A5103443131- Autophagy in Disease and Therapy
- Plant tissue culture and regeneration
- Plant nutrient uptake and metabolism
- Plant Molecular Biology Research
- Protease and Inhibitor Mechanisms
- Peptidase Inhibition and Analysis
- Insect Resistance and Genetics
- Photosynthetic Processes and Mechanisms
- Cell death mechanisms and regulation
- Mosquito-borne diseases and control
- Plant responses to water stress
- Polyamine Metabolism and Applications
- Plant Stress Responses and Tolerance
- Enzyme Production and Characterization
- CRISPR and Genetic Engineering
- Plant Reproductive Biology
- Enzyme Structure and Function
- Studies on Chitinases and Chitosanases
- Ubiquitin and proteasome pathways
- Potato Plant Research
- Cell Adhesion Molecules Research
- Malaria Research and Control
- Soybean genetics and cultivation
- Adenosine and Purinergic Signaling
- Edible Oils Quality and Analysis
Universidad de Málaga
2005-2025
Duke University
2023-2024
Central University
2024
Instituto de Investigación Sanitaria del Principado de Asturias
2017-2023
Universidad de Murcia
2020
Tufts Medical Center
2020
Tufts University
2020
Universidad de Oviedo
2005-2013
Corporación Universitaria del Meta
2013
Medicina
2013
Programmed cell death (PCD) is indispensable for eukaryotic development. In animals, PCD executed by the caspase family of cysteine proteases. Plants do not have close homologues caspases but possess a phylogenetically distant proteases named metacaspases. The cellular function metacaspases in unknown. Here we show that during plant embryogenesis, metacaspase mcII-Pa translocates from cytoplasm to nuclei terminally differentiated cells are destined elimination, where it colocalizes with...
Autophagy is a major catabolic process whereby autophagosomes deliver cytoplasmic content to the lytic compartment for recycling. Autophagosome formation requires two ubiquitin-like systems conjugating Atg12 with Atg5, and Atg8 lipid phosphatidylethanolamine (PE), respectively. Genetic suppression of these causes autophagy-deficient phenotypes reduced fitness longevity. We show that Atg5 E1-like enzyme, Atg7, are rate-limiting components Atg8–PE conjugation in Arabidopsis. Overexpression...
Although animals eliminate apoptotic cells using macrophages, plants use cell corpses throughout development and disassemble in a cell-autonomous manner by vacuolar death. During death, lytic vacuoles gradually engulf digest the cytoplasmic content. On other hand, acute stress triggers an alternative necrosis, which is characterized mitochondrial dysfunction, early rupture of plasma membrane, disordered disassembly. How both types death are regulated remains obscure. In this paper, we show...
Abstract In the last years, autophagy has been revealed as an essential pathway for multiple biological processes and physiological functions. As a catabolic route, regulation by nutrient availability evolutionarily conserved from yeast to mammals. On one hand, induction starvation is associated with significant loss in body weight mice. Here, we demonstrate that both genetic pharmacological inhibition of process compromise induced starvation. Moreover, autophagic potential also impacts on...
The identification of inflammatory bowel disease (IBD) susceptibility genes by genome-wide association has linked this pathology to autophagy, a lysosomal degradation pathway that is crucial for cell and tissue homeostasis. Here, we describe autophagy-related 4B, cysteine peptidase/autophagin-1 (ATG4B) as an essential protein in the control response during experimental colitis. In pathological condition, ATG4B levels increase parallel with induction autophagy. Moreover, expression...
Autophagy is a housekeeping catabolic process crucial for maintaining cell, tissue and organism functions. Through the years, study of animal models with tissue-specific inactivation autophagy essential genes has allowed us to understand its protective roles in context multiple human diseases, including cancer neurodegeneration. However, due nature autophagy, effects systemic inhibition mammals have not been explored detail. Here, we report generation ATG4A-only mice, simultaneously...
Somatic embryogenesis of Norway spruce (Picea abies L.) is a versatile model system to study molecular mechanisms regulating embryo development because it proceeds through defined developmental stages corresponding specific culture treatments. Normal embryonic involves early differentiation proembryogenic masses (PEMs) into somatic embryos, followed by and late embryogeny leading the formation mature cotyledonary embryos. In some cell lines there arrest at PEM−somatic transition. To learn...
Caloric restriction (CR) extends lifespan in various heterotrophic organisms ranging from yeasts to mammals, but whether a similar phenomenon occurs plants remains unknown. Plants are autotrophs and use their photosynthetic machinery convert light energy into the chemical of glucose other organic compounds. As rate photosynthesis is proportional level photosynthetically active radiation, CR can be modeled by lowering intensity. Here, we report that low intensity Arabidopsis through...
We define Dry Rivers as those whose usual habitat in space and time are dry channels where surface water may interrupt conditions for hours or a few days, primarily after heavy rainfall events that variable usually lead to flash floods, disconnected from groundwater thereby unable harbor aquatic life. Conceptually, would represent the extreme of hydrological continuum increased flow interruption typically characterizes non-perennial rivers, thus being preceded by intermittent ephemeral...
Summary In this paper, we report the identification of genes from pine ( PpAAT ), Arabidopsis AtAAT ) and rice OsAAT encoding a novel class aspartate aminotransferase (AAT, EC 2.6.1.1) in plants. The enzyme is unrelated to other eukaryotic AATs plants animals but similar bacterial enzymes. Phylogenetic analysis indicates that prokaryotic‐type AAT closely related cyanobacterial enzymes, suggesting it might have an endosymbiotic origin. Interestingly, most essential residues involved...
Abstract Ongoing habitat loss and species extinctions require managers to implement quantify the effectiveness of conservation actions for protecting biodiversity. Fencing, when done properly, is an important management tool in landscapes where wildlife domestic animals co‐occur, potentially enhancing use through selective exclusion species. For instance, fencing forest patches Neotropics expected reduce degradation understory vegetation by cattle, releasing these resources native community...
Ammonium assimilation during the initial stages of Scots pine growth involves two cytosolic glutamine synthetase (GS, EC: 6.3.1.2) isoenzymes encoded by separate genes, GS1a and GS1b. was most exclusively expressed in photosynthetic tissues seedling whereas GS1b ubiquitously showing higher levels non-photosynthetic such as root hypocotyl. Temporal expression analysis has shown that when germination starts is predominant form embryo, however, its relative abundance tissue decreased...
NADP+-isocitrate dehydrogenase (NADP+-IDH; EC 1.1.1.42) is involved in the supply of 2-oxoglutarate for ammonia assimilation and glutamate synthesis higher plants through glutamine synthetase/glutamate synthase (GS/GOGAT) cycle. Only one NADP+-IDH form cytosolic localization was detected green cotyledons pine (Pinus spp.) seedlings. The enzyme purified exhibited molecular kinetic properties similar to those described from angiosperm, with a catalytic efficiency (10(5) M-1 s-1) than deduced...
• Here, embryo-specific patterns of glutamine synthetase (GS) genes were studied for the first time using pine somatic and zygotic embryogenesis as model systems. GS1a expression was absent in embryos whereas it detected cotyledons at late developmental stages along with transcripts photosynthesis arginase. These findings suggest that germination initiated maturing embryos. GS1b found mainly procambial cells both Expression before differentiation mature vascular elements indicated this gene...