A5059760020- Plant Gene Expression Analysis
- Plant Physiology and Cultivation Studies
- Plant Molecular Biology Research
- Plant biochemistry and biosynthesis
- Plant Stress Responses and Tolerance
- Plant Water Relations and Carbon Dynamics
- Postharvest Quality and Shelf Life Management
- Plant-Microbe Interactions and Immunity
- Plant Reproductive Biology
- Plant nutrient uptake and metabolism
- Potato Plant Research
- Plant tissue culture and regeneration
- Lignin and Wood Chemistry
- Metabolomics and Mass Spectrometry Studies
- Spectroscopy and Chemometric Analyses
- Photosynthetic Processes and Mechanisms
- Polysaccharides and Plant Cell Walls
- Forest ecology and management
- Horticultural and Viticultural Research
- Growth and nutrition in plants
- Seedling growth and survival studies
- CRISPR and Genetic Engineering
- Insect and Arachnid Ecology and Behavior
- Bioenergy crop production and management
- Agricultural Practices and Plant Genetics
Laboratoire de Recherche en Sciences Végétales
2015-2024
Université de Toulouse
2015-2024
Centre National de la Recherche Scientifique
2015-2024
Université Toulouse III - Paul Sabatier
2015-2024
Laboratoire des Interactions Plantes Micro-Organismes
2024
London Rebuilding Society
2023
Biologie du Fruit et Pathologie
2007-2021
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2021
Université de Bordeaux
2009-2012
Biopolymères Interactions Assemblages
2009-2012
Variations in early fruit development and composition may have major impacts on the taste overall quality of ripe tomato (Solanum lycopersicum) fruit. To get insights into networks involved these coordinated processes to identify key regulatory genes, we explored transcriptional metabolic changes expanding tissues using multivariate analysis gene-metabolite correlation networks. this end, demonstrated took advantage existence clear structural compositional differences between mesocarp...
The plant cuticle consists of cutin, a polyester glycerol, hydroxyl, and epoxy fatty acids, covered filled by waxes. While the biosynthesis cutin building blocks is well documented, mechanisms underlining their extracellular deposition remain unknown. Among proteins extracted from dewaxed tomato (Solanum lycopersicum) peels, we identified GDSL1, member GDSL esterase/acylhydrolase family proteins. GDSL1 strongly expressed in epidermis growing fruit. In GDSL1-silenced lines, observed...
Abstract l-Galactono-1,4-lactone dehydrogenase (EC 1.3.2.3) catalyzes the last step in main pathway of vitamin C (l-ascorbic acid) biosynthesis higher plants. In this study, we first characterized spatial and temporal expression SlGalLDH several organs tomato (Solanum lycopersicum) plants parallel with ascorbate content. P35S:SlgalldhRNAi silenced transgenic lines were then generated using an RNAi strategy to evaluate effect any resulting modification pool on plant fruit development. all...
The PIN-FORMED (PIN) auxin efflux transport protein family has been well characterized in the model plant Arabidopsis thaliana, where these proteins are crucial for regulation of various aspects development. Recent evidence indicates that PIN may play a role fruit set and early development tomato (Solanum lycopersicum), but functional analyses PIN-silenced plants failed to corroborate this hypothesis. Here it is demonstrated silencing specifically SlPIN4 gene, which predominantly expressed...
Eucalypts are the most planted hardwoods worldwide. The availability of Eucalyptus grandis genome highlighted many genes awaiting functional characterization, lagging behind because lack efficient genetic transformation protocols. In order to efficiently generate knock-out mutants study function eucalypts genes, we implemented powerful CRISPR/Cas9 gene editing technology with hairy roots system. As proofs-of-concept, targeted two wood-related genes: Cinnamoyl-CoA Reductase1 (CCR1), a key...
Lignin is a major lock for lignocellulose valorization in biorefineries, prompting need to find new ligninolytic systems. Termites are efficient degraders, and large part of this ability comes from its anaerobic gut microbiome. However, the potential termite microbiomes degrade lignin under conditions has yet be elucidated. By applying wet chemistry, multidimensional NMR spectroscopy, quantitative 13C-IS py-GC-MS, we determined chemical structural characteristics wheat straw digested by...
Changes in photoassimilate partitioning between source and sink organs significantly affect fruit development size. In this study, a comparison was made of tomato plants (Solanum lycopersicum L.) grown under low load (one per truss, L1 plants) standard (five fruits L5 plants), at morphological, biochemical, molecular levels. Fruit reduction resulted increased availability the plant growth rates all analysed (root, stem, leaf, flower, fruit). Larger flower size were correlated with higher...
Background The AP2/ERF family includes a large number of developmentally and physiologically important transcription factors sharing an AP2 DNA-binding domain. Among them DREB1/CBF DREB2 are known as master regulators respectively cold heat/osmotic stress responses. Experimental Approaches manual annotation from Eucalyptus grandis, Malus, Populus Vitis genomes allowed complete phylogenetic study for comparing the structure this in woody species model Arabidopsis thaliana. Expression profiles...
Summary Wood production in fast‐growing Eucalyptus grandis trees is highly dependent on both potassium (K) fertilization and water availability but the molecular processes underlying wood formation response to combined effects of these two limiting factors remain unknown. E. were submitted four combinations K‐fertilization supply. Weighted gene co‐expression network analysis MixOmics‐based co‐regulation networks used integrate xylem transcriptome, metabolome complex traits. Functional...
Although eucalypts are the most planted hardwood trees worldwide, majority of them frost sensitive. The recent creation frost-tolerant hybrids such as Eucalyptus gundal plants (E. gunnii × E. dalrympleana hybrids), now enables development industrial plantations in northern countries. Our objective was to evaluate impact cold on wood structure and composition these hybrids, biosynthetic regulatory processes controlling their secondary cell-wall (SCW) formation. We used an integrated approach...
Temperature elevation drastically affects plant defense responses to
Annotation of the Eucalyptus grandis genome showed a large amplification dehydration-responsive element binding 1/C-repeat factor (DREB1/CBF) group without recent DREB2 gene duplication compared with other plant species. The present annotation CBF and genes from draft gunnii sequence reveals at least one additional copy in E. grandis, suggesting that this is still evolving, unlike group. This study aims to investigate redundancy/neo- or sub-functionalization duplicates relative involvement...
Fruit is a complex organ containing seeds and several interconnected tissues with dedicated roles. However, most biochemical or molecular studies about fleshy fruit development concern the entire fruit, without seeds, pericarp only. We studied tomato (Solanum lycopersicum) at four stages of (12, 20, 35, 45 days post-anthesis). separated other tissues, exocarp, mesocarp, columella placenta locular tissue, analyzed them individually using proton NMR metabolomic profiling for quantification...
Comparative phylogenetic analyses of the R2R3-MYB transcription factor family revealed that five subgroups were preferentially found in woody species and totally absent from Brassicaceae monocots (Soler et al., 2015). Here, we analyzed one these (WPS-I) for which no gene had been yet characterized. Most Eucalyptus members WPS-I are expressed vascular cambium, secondary meristem responsible tree radial growth. We focused on EgMYB88, is most specifically highly tissues, showed it behaves as a...
While potassium fertilization increases growth yield in Brazilian eucalyptus plantations, it could also increase water requirements, making trees more vulnerable to drought. Sodium fertilization, which has been shown promote compared K-deficient trees, partially mitigate this adverse effect of potassium. However, little is known about the influence K and Na on tree metabolic response deficit. The aim present study was thus analyze transcriptome leaves sampled from Eucalyptus grandis...
Deciphering the mechanism of secondary cell wall/SCW formation in plants is key to understanding their development and molecular basis biomass recalcitrance. Although transcriptional regulation essential for SCW formation, little known about implication post-transcriptional mechanisms this process. Here we report that two bonafide RNA-binding proteins homologous animal translational regulator Musashi, MSIL2 MSIL4, function redundantly control Arabidopsis. MSIL2/4 interactomes are similar...
Eucalypts are the most planted trees worldwide, but of them frost sensitive. Overexpressing transcription factors for CRT-repeat binding (CBFs) in transgenic Eucalyptus confer cold resistance both leaves and stems. While wood plays crucial roles is affected by environmental cues, its potential role adaptation to stress has been neglected. Here, we addressed this question investigating changes occurring response overexpression two CBFs, taking advantage available lines. We performed...
Wood (secondary xylem) formation is regulated by auxin, which plays a pivotal role as an integrator of developmental and environmental cues. However, our current knowledge auxin-signaling during wood incomplete. Our previous genome-wide analysis Aux/IAAs in Eucalyptus grandis showed the presence non-canonical paralog member EgrIAA20 that preferentially expressed cambium. We analyzed its cellular localization using GFP fusion protein transcriptional activity transactivation assays,...
Deciphering the mechanism of secondary cell wall/SCW formation in plants is key to understanding their development and molecular basis biomass recalcitrance. Although transcriptional regulation essential for SCW formation, little known about implication post-transcriptional mechanisms this process. Here we report that two bonafide RNA-binding proteins homologous animal translational regulator Musashi, MSIL2 MSIL4, function redundantly control Arabidopsis . MSIL2/4 interactomes are similar...
Abstract While being the most planted tree worldwide, Eucalyptus species are sensitive to frost. Long-term exposure cold temperatures, among other abiotic cues, triggers modification of secondary xylem differentiation in , but molecular mechanisms behind it remain unknown. Overexpression key players signalling pathway, CRT-repeat binding factors (CBF), not only causes expected increase freezing tolerance also remodels wood a similar trend acclimation, making good candidate for functional...