A5058216144- Plant tissue culture and regeneration
- Plant Reproductive Biology
- Seed Germination and Physiology
- Bioenergy crop production and management
- Forest ecology and management
- Forest Ecology and Biodiversity Studies
- Plant biochemistry and biosynthesis
- Horticultural and Viticultural Research
- Plant Gene Expression Analysis
- Plant Pathogens and Fungal Diseases
- Forest Biomass Utilization and Management
- Plant Molecular Biology Research
- Genetic diversity and population structure
- Plant Taxonomy and Phylogenetics
- Plant and Fungal Interactions Research
- Plant Diversity and Evolution
- Nuts composition and effects
- Forest Insect Ecology and Management
- Mycorrhizal Fungi and Plant Interactions
- Photosynthetic Processes and Mechanisms
- Genetic and phenotypic traits in livestock
- Plant and animal studies
- Enzyme-mediated dye degradation
- Glycosylation and Glycoproteins Research
- Chromosomal and Genetic Variations
Institut Technologique Forêt Cellulose Bois-Construction Ameublement
2011-2022
Centre d'Études Scientifiques et Techniques d'Aquitaine
2013-2020
Forêt Méditerranéenne
2013
Laboratoire de Biotechnologie de l'Environnement
2008-2010
Laboratoire du Futur
2000-2001
Gembloux Agro-Bio Tech
1994-1997
Summary Maritime pine ( P inus pinaster A it.) is a widely distributed conifer species in S outhwestern E urope and one of the most advanced models for research. In current work, comprehensive characterization maritime transcriptome was performed using combination two different next‐generation sequencing platforms, 454 I llumina. De novo assembly provided catalogue 26 020 unique transcripts trees collection 9641 full‐length c DNA s. Quality validated by RT ‐ PCR amplification selected...
Maritime pine somatic embryos (SEs) require a reduction in water availability (high gellan gum concentration the maturation medium) to reach cotyledonary stage. This key switch, reported specifically for species, is not yet well understood. To facilitate use of embryogenesis mass propagation conifers, we need better understanding embryo development. Comparison both transcriptome (Illumina RNA sequencing) and proteome [two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis...
Abstract An efficient procedure has been developed for DNA extraction from cotton by modifying the original CTAB method. The major improvement concerns use of activated charcoal to bind resinous and coloured compounds which copurify with DNA. efficiency amplification RAPD was used as a criterion evaluate action charcoal. Twenty‐five random decamers Operon Technologies were compare samples extracted in presence or absence results terms suggest that enhances amplification. This technique...
Cryopreservation of a core collection 444 elm (Ulmus spp.) clones in liquid nitrogen was carried out by two laboratories participating European project conservation genetic resources. Plant material, collected nine countries, represented large sample the diversity within three species and their hybrids. The cryopreservation technique used both involved stepwise freezing cryotubes containing dormant buds. Comparisons with fresh buds showed that treatment had no negative effect on viability...
Background There is considerable interest in the high-throughput discovery and genotyping of single nucleotide polymorphisms (SNPs) to accelerate genetic mapping enable association studies. This study provides an assessment EST-derived resequencing-derived SNP quality maritime pine (Pinus pinaster Ait.), a conifer characterized by huge genome size (∼23.8 Gb/C). Methodology/Principal Findings A 384-SNPs GoldenGate array was built from i/ 184 SNPs originally detected set 40 re-sequenced...
Glutathione transferases (GSTs) form a superfamily of multifunctional proteins with essential roles in cellular detoxification processes and endogenous metabolism. The distribution fungal-specific class A GSTs was investigated saprotrophic fungi revealing recent diversification within this class. Biochemical characterization eight GSTFuA isoforms from Phanerochaete chrysosporium Coprinus cinereus demonstrated functional diversity fungi. three-dimensional structures three P. feature...
Although eucalypts are the most planted hardwood trees worldwide, majority of them frost sensitive. The recent creation frost-tolerant hybrids such as Eucalyptus gundal plants (E. gunnii × E. dalrympleana hybrids), now enables development industrial plantations in northern countries. Our objective was to evaluate impact cold on wood structure and composition these hybrids, biosynthetic regulatory processes controlling their secondary cell-wall (SCW) formation. We used an integrated approach...
Vegetative propagation through somatic embryogenesis is critical in conifer biotechnology towards multivarietal forestry that uses elite varieties to cope with environmental and socio-economic issues. An important still sub-optimal process during vitro maturation of embryos (SE) the biosynthesis deposition storage proteins, which are rich amino acids high nitrogen (N) content, such as arginine. Mobilization these N-rich proteins essential for germination production vigorous seedlings....
Annotation of the Eucalyptus grandis genome showed a large amplification dehydration-responsive element binding 1/C-repeat factor (DREB1/CBF) group without recent DREB2 gene duplication compared with other plant species. The present annotation CBF and genes from draft gunnii sequence reveals at least one additional copy in E. grandis, suggesting that this is still evolving, unlike group. This study aims to investigate redundancy/neo- or sub-functionalization duplicates relative involvement...
• Here, embryo-specific patterns of glutamine synthetase (GS) genes were studied for the first time using pine somatic and zygotic embryogenesis as model systems. GS1a expression was absent in embryos whereas it detected cotyledons at late developmental stages along with transcripts photosynthesis arginase. These findings suggest that germination initiated maturing embryos. GS1b found mainly procambial cells both Expression before differentiation mature vascular elements indicated this gene...