A5089080548- Gut microbiota and health
- Diet and metabolism studies
- Liver Disease Diagnosis and Treatment
- Machine Learning in Bioinformatics
- RNA and protein synthesis mechanisms
- Genomics and Phylogenetic Studies
- Clostridium difficile and Clostridium perfringens research
- Dietary Effects on Health
- Advanced Proteomics Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Biochemical Analysis and Sensing Techniques
- Salivary Gland Disorders and Functions
- Pediatric Hepatobiliary Diseases and Treatments
- Oral microbiology and periodontitis research
- Otolaryngology and Infectious Diseases
- Hepatitis B Virus Studies
- IL-33, ST2, and ILC Pathways
- Child Nutrition and Feeding Issues
- Colorectal Cancer Screening and Detection
- Gastrointestinal motility and disorders
- Infective Endocarditis Diagnosis and Management
- Immune cells in cancer
- Bacterial Identification and Susceptibility Testing
- Mass Spectrometry Techniques and Applications
- Bioinformatics and Genomic Networks
Physiogenex (France)
2024
University of Rome Tor Vergata
2023
GTP Technology
2021
Syngenta (Switzerland)
2009-2012
Université Toulouse III - Paul Sabatier
2009
Centre National de la Recherche Scientifique
2009
Laboratoire de Recherche en Sciences Végétales
2009
McGill University
2005
European Bioinformatics Institute
2002-2004
Wellcome Trust
2002-2004
Recent studies have revealed that the blood of healthy humans is not as sterile previously supposed. The objective this study was to provide a comprehensive description microbiome present in different fractions individuals.The conducted 30 donors French national collection center (Établissement Français du Sang). We set up 16S rDNA quantitative polymerase chain reaction assay well targeted metagenomics sequencing pipeline specifically designed analyze microbiome, which we used on whole...
The early detection of liver fibrosis among patients with nonalcoholic fatty disease (NAFLD) is an important clinical need. In view the suggested role played by bacterial translocation in and obesity, we sought to investigate relationship between blood microbiota (LF) European cohorts severe obesity. We carried out a cross-sectional study obese patients, well characterized respect severity NAFLD, cohort FLORINASH. This has been divided into discovery comprising 50 Spanish then validation 71...
The ProDom database is a comprehensive set of protein domain families automatically generated from the SWISS-PROT and TrEMBL sequence databases. An associated database, ProDom-CG, has been derived as restriction to completely sequenced genomes. construction method based on iterative PSI-BLAST searches multiple alignments are for each family. web server provides user with tools visualise alignments, phylogenetic trees architectures proteins, well BLAST-based analyse new sequences homologous...
InterPro is a new integrated documentation resource for protein families, domains and functional sites, developed initially as means of rationalising the complementary efforts PROSITE, PRINTS, Pfam ProDom database projects.Merged annotations from PROSITE form core. Each combined entry includes descriptions literature references, links are made back to relevant parent database(s), allowing users see at glance whether particular family or domain has associated patterns, profiles, fingerprints,...
In brain, mRNAs are transported from the cell body to processes, allowing for local protein translation at sites distant nucleus. Using subcellular fractionation, we isolated a fraction rat embryonic day 18 brains enriched structures that resemble amorphous collections of ribosomes. This was mRNA encoding beta-actin, an is in dendrites and axons developing neurons. Abundant components this fraction, determined by tandem mass spectrometry, include ribosomal proteins, RNA-binding...
Background Substantial progress in high-throughput metagenomic sequencing methodologies has enabled the characterisation of bacteria from various origins (for example gut and skin). However, recently-discovered bacterial microbiota present within animal internal tissues remained unexplored due to technical difficulties associated with these challenging samples. Results We have optimized a specific 16S rDNA-targeted metagenomics (16S metabarcoding) pipeline based on Illumina MiSeq technology...
The association between gut dysbiosis, high intestinal permeability, and endotoxemia-mediated inflammation is well established in CKD. However, changes the circulating microbiome patients with CKD have not been studied. In this pilot study, we compare blood profile healthy controls using 16S ribosomal DNA sequencing.Blood bacterial was studied buffy coat samples quantitatively by PCR qualitatively targeted metagenomic sequencing a molecular pipeline specifically optimized for cross-sectional...
The consortium members are situated at different research centres around the world.
To demonstrate that glycemia and insulin resistance are controlled by a mechanism involving the adaptive immune system gut microbiota crosstalk.We triggered with microbial extracts specifically from intestinal ileum contents of HFD-diabetic mice process immunization. 35 days later, immunized were fed HFD for up to two months in order challenge development metabolic features. The responses quantified. Eventually, adoptive transfer cells microbiota-immunized naïve was performed causality...
Abstract Some obese subjects overeat lipid-rich foods. The origin of this eating behavior is unknown. We have here tested the hypothesis that these could be characterized by an impaired fatty taste sensitivity linked to a change in gustatory papillae microbial and salivary environment. composition microbiota saliva surrounding circumvallate was analyzed combination with orosensory lipid detection threshold normal weight (NW) (O) adults. Microbial architecture similar what known feces, but...
Abstract Background Renowned for their fast growth, valuable wood properties and wide adaptability, Eucalyptus species are amongst the most planted hardwoods in world, yet they still at early stages of domestication because conventional breeding is slow costly. Thus, there huge potential marker-assisted programs to improve traits such as properties. To this end, sequencing, analysis annotation a large collection expressed sequences tags (ESTs) from genes involved formation would provide...
Background The role of bacteria on the onset cardiovascular disease has been suggested. Reciprocally, increased intestinal bacterial translocation and bloodstream infection are common comorbidities associated with heart failure myocardial infarction ( MI ). In this context, aim study was to analyze blood microbiome in patients shortly after acute infarction. Methods Results We carried out a case control comparing 103 at high risk but free coronary 99 who had an . analyzed both quantitatively...
Abstract Key Points A higher microbiome load, possibly originating from different body sites, may be playing a pathogenic role in IgA nephropathy. Several taxonomic differences between patients with nephropathy and healthy controls are observed blood stool. Striking the gut confirm that does not directly reflect microbiome. Background (IgAN) has been associated dysbiosis, intestinal membrane disruption, translocation of bacteria into blood. Our study aimed to understand association...
Abstract Background Gut microbiota is involved in the development of liver diseases such as fibrosis. We and others identified that selected sets gut bacterial DNA bacteria translocate to tissues, notably liver, establish a non-infectious tissue composed microbial low frequency live bacteria. However, precise set DNA, thereby corresponding taxa associated with early stages fibrosis need be identified. Furthermore, overcome impact different group size patient origins we adapted innovative...
The applications of InterPro span a range biologically important areas that includes automatic annotation protein sequences and genome analysis. In has been utilised to provide reliable characterisation sequences, identifying them as candidates for functional annotation. Rules based on the are stored operated through database called RuleBase. RuleBase is used main tool in sequence group at EBI apply unknown sequences. annotated distributed TrEMBL database. also provides means carry out...
ABSTRACT The gut-brain axis has emerged as a key player in the regulation of brain function and cognitive health. Gut microbiota dysbiosis been observed preclinical models Alzheimer’s disease patients. Manipulating composition gut enhances or delays neuropathology deficits mouse models. Accordingly, health status animal facility may strongly influence these outcomes. In present study, we longitudinally analyzed fecal amyloid pathology 5XFAD mice housed specific opportunistic pathogen-free...
(1) Background: In developed countries, the prevalence of apical periodontitis (AP) varies from 20% to 50% for reasons that could be associated with microbiota ecology. (2) Methods: We performed a clinical study in Odontology department Toulouse hospital France, sequence 16S rRNA gene AP and collect parameters 94 patients. Forty-four patients were characterized PAI (periapical index severity) score lower or equal 3, while others had superior scores (n = 50). (3) Results: The low diversity...
Ticks are major vectors of diseases affecting humans such as Lyme disease or domestic animals anaplasmosis. Cross-alteration the vertebrate host skin microbiome and tick may be essential during process feeding for mechanism pathogen transmission. However, it has been poorly investigated.We used mice bitten by field-collected ticks (nymphs adult ticks) in different experimental conditions to investigate, 16S rRNA gene metabarcoding, impact blood on both mouse microbiome. We also investigated...
Abstract Background Colostrum is the first milk for a newborn. Its high content in microbiota shaping compounds and its intake at time of gut seeding suggests colostrum may be critical establishment healthy microbiota. There also accumulating evidence on importance growth. Here, we aimed to investigate contribution colostrum, colostrum-induced growth promotion. Addressing this question highly significant because (1) globally, less than half newborns are fully fed (2) prevention...
Abstract Human immunodeficiency virus (HIV) infection impairs mucosal immunity and leads to bacterial translocation, fueling chronic inflammation disease progression. While this is well established, questions remain about the compositional profile of translocated bacteria, what extent it influenced by antiretroviral therapy (ART). Using 16S ribosomal DNA targeted sequencing shotgun proteomics, we showed that HIV increases translocation from gut blood. increased alpha diversity in blood,...