogen detection. However, laboratory-based PCRs typically require expensive laboratory equipment skilled personnel. In this study, soil-borne pathogens potato...
The phragmoplast separates daughter cells during cytokinesis by constructing the cell plate, which depends on interaction between cytoskeleton and membrane compartments. Proteins responsible for these interactions remain unknown, but formins can link with membranes several members of formin protein family localize to plate. Progress in functional characterization is hindered redundancies within large gene family. We addressed this limitation employing Small Molecular Inhibitor Formin...
Assembly of the actin cytoskeleton is an important part formation neurites in developing neurons. Tropomodulin, a tropomyosin-dependent capping protein for pointed end filament, one key players this process. Tropomodulin binds tropomyosin two binding sites. Tmod1 and Tmod2, tropomodulin isoforms found neurons, were overexpressed PC12 cells, model system neuronal differentiation. did not affect differentiation; while cells expressing Tmod2 showed significant reduction number length neurites....
Early detection of causal pathogens is important to prevent crop loss from diseases. However, some diseases, such as soilborne are difficult diagnose due the absence visible or characteristic symptoms. In present study, use Oxford Nanopore MinION sequencer a molecular diagnostic tool was assessed its long-read sequencing capabilities and portability. Nucleotide samples (DNA RNA) potato field soils were sequenced analyzed using locally curated pathogen database, followed by identification via...
Powdery scab is an important potato disease caused by the soilborne pathogen Spongospora subterranea f. sp. subterranea. Currently, reliable chemical control and resistant cultivars for powdery are unavailable. As alternative strategy, we propose a novel approach involving effective delivery of phytocytokine to plant roots rhizobacterium Bacillus subtilis. The modified strain designed secrete elicitor peptide StPep1. In our experiments employing hairy root system, observed significant...
Actin dynamics is fundamental for neurite development; monomer depolymerization from pointed ends rate-limiting in actin treadmilling. Tropomodulins (Tmod) make up a family of end-capping proteins. Of the four known isoforms, Tmod1-Tmod3 are expressed brain cells. We investigated role Tmod's C-terminal (LRR) domain formation neurite-like processes by overexpressing Tmod1 and Tmod2 with deleted or mutated LRR domains PC12 cells, model system used to study neuritogenesis. overexpression...
Abstract Plant recognition of invading organisms occurs through identification foreign molecules associated with attackers and self‐derived, damage‐associated molecules. Perception these activates signaling processes including dynamic changes in ion balance, production second messengers such as reactive oxygen species nitric oxide, increased levels plant hormones, map kinase cascade activation. Together events stimulate transcriptional to initiate defense responses. Among the earliest...
Tropomodulins are a family of important regulators actin dynamics at the pointed ends filaments. Four isoforms tropomodulin, Tmod1-Tmod4, expressed in vertebrates. Binding tropomodulin to end is dependent on tropomyosin, an binding protein that itself represented mammals by up 40 isoforms. The understanding regulatory role tropomodulin/tropomyosin molecular diversity has been limited due lack three-dimensional structure complex. In this study, we mapped tropomyosin residues interacting with...
In agroecosystems, plants frequently confront multiple biotic stressors, including herbivores and pathogens. The nature of these interactions plays a crucial role in mediating the activation plant defense mechanisms. However, induction chemical defenses has been more well studied than physical defenses. Here, we assessed responses pea (Pisum sativum) after exposure to three stressors: vector herbivore (pea aphid, Acrythosiphon pisum), non-vector leaf weevil, Sitona lineatus), virus (Pea...
The article studies the creation and functioning of industrial parks. potential for intensifying development is achieved through mechanism placement parks in areas free from building or already existing require special attention. examines experience on example countries world. A distinctive feature Europe USA their clusters zones with a clearly defined specialization territories economic zones. classification has been investigated. Their features characteristics have determined, which must...
The purpose of this scientific article is to find a complex solution the implementation security audit at enterprise by developing theoretical and practical recommendations based on principles socially oriented economy. It should be focused information provision for system economic enterprise. This deals with creation authors considered basic theory researched its evolution. An analysis current state fight against offenses in sphere was carried out. prospects implementing were determined....