A5087693490- Plant Pathogens and Fungal Diseases
- Plant Gene Expression Analysis
- Plant biochemistry and biosynthesis
- Plant-Microbe Interactions and Immunity
- Genomics and Phylogenetic Studies
- Plant Disease Resistance and Genetics
- Genetic Mapping and Diversity in Plants and Animals
- Forest Insect Ecology and Management
- Biofuel production and bioconversion
- Malaria Research and Control
- Genomic variations and chromosomal abnormalities
- Plant Molecular Biology Research
- Forest ecology and management
- Nematode management and characterization studies
- Yeasts and Rust Fungi Studies
- Plant and animal studies
- Plant pathogens and resistance mechanisms
- Lignin and Wood Chemistry
- Prenatal Screening and Diagnostics
- Insect Pest Control Strategies
- Plant Pathogenic Bacteria Studies
- Plant Physiology and Cultivation Studies
- Genomics and Chromatin Dynamics
- Molecular Biology Techniques and Applications
- Tree-ring climate responses
University of Pretoria
2016-2024
Forestry and Agricultural Biotechnology Institute
2022
Agricultural Biotechnology Institute
2022
Eucalyptus grandis is a commercially important hardwood species and known to be susceptible number of pests pathogens. Determining mechanisms defense therefore research priority. The published genome for E. has aided the identification one class resistance (R) genes that incorporate nucleotide binding sites leucine-rich repeat domains (NBS-LRR). Using an iterative search process we identified NBS-LRR gene models within genome. We characterized their genomic arrangement. expression patterns...
The Democratic Republic of the Congo (DRC) is characterized as a holoendemic malaria area with main vectors being Anopheles funestus and members gambiae complex. Due to political instability socio-economic challenges in region, knowledge insecticide resistance status mechanisms these limited. Mosquitoes were collected from mining site north-eastern part country and, following identification, subjected extensive testing for target-site biochemical basis resistance. Quantitative real-time PCR...
Summary We used a systems genetics approach to elucidate the molecular mechanisms of responses maize grey leaf spot ( GLS ) disease caused by Cercospora zeina , threat production globally. Expression analysis earleaf samples in subtropical recombinant inbred line population CML 444 × SC Malawi) subjected field C. infection allowed detection 20 206 expression quantitative trait loci eQTL s). Four trans ‐ hotspots coincided with QTL s mapped same experiment. Co‐expression network identified...
Significance Carbon fixation and accumulation as lignocellulosic biomass is of global ecological industrial importance most significantly occurs in the form wood development trees. Traits are highly complex and, aside from environmental factors, affected by many pathways thousands genes. We have applied a network-based data integration method for systems genetics analysis genes, processes, underlying bioenergy-related traits using segregating Eucalyptus hybrid tree populations. could link...
Eucalyptus grandis (W. Hill ex Maiden) is an Australian Myrtaceae tree grown for timber in many parts of the world and which annotated genome sequence available. Known to be susceptible a number pests diseases, E. useful study organism investigating defense responses woody plants. Chitinases are widespread plants cleave glycosidic bonds chitin, major structural component fungal cell walls arthropod exoskeletons. They encoded by important class genes known up-regulated response pathogens. The...
Leptocybe invasa is an insect pest causing gall formation on oviposited shoot tips and leaves of Eucalyptus trees leading to leaf deformation, stunting, death in severe cases. We previously observed different constitutive induced terpenes, plant specialized metabolites that may act as attractants or repellents insects, a resistant susceptible clone challenged with L. invasa. tested the hypothesis specific terpenes are associated resistance grandis half-sib population. Insect damage was...
We performed gene and genome targeted SNP discovery towards the development of a genome-wide, multispecies genotyping array for tropical pines. Pooled RNA-seq data from shoots seedlings five pine species was used to identify transcript-based SNPs resulting in 1.3 million candidate Affymetrix probe sets. In addition, we custom 40 K set perform capture-seq pooled DNA 81 provenances representing natural ranges six Mexico Central America 563 Altogether, 300 (72%) 120 (28%) derived sets were...
Summary Acetyl‐ and methylglucuronic acid decorations of xylan, the dominant hemicellulose in secondary cell walls (SCWs) woody dicots, affect its interaction with cellulose lignin to determine SCW structure extractability. Genes pathways involved these modifications may be targets for genetic engineering; however, little is known about regulation xylan plants. To address this, we assessed gene expression variation associated modification developing xylem Eucalyptus grandis × urophylla...
Maize underpins food security in South Africa. An annual production of more than 10 million tons is a combination the output large-scale commercial farms plus an estimated 250 000 ha cultivated by smallholder farmers. leaves are rich source nutrients for fungal pathogens. Farmers must limit leaf blighting fungi to prevent sugars captured photosynthesis being ‘stolen’ instead filling grain. This study aimed fill knowledge gap on prevalence and impact foliar diseases local maize fields. A...
Many pests and pathogens threaten Eucalyptus plantations. The study of defense responses in this economically important wood fiber crop enables the discovery novel pathways genes, which may be adopted to improve resistance. Various functional genomics experiments have been conducted Eucalyptus-biotic stress interactions following availability grandis genome, however, comparisons between these studies were limited largely due a lack comparative tools. To end, we developed eCALIBRATOR...
To improve our understanding of genetic mechanisms underlying complex traits in plants, a comprehensive analysis gene variants is required. Eucalyptus an important forest plantation genus that highly outbred. Trait dissection and molecular breeding eucalypts currently relies on biallelic single-nucleotide polymorphism (SNP) markers. These markers fail to capture the large amount haplotype diversity these species, thus multi-allelic are We aimed develop gene-based mining panel for species....
Plantation forests were introduced into South Africa to satisfy the regional demand for wood-based products, which are largely found in Mpumalanga province. To better understand impacts of climate on dominant height growth commercially important plantation species, enumeration data collected from 2012 2023 was scrutinised, representing 34740 plots 2082 compartments. Statistically significant multiple linear regression models developed predict heights Pinus elliottii, taeda, patula, and...
Affordable high-throughput DNA and RNA sequencing technologies are allowing genomic analysis of plant animal populations as a result empowering new systems genetics approaches to study complex traits. The availability intuitive tools browse analyze the resulting large-scale genetic datasets remain significant challenge. Furthermore, these integrative genomics require innovative methods dissect flow interconnectedness biological information underlying trait variation. Plant Genome Integrative...
Summary To improve our understanding of genetic mechanisms underlying complex traits in plants, a comprehensive analysis gene variants is required. Eucalyptus an important forest plantation genus that highly outbred. Trait dissection and molecular breeding eucalypts currently relies on biallelic SNP markers. These markers fail to capture the large amount haplotype diversity these species thus multi-allelic are We aimed develop gene-based mining panel for species. generated 17 999...
Abstract A multi-tiered transcriptional network regulates xylem differentiation and secondary cell wall (SCW) formation in plants, with evidence of both conserved lineage-specific SCW architecture. We aimed to elucidate the roles selected R2R3-MYB transcription factors (TFs) linked Eucalyptus wood by identifying genome-wide TF binding sites direct target genes through an improved DAP-seq protocol combined machine learning for gene assignment (DAP-seq-ML). applied this five TFs including a...
Standard chromosome nomenclatureContributors to this journal are urged familiarize themselves with the nomenclature systems that have been developed for many animal species.Such applicable not only normal karyotypes but fre quently those of established cell lines, somatic hybrids, and tumor cells (see Mu.U R, page 190 issue).The mitotic meiotic chromosomes man great apes, as well acquired chromo some aberrations, should follow recom mendations ISCN (1978).The International Standing Committee...