A5031444983- Plant Disease Resistance and Genetics
- Plant Pathogens and Resistance
- Plant Pathogens and Fungal Diseases
- Plant and Fungal Interactions Research
- Plant Disease Management Techniques
- Plant Pathogenic Bacteria Studies
- Fungal Plant Pathogen Control
- Mycotoxins in Agriculture and Food
- Forest Insect Ecology and Management
- Banana Cultivation and Research
- Horticultural and Viticultural Research
- Plant Virus Research Studies
- Nanoparticles: synthesis and applications
- Yeasts and Rust Fungi Studies
- Plant-Microbe Interactions and Immunity
- Nematode management and characterization studies
- Biosensors and Analytical Detection
- Microplastics and Plastic Pollution
- Composting and Vermicomposting Techniques
- Phytochemistry and biological activities of Ficus species
- Interdisciplinary Research and Collaboration
- Eosinophilic Disorders and Syndromes
- Phytoplasmas and Hemiptera pathogens
- Listeria monocytogenes in Food Safety
- Higher Education Governance and Development
University of Idaho
2016-2025
Leeds Beckett University
2023
Fera Science (United Kingdom)
2011-2019
University Hospitals Parma Medical Center
2019
Central Forensic Science Laboratory
2008
Harper Adams University
2004-2008
Species of Diaporthe are considered important plant pathogens, saprobes, and endophytes on a wide range hosts. Several species well-known grapevines, either as agents pre- or post-harvest infections, including Phomopsis cane leaf spot, bleaching, swelling arm trunk cankers. In this study we explore the occurrence, diversity pathogenicity spp. associated with Vitis vinifera in major grape production areas Europe Israel, focusing nurseries vineyards. Surveys were conducted Croatia, Czech...
One hundred and thirty five isolates of Rhizoctonia solani were obtained from British potato crops between 2001 2003. Isolates assigned to anastomosis group (AG) using conventional PCR assays for AG2‐1 or AG3 through the observation hyphal interactions, where appropriate. A previously published primer set was modified in this study enhance specificity AG3PT. Most (92·6%) belonged AG3PT whilst some (6·7%) AG2‐1. Only one isolate recovered (0·7%) AG5. diverse, with variation both length rDNA...
Many species of fungi can cause disease in plants, animals and humans. Accurate robust detection quantification is essential for diagnosis, modeling surveillance. Also direct enables a deeper understanding natural microbial communities, particularly as great many are difficult or impossible to cultivate. In the last decade, effective amplification platforms, probe development various quantitative PCR technologies have revolutionized research on fungal identification. Examples latest...
The Alternaria section alternaria (A. alternata species group) represents a diverse group of saprotroph, human allergens and plant pathogens. taxonomy has benefited from recent phylogenetic revision but the basis differentiation between major clades within is not yet understood. Furthermore, genomic resources have been limited for study host-specific pathotypes. We report near complete genomes apple Asian pear pathotypes as well draft assemblies further 10 isolates representing tenuissima...
Rhizoctonia solani is a plant pathogenic fungus that causes significant establishment and yield losses to several important food crops globally. This the first application of high resolution X-ray micro Computed Tomography (X-ray µCT) real-time PCR study host-pathogen interactions in situ elucidate mechanism damping-off disease over 6-day period caused by R. solani, anastomosis group (AG) 2-1 wheat (Triticum aestivum cv. Gallant) oil seed rape (OSR, Brassica napus Marinka). Temporal,...
A survey of anastomosis groups (AG) Rhizoctonia spp. associated with potato diseases was conducted in South Africa. In total, 112 solani and 19 binucleate (BNR) isolates were recovered from diseased plants, characterized for AG pathogenicity. The identity the confirmed using phylogenetic analysis internal transcribed spacer region ribosomal DNA. R. belonged to 3-PT, 2-2IIIB, 4HG-I, 4HG-III, 5, while BNR R, frequencies 74, 6.1, 2.3, 0.8, 12.2, 2.3%, respectively. 3-PT most predominant...
In August 2023, disease symptoms were noted on mint plants (Mentha arvensis) in two southwest Idaho fields with 10 to 30% of the exhibiting dark brown stem lesions and root necrosis. To identify causal agent, isolations from symptomatic tissue conducted. The surface material was treated sodium hypochlorite (0.6%) for 1 minute washed three times using sterile water. Approximately 2 mm³ sections cultured water agar supplemented 0.02% penicillin 0.08% streptomycin incubated at room temperature...
Glasshouse and field experiments showed that the pathogenicity disease type on potato varied between different anastomosis groups (AGs) of Rhizoctonia solani. For example, severe stem stolon developed in plants inoculated with a single isolate AG3PT AG5. Severe root was observed isolates AG8 to lesser extent AG3PT, but rarely other AGs tested. In both glasshouse AG2‐1 (X81) produced only small lesions (<5 mm). However, this not representative two isolates. When three rDNA IGS1 types were...
Engineered nanoparticles (ENPs) will be released to the environment during use or following disposal of ENP-containing products and concerns have been raised over risks ENPs environment. Many studies explored toxicity aquatic organisms but these usually performed with little understanding ENPs' behaviour in test media relationship between natural waters. This study evaluated compared aggregation four model gold nanoparticle (NP) types (coated neutral, negative, positive amphoteric cappings)...
Summary Kauri A gathis australis, an iconic tree of N ew Z ealand, is under threat from introduced disease‐causing pathogen provisionally named P hytophthora ‘taxon gathis’ (referred to as PTA ). This soilborne, ythiaceous species belongs the C hromista and causes a collar rot resulting in yellowing foliage thinning canopy, which eventually death infected tree. The management containment this requires rapid reliable detection soil. current method for soil utilizes bioassay involving lupin...
Rhizoctonia is a major pathogen of potato causing substantial yield losses worldwide. Control diseases based predominantly on the application fungicides. However, little known about fungicide response variability different anastomosis groups associated with in South Africa. A total 131 isolates were obtained from growing regions Africa 2012 to 2014 and evaluated for sensitivity fungicides vitro vivo. The comprised six chemical formulations one bio-fungicide representing seven Fungicide...
On-site diagnosis of plant diseases can be a useful tool for growers timely decisions enabling the earlier implementation disease management strategies that reduce impact disease. Presently in many diagnostic laboratories, polymerase chain reaction (PCR), particularly real-time PCR, is considered most sensitive and accurate method pathogen detection. However, laboratory-based PCRs typically require expensive laboratory equipment skilled personnel. In this study, soil-borne pathogens potato...
Rhizoctonia solani consists of 13 anastomosis groups (AGs) designated AG1 to 13. AG3-PT is considered the predominant AG in potatoes (4) and associated with quantitative qualitative yield losses. Qualitative losses are typically tuber blemish disease, black scurf. However, atypical blemishes such as elephant hide consisting corky lesions on surface (2) have also been attributed Rhizoctonia. Such not specific Rhizoctonia, making direct-cause effect estimates difficult (1). Koch's postulates...
A survey of the relative incidence anastomosis groups (AGs) Rhizoctonia spp. associated with potato disease was conducted in Idaho, leading producing state U.S.A. In total, 169 isolates solani and seven binucleate (BNR) were recovered from diseased plants. The AG each isolate determined through real-time PCR assays for 3-PT phylogenetic analysis internal transcribed spacer region ribosomal DNA. predominant AG, accounting 85% recovered, followed by 2-1 (5.7%) 4 HG-II (4.5%). Two different...
Because of the widespread use engineered nanoparticles (ENPs) in consumer and industrial products, it is inevitable that these materials will enter environment. It often stated uptake ENPs into organisms environment related to particle size surface functionality. To test this assumption, present study investigated depuration gold nanoparticle (Au NPs) coated with either citrate (Au-citrate NPs), mercaptoundecanoic acid (Au-MUDA amino polyethylene glycol (PEG) thiol (Au-NH2 or PEG (Au-PEG NP)...
Soilborne Rhizoctonia, Microdochium, and Fusarium species are major causal agents of seedling stem-base diseases wheat. Currently, seed treatments considered the most effective solution for their control. Rhizoctonia solani anastomosis groups (AGs) 2-1 5, R. cerealis, spp., were used in series field experiments to determine capability cause soilborne disease quantify comparative losses establishment yield The effectiveness response treatment formulated with 10 g sedaxane 5 fludioxonil 100...
Early detection of causal pathogens is important to prevent crop loss from diseases. However, some diseases, such as soilborne are difficult diagnose due the absence visible or characteristic symptoms. In present study, use Oxford Nanopore MinION sequencer a molecular diagnostic tool was assessed its long-read sequencing capabilities and portability. Nucleotide samples (DNA RNA) potato field soils were sequenced analyzed using locally curated pathogen database, followed by identification via...
Rhizoctonia solani causes economically important diseases on potatoes and sugarbeet throughout the world (2). R. is a species complex of 13 anastomosis groups (AGs) which AG3-PT most commonly associated with potato AG2-2 AG4 sugarbeet. However, several AGs, including AG4, have been recorded causing (2,3). In summer 2012, plants in fields Idaho were sampled for solani. Isolations attempted from symptomatic plants. DNA extracted resulting pure cultures was screened using real-time PCR assay...