A5103251945- Plant Gene Expression Analysis
- Plant biochemistry and biosynthesis
- Plant Molecular Biology Research
- Plant-Microbe Interactions and Immunity
- Lignin and Wood Chemistry
- Plant Pathogens and Fungal Diseases
- Plant Stress Responses and Tolerance
- Photosynthetic Processes and Mechanisms
- Plant Parasitism and Resistance
- Plant Water Relations and Carbon Dynamics
- RNA Research and Splicing
- Biochemical and biochemical processes
- Molecular Biology Techniques and Applications
- CRISPR and Genetic Engineering
- Plant Physiology and Cultivation Studies
- Horticultural and Viticultural Research
- Genetic and Environmental Crop Studies
- Lipid metabolism and biosynthesis
- Sunflower and Safflower Cultivation
- Plant Disease Resistance and Genetics
- Plant Pathogenic Bacteria Studies
- Seed Germination and Physiology
- Insect symbiosis and bacterial influences
- Biofuel production and bioconversion
- Plant tissue culture and regeneration
Centre National de la Recherche Scientifique
2009-2024
Université Toulouse III - Paul Sabatier
2010-2024
Laboratoire de Recherche en Sciences Végétales
2009-2024
Université de Toulouse
2008-2024
Institut National Polytechnique de Toulouse
2005
• The eucalyptus R2R3 transcription factor, EgMYB1 contains an active repressor motif in the regulatory domain of predicted protein. It is preferentially expressed differentiating xylem and capable repressing two key lignin genes vivo. In order to investigate planta role this putative transcriptional biosynthetic pathway, we overexpressed gene Arabidopsis poplar. Expression produced similar phenotypes both species, with stronger effects transgenic plants than Vascular development was altered...
Eucalyptus are of tremendous economic importance being the most planted hardwoods worldwide for pulp and paper, timber bioenergy. The recent release grandis genome sequence pointed out many new candidate genes potentially involved in secondary growth, wood formation or lineage-specific biosynthetic pathways. Their functional characterization is, however, hindered by tedious, time-consuming inefficient transformation systems available hitherto eucalypts. To overcome this limitation, we...
Interest in the genomics of Eucalyptus has skyrocketed thanks to recent sequencing genome grandis and a growing number large-scale transcriptomic studies. Quantitative reverse transcription–PCR (RT–PCR) is method choice for gene expression analysis can now also be used as high-throughput method. The selection appropriate internal controls becoming utmost importance ensure accurate results Eucalyptus. To this end, we selected 21 candidate reference genes microfluidic dynamic arrays assess...
Eucalypts are the most planted hardwoods worldwide. The availability of Eucalyptus grandis genome highlighted many genes awaiting functional characterization, lagging behind because lack efficient genetic transformation protocols. In order to efficiently generate knock-out mutants study function eucalypts genes, we implemented powerful CRISPR/Cas9 gene editing technology with hairy roots system. As proofs-of-concept, targeted two wood-related genes: Cinnamoyl-CoA Reductase1 (CCR1), a key...
Abstract Background Renowned for their fast growth, valuable wood properties and wide adaptability, Eucalyptus species are amongst the most planted hardwoods in world, yet they still at early stages of domestication because conventional breeding is slow costly. Thus, there huge potential marker-assisted programs to improve traits such as properties. To this end, sequencing, analysis annotation a large collection expressed sequences tags (ESTs) from genes involved formation would provide...
The cellulose binding elicitor lectin (CBEL) of the genus Phytophthora induces necrosis and immune responses in several plant species, including Arabidopsis thaliana. However, role CBEL-induced outcome interaction is still unclear. This study shows that some defence responses, but not necrosis, required receptor-like kinase BAK1, a general regulator basal immunity Arabidopsis, production reactive oxygen burst mediated by respiratory oxidases homologues (RBOH). Screening core collection 48...
Although eucalypts are the most planted hardwood trees worldwide, majority of them frost sensitive. The recent creation frost-tolerant hybrids such as Eucalyptus gundal plants (E. gunnii × E. dalrympleana hybrids), now enables development industrial plantations in northern countries. Our objective was to evaluate impact cold on wood structure and composition these hybrids, biosynthetic regulatory processes controlling their secondary cell-wall (SCW) formation. We used an integrated approach...
Essential oil from Gaultheria procumbens is mainly composed of methylsalicylate (MeSA) (>96%), a compound which can be metabolized in plant tissues to salicylic acid, phytohormone inducing immunity against microbial pathogens. The potential use G. essential as biocontrol agent was evaluated on the model Arabidopsis thaliana. Expression selection defense genes detected 1, 6, and 24 h after treatment (0.1 ml/L) using high-throughput qPCR-based microfluidic technology. Control treatments...
Annotation of the Eucalyptus grandis genome showed a large amplification dehydration-responsive element binding 1/C-repeat factor (DREB1/CBF) group without recent DREB2 gene duplication compared with other plant species. The present annotation CBF and genes from draft gunnii sequence reveals at least one additional copy in E. grandis, suggesting that this is still evolving, unlike group. This study aims to investigate redundancy/neo- or sub-functionalization duplicates relative involvement...
Comparative phylogenetic analyses of the R2R3-MYB transcription factor family revealed that five subgroups were preferentially found in woody species and totally absent from Brassicaceae monocots (Soler et al., 2015). Here, we analyzed one these (WPS-I) for which no gene had been yet characterized. Most Eucalyptus members WPS-I are expressed vascular cambium, secondary meristem responsible tree radial growth. We focused on EgMYB88, is most specifically highly tissues, showed it behaves as a...
Eucalypts are the most planted trees worldwide, but of them frost sensitive. Overexpressing transcription factors for CRT-repeat binding (CBFs) in transgenic Eucalyptus confer cold resistance both leaves and stems. While wood plays crucial roles is affected by environmental cues, its potential role adaptation to stress has been neglected. Here, we addressed this question investigating changes occurring response overexpression two CBFs, taking advantage available lines. We performed...
With the current global focus on bioenergy, forest plantations are increasingly becoming important sources for second generation biofuel, where whole plant lignocellulosic biomass is to be mobilized. The mainly composed of secondary walls (SW) possessing unique characteristics (biochemical composition and tridimensional association polymers), which govern intrinsic properties wood. They especially contain high amounts lignins, hydrophobic phenolic polymers constitute an obstacle optimal...
Abstract While being the most planted tree worldwide, Eucalyptus species are sensitive to frost. Long-term exposure cold temperatures, among other abiotic cues, triggers modification of secondary xylem differentiation in , but molecular mechanisms behind it remain unknown. Overexpression key players signalling pathway, CRT-repeat binding factors (CBF), not only causes expected increase freezing tolerance also remodels wood a similar trend acclimation, making good candidate for functional...
Abstract A multi-tiered transcriptional network regulates xylem differentiation and secondary cell wall (SCW) formation in plants, with evidence of both conserved lineage-specific SCW architecture. We aimed to elucidate the roles selected R2R3-MYB transcription factors (TFs) linked Eucalyptus wood by identifying genome-wide TF binding sites direct target genes through an improved DAP-seq protocol combined machine learning for gene assignment (DAP-seq-ML). applied this five TFs including a...