A5009287997- Plant Reproductive Biology
- Plant Molecular Biology Research
- Microtubule and mitosis dynamics
- Photosynthetic Processes and Mechanisms
- Plant nutrient uptake and metabolism
- Plant tissue culture and regeneration
- Autophagy in Disease and Therapy
- Cell death mechanisms and regulation
- Plant responses to water stress
- Cellular Mechanics and Interactions
- Peroxisome Proliferator-Activated Receptors
- Legume Nitrogen Fixing Symbiosis
- Plant Stress Responses and Tolerance
- Plant-Microbe Interactions and Immunity
- Polysaccharides and Plant Cell Walls
- Nematode management and characterization studies
- Protist diversity and phylogeny
- Phagocytosis and Immune Regulation
- Plant Parasitism and Resistance
- Lipid metabolism and biosynthesis
- CRISPR and Genetic Engineering
- Microbial Metabolites in Food Biotechnology
- Plant Surface Properties and Treatments
- Weed Control and Herbicide Applications
- Calcium signaling and nucleotide metabolism
Washington State University
2015-2024
Durham University
2008-2021
Queen's University Belfast
2013-2017
Institute of Biological Chemistry, Academia Sinica
2014
Swedish University of Agricultural Sciences
2005
Universidad de Málaga
2005
Royal Holloway University of London
1998-2002
University College London
2001
University of London
2001
Medical Research Council
1998
Programmed cell death (PCD) is indispensable for eukaryotic development. In animals, PCD executed by the caspase family of cysteine proteases. Plants do not have close homologues caspases but possess a phylogenetically distant proteases named metacaspases. The cellular function metacaspases in unknown. Here we show that during plant embryogenesis, metacaspase mcII-Pa translocates from cytoplasm to nuclei terminally differentiated cells are destined elimination, where it colocalizes with...
ABSTRACT Bacillus subtilis spores are being used for oral bacteriotherapy and bacterioprophylaxis of gastrointestinal disorders in both humans animals. Since B. is an aerobic saprophyte, how may benefit the gut microbiota intriguing question, since other probiotics such as Lactobacillus spp. which colonize anerobes. As a first step understanding potential effects ingesting spores, we have characterized five commercial products. An extensive biochemical, physiological, phylogenetic analysis...
The 65-kD microtubule-associated protein (MAP65) family is a of plant microtubule-bundling proteins. Functional analysis complicated by the heterogeneity within this family: there are nine MAP65 genes in Arabidopsis thaliana, AtMAP65-1 to AtMAP65-9. To begin functional dissection proteins, we have concentrated on single isoform, AtMAP65-1, and examined its effect dynamics mammalian microtubules. We show that recombinant does not promote polymerization stabilize microtubules against...
The type II inner nuclear membrane protein emerin is a component of the LINC complex that connects lamina to actin cytoskeleton. In emerin-null or -deficient human dermal fibroblasts we find centrosome detached from nucleus. Moreover, following siRNA knockdown in wild-type fibroblasts, also becomes We show interacts with tubulin, and nocadozole-treated cells phenocopy characteristic emerin-null/deficient cells. significant fraction located at outer peripheral ER, where it directly...
Although animals eliminate apoptotic cells using macrophages, plants use cell corpses throughout development and disassemble in a cell-autonomous manner by vacuolar death. During death, lytic vacuoles gradually engulf digest the cytoplasmic content. On other hand, acute stress triggers an alternative necrosis, which is characterized mitochondrial dysfunction, early rupture of plasma membrane, disordered disassembly. How both types death are regulated remains obscure. In this paper, we show...
Abstract Tudor Staphylococcal Nuclease (TSN or Tudor-SN; also known as SND1) is an evolutionarily conserved protein involved in the transcriptional and posttranscriptional regulation of gene expression animals. Although TSN was found to be indispensable for normal plant development stress tolerance, molecular mechanisms underlying these functions remain elusive. Here, we show that Arabidopsis thaliana essential integrity function cytoplasmic messenger ribonucleoprotein (mRNP) complexes...
Abstract Typical plant membranes and storage lipids are comprised of five common fatty acids yet over 450 unusual accumulate in seed oils various species. Plant important human animal nutrients, while some such as hydroxylated (HFA) used the chemical industry (lubricants, paints, polymers, cosmetics, etc.). Most extracted from non-agronomic crops leading to high production costs. Attempts engineer HFA into unsuccessful due bottlenecks overlapping pathways oil membrane lipid synthesis where...
Pollen tube growth is dependent on a dynamic actin cytoskeleton, suggesting that actin-regulating proteins are involved. We have examined the regulation of lily pollen-specific actin-depolymerizing factor (ADF) LlADF1. Its binding and depolymerizing activity pH sensitive, inhibited by certain phosphoinositides, but not controlled phosphorylation. Compared with its F-actin properties, low in depolymerization assays has been used to explain why pollen ADF decorates grains. This incompatible...
Summary Maize actin‐depolymerizing factor, ZmADF, binds both G‐ and F‐actin enhances in vitro actin dynamics. Evidence from studies on vertebrate ADF/cofilin supports the view that this class of protein responds to intracellular extracellular signals causes reorganization. As a test determine whether such signal‐responsive pathways existed plants, study addressed ability maize ADF be phosphorylated likely effects phosphorylation its capacity modulate It is shown ADF3 (ZmADF3) can by...
Cell division depends on the fine control of both microtubule dynamics and organisation. The bundling protein MAP65 is a ;midzone MAP' essential for integrity anaphase spindle cell division. Arabidopsis thaliana MAP65-1 (AtMAP65-1) binds bundles microtubules by forming 25 nm cross-bridges. Moreover, as AtMAP65-1 in interphase, telophase but does not bind prophase or metaphase, its activity through cycle must be under tight control. Here we show that hyperphosphorylated during prometaphase...
Summary Cell and tissue patterning in plant embryo development is well documented. Moreover, it has recently been shown that successful embryogenesis reliant on programmed cell death (PCD). The cytoskeleton governs morphogenesis. However, surprisingly little known about the role of associated PCD. We have used gymnosperm, Picea abies , somatic model system to address this question. Formation apical–basal embryonic pattern P. proceeds through establishment three major types: meristematic...
The dynamic behaviour of the actin cytoskeleton in plants relies on coordinated action several classes actin-binding proteins (ABPs). These ABPs include plant-specific subfamilies actin-nucleating formin proteins. model plant species Arabidopsis thaliana has over 20 proteins, all which contain regions place GTPase-binding domain, homology (FH)3 and DAD DID motifs found many fungal animal formins. We have identified for first time a region membrane-integrated AtFH4 that mediates an...