A5020720649- Cellular transport and secretion
- Photosynthetic Processes and Mechanisms
- Endoplasmic Reticulum Stress and Disease
- Plant Reproductive Biology
- Microtubule and mitosis dynamics
- Amino Acid Enzymes and Metabolism
- Polyamine Metabolism and Applications
- Plant-Microbe Interactions and Immunity
- Plant Molecular Biology Research
- Mitochondrial Function and Pathology
- Metabolism and Genetic Disorders
- Lipid Membrane Structure and Behavior
- Plant Stress Responses and Tolerance
- Glycosylation and Glycoproteins Research
- Cancer-related Molecular Pathways
- Peptidase Inhibition and Analysis
- Pancreatic function and diabetes
- Cannabis and Cannabinoid Research
- Protein Structure and Dynamics
- Calcium signaling and nucleotide metabolism
- Plant nutrient uptake and metabolism
- Digestive system and related health
- RNA modifications and cancer
- Plant tissue culture and regeneration
- Hemoglobin structure and function
Universitat de València
2012-2023
Instituto de Biomedicina de Valencia
1994-2023
National Center for Genetic Engineering and Biotechnology
2021
European Molecular Biology Laboratory
1993-2007
University of Geneva
2001
Instituto de Biología Molecular y Celular de Plantas
2000
Universitat Politècnica de València
2000
Consejo Superior de Investigaciones Científicas
2000
University of California, San Diego
1993
Instituto Valenciano de Investigaciones Agrarias
1988-1991
A cascade of events is triggered upon the addition growth factor to quiescent mammalian cells, which ultimately restarts proliferation by inducing transition from G0/G1 S-phase. We have studied cyclin D1, a putative G1 cyclin, in normal diploid human fibroblasts. Cyclin D1 accumulated and reached maximum level before S-phase serum cells. The protein was localized nucleus, it disappeared nucleus as cells proceeded into Microinjection anti-cyclin antibodies or antisense plasmid prevented...
Summary Abscisic acid ( ABA ) is a major phytohormone involved in important stress‐related and developmental plant processes. Recent phosphoproteomic analyses revealed large set of ‐triggered phosphoproteins as putative mitogen‐activated protein kinase MAPK targets, although the evidence for s signalling still scarce. Here, we identified reconstituted vivo complete ‐activated cascade, composed MAP 3Ks 3K17/18, 2K MKK 3 four C group MPK 1/2/7/14. In planta , show that activation 7 blocked...
Mitogen‐activated protein kinase (MAPK) cascades play an important role in mediating stress responses plants. In Arabidopsis , 20 MAPKs have been identified and classified into four major groups (A–D). Little is known about the of group C MAPKs. We studied activation subgroup C1 (AtMPK1/AtMPK2) response to mechanical injury. An increase their activity was detected wounding that blocked by cycloheximide. Jasmonic acid (JA) activated AtMPK1/AtMPK2 absence wounding. Wound JA‐induction AtMPK1/2...
Plant cells possess much of the molecular machinery necessary for receptor-mediated endocytosis (RME), but this process still awaits detailed characterization. In order to identify a reliable and well-characterized marker investigate RME in plant cells, we have expressed human transferrin receptor (hTfR) Arabidopsis protoplasts. We found that hTfR is mainly endosomal (Ara7- FM4-64-positive) compartments, also at plasma membrane, where it mediates binding internalization its natural ligand...
p24 proteins constitute a family of putative cargo receptors that traffic in the early secretory pathway. can be divided into four subfamilies (p23, p24, p25 and p26) by sequence homology. In contrast to mammals yeast, most plant contain their cytosolic C‐terminus both dilysine motif −3, −4 position diaromatic −7, −8 position. We have previously shown tail Arabidopsis has ability interact with ARF1 coatomer (through motif) COPII subunits motif). Here, we establish localization trafficking...
p24 proteins are a family of type I membrane localized to compartments the early secretory pathway and coat protein (COPI)- COPII-coated vesicles. They can be classified, by sequence homology, into four subfamilies, named p24α, p24β, p24γ, p24δ. In contrast animals fungi, plants contain only members p24β p24δ subfamilies. It has previously been shown that transiently expressed red fluorescent (RFP)-p24δ5 localizes endoplasmic reticulum (ER) as consequence highly efficient COPI-based...
The Cdc2 protein kinase requires cyclin binding for activity and also binds to a small protein, Suc1. Charged-to-alanine scanning mutagenesis of was used previously localize A- B- Suc1-binding sites (B. Ducommun, P. Brambilla, G. Draetta, Mol. Cell. Biol. 11:6177-6184, 1991). Those were mapped by building model based on the crystallographic coordinates catalytic subunit cyclic AMP-dependent (cAPK) (D. R. Knighton, J. Zheng, L. F. Ten Eyck, V. A. Ashford, N.-H. Xuong, S. Taylor, M. Sowadski,...
Summary The p24 proteins belong to a family of type I membrane which cycle between the endoplasmic reticulum ( ER ) and Golgi via coat protein (COPI) COPII vesicles. Current nomenclature classifies them into four subfamilies, although plant either p24β or p24δ subfamilies. Here, we show that Arabidopsis p24δ5/δ9 HDEL ligands shift steady‐state distribution K/ receptor ERD 2 from . We also interact directly with 2. This interaction requires dynamics GOLD domain in p24δ5 is much higher at...
The p24 family of proteins (also known as the TMED family) are key regulators protein trafficking along secretory pathway, but very little is about their functions in plants. A quadruple loss-of-function mutant affecting genes from δ-1 subclass p24δ subfamily (p24δ3δ4δ5δ6) showed alterations Golgi, suggesting that these play a role organization compartments early pathway Arabidopsis Loss p24δ-1 also induced accumulation K/HDEL receptor ERD2a (ER lumen protein-retaining A) at Golgi and...
In contrast with the wealth of recent reports about function μ-adaptins and clathrin adaptor protein (AP) complexes, there is very little information motifs that determine sorting membrane proteins within clathrin-coated vesicles in plants. Here, we investigated putative signals large cytosolic loop Arabidopsis (Arabidopsis thaliana) PIN-FORMED1 (PIN1) auxin transporter, which are involved binding thus PIN1 trafficking localization. We found Phe-165 Tyr-280, Tyr-328, Tyr-394 different vitro....
COP (coat protein) I-coated vesicles mediate intra-Golgi transport and retrograde from the Golgi to endoplasmic reticulum. These form through action of small GTPase ADP-ribosylation factor 1 (ARF1) COPI heptameric protein complex (coatomer), which consists seven subunits (α-, β-, β′-, γ-, δ-, ε- ζ-COP). In contrast mammals yeast, several isoforms for coatomer subunits, with exception γ δ, have been identified in Arabidopsis. To understand role proteins plant biology, we characterized a...
The early secretory pathway involves bidirectional transport between the endoplasmic reticulum (ER) and Golgi apparatus is mediated by coat protein complex I (COPI)-coated II (COPII)-coated vesicles. COPII vesicles are involved in ER to meanwhile COPI mediate intra-Golgi retrograde from ER. key component of coatomer complex, that composed seven subunits (α/β/β'/γ/δ/ε/ζ). In Arabidopsis two genes coding for β-COP subunit have been identified, which result recent tandem duplication. Here we...
p24 proteins are a family of type-I membrane that cycle between the endoplasmic reticulum (ER) and Golgi apparatus via Coat Protein I (COPI)- COPII-coated vesicles. These have been proposed to function as cargo receptors, but identity putative cargos in plants is still elusive. We previously generated an Arabidopsis (Arabidopsis thaliana) quadruple loss-of-function mutant affecting genes from δ-1 subclass delta subfamily (p24δ3δ4δ5δ6 mutant). This also had reduced protein levels other was...
Abstract Glycosylphosphatidylinositol (GPI)-anchored proteins (GPI-APs) play an important role in a variety of plant biological processes including growth, stress response, morphogenesis, signaling, and cell wall biosynthesis. The GPI anchor contains lipid-linked glycan backbone that is synthesized the endoplasmic reticulum (ER) where it subsequently transferred to C-terminus containing signal peptide by transamidase. Once attached protein, lipid moieties are remodeled. In mammals yeast,...
p24 proteins are a family of type I membrane localized to compartments the early secretory pathway and coat protein (COPI)- COPII-coated vesicles. They can be classified, by sequence homology, into four subfamilies, named p24α, p24β, p24γ, p24δ. In contrast animals fungi, plants contain only members p24β p24δ latter probably including two different subclasses. It has previously been shown that transiently expressed red fluorescent (RFP)–p24δ5 (p24δ1 subclass) localizes endoplasmic reticulum...
The Cdc2 protein kinase requires cyclin binding for activity and also binds to a small protein, Suc1. Charged-to-alanine scanning mutagenesis of was used previously localize A- B- Suc1-binding sites (B. Ducommun, P. Brambilla, G. Draetta, Mol. Cell. Biol. 11:6177-6184, 1991). Those were mapped by building model based on the crystallographic coordinates catalytic subunit cyclic AMP-dependent (cAPK) (D. R. Knighton, J. Zheng, L. F. Ten Eyck, V. A. Ashford, N.-H. Xuong, S. Taylor, M. Sowadski,...