A5008748929- Cellular transport and secretion
- Endoplasmic Reticulum Stress and Disease
- Photosynthetic Processes and Mechanisms
- Enzyme Structure and Function
- Plant Reproductive Biology
- Lysosomal Storage Disorders Research
- Biochemical and Molecular Research
- RNA regulation and disease
- Enzyme Production and Characterization
- Seed Germination and Physiology
- Plant tissue culture and regeneration
- Mitochondrial Function and Pathology
- Lipid Membrane Structure and Behavior
- Legume Nitrogen Fixing Symbiosis
- Nonlinear Dynamics and Pattern Formation
- Adenosine and Purinergic Signaling
- Polysaccharides and Plant Cell Walls
- Pancreatic function and diabetes
- Erythrocyte Function and Pathophysiology
- Peptidase Inhibition and Analysis
- Growth and nutrition in plants
- Glycosylation and Glycoproteins Research
- Calcium signaling and nucleotide metabolism
University of Geneva
2023
Universitat de València
2015-2022
Instituto de Biomedicina de Valencia
2021-2022
National Center for Genetic Engineering and Biotechnology
2021
Endosomal sorting complex required for transport-III (ESCRT-III) participates in essential cellular functions, from cell division to endosome maturation. The remarkable increase of its subunit diversity through evolution may have enabled the acquisition novel functions. Here, we characterize a ESCRT-III copolymer initiated by Vps60. Membrane-bound Vps60 polymers recruit Vps2, Vps24, Did2, and Ist1, as previously shown Snf7. Snf7- Vps60-based filaments can coexist on membranes without...
The p24 family of proteins (also known as the TMED family) are key regulators protein trafficking along secretory pathway, but very little is about their functions in plants. A quadruple loss-of-function mutant affecting genes from δ-1 subclass p24δ subfamily (p24δ3δ4δ5δ6) showed alterations Golgi, suggesting that these play a role organization compartments early pathway Arabidopsis Loss p24δ-1 also induced accumulation K/HDEL receptor ERD2a (ER lumen protein-retaining A) at Golgi and...
COP (coat protein) I-coated vesicles mediate intra-Golgi transport and retrograde from the Golgi to endoplasmic reticulum. These form through action of small GTPase ADP-ribosylation factor 1 (ARF1) COPI heptameric protein complex (coatomer), which consists seven subunits (α-, β-, β′-, γ-, δ-, ε- ζ-COP). In contrast mammals yeast, several isoforms for coatomer subunits, with exception γ δ, have been identified in Arabidopsis. To understand role proteins plant biology, we characterized a...
The early secretory pathway involves bidirectional transport between the endoplasmic reticulum (ER) and Golgi apparatus is mediated by coat protein complex I (COPI)-coated II (COPII)-coated vesicles. COPII vesicles are involved in ER to meanwhile COPI mediate intra-Golgi retrograde from ER. key component of coatomer complex, that composed seven subunits (α/β/β'/γ/δ/ε/ζ). In Arabidopsis two genes coding for β-COP subunit have been identified, which result recent tandem duplication. Here we...
p24 proteins are a family of type-I membrane that cycle between the endoplasmic reticulum (ER) and Golgi apparatus via Coat Protein I (COPI)- COPII-coated vesicles. These have been proposed to function as cargo receptors, but identity putative cargos in plants is still elusive. We previously generated an Arabidopsis (Arabidopsis thaliana) quadruple loss-of-function mutant affecting genes from δ-1 subclass delta subfamily (p24δ3δ4δ5δ6 mutant). This also had reduced protein levels other was...
Abstract Glycosylphosphatidylinositol (GPI)-anchored proteins (GPI-APs) play an important role in a variety of plant biological processes including growth, stress response, morphogenesis, signaling, and cell wall biosynthesis. The GPI anchor contains lipid-linked glycan backbone that is synthesized the endoplasmic reticulum (ER) where it subsequently transferred to C-terminus containing signal peptide by transamidase. Once attached protein, lipid moieties are remodeled. In mammals yeast,...
Coat protein I (COPI) is necessary for intra-Golgi transport and retrograde from the Golgi apparatus back to endoplasmic reticulum. The key component of COPI coat coatomer complex, which composed seven subunits (α/β/β’/γ/δ/ε/ζ) recruited en bloc cytosol onto membranes. In mammals yeast, α- β’-COP WD40 domains mediate cargo-selective interactions with dilysine motifs present in canonical cargoes vesicles. contrast three isoforms (β’1-3-COP) have been identified Arabidopsis. To understand role...
Coat Protein I (COPI) consists of a complex (coatomer) formed by seven subunits (α-, β-, β'-, γ-, δ-, ε-, and ζ-COP) that is recruited to Golgi membranes form vesicles shuttle from the apparatus ER between stacks. Recently, it has been described loss function mutants two Arabidopsis β-COP genes, β1-COP β2-COP, showed increased sensitivity salt stress (NaCl). Using mixture either Na+ or Cl− salts, we have now found are specifically highly sensitive chloride ions.
Abstract Amongst the different clathrin structures in mammalian cells, bi-layered coat colocalizing with endosomal sorting complex required for transport (ESCRT)-0 remains one of most ambiguous. Despite being observed first time twenty years ago, their structure and how they are assembled unknown. Here, we reconstituted vitro ESCRT-0 assembly onto various types membranes. The protein HRS, a known adaptor on endosomes, was found to form condensates. These condensates spread into thin layer...
Lipid remodeling of Glycosylphosphatidylinositol (GPI) anchors is required for their maturation and may influence the localization function GPI-anchored proteins (GPI-APs). Maturation GPI-anchors well characterized in animals fungi but very little known about this process plants. In yeast, GPI-lipid occurs entirely at ER initiated by enzyme Bst1p (Post-Glycosylphosphatidylinositol Attachment to Proteins inositol deacylase 1 -PGAP1- mammals Arabidopsis ). Next, Per1p phospholipase 3 -PGAP3-...
ABSTRACT GPI-anchored proteins (GPI-APs) play an important role in a variety of plant biological processes including growth, stress response, morphogenesis, signalling and cell wall biosynthesis. The GPI-anchor contains lipid-linked glycan backbone that is synthesized the endoplasmic reticulum (ER) where it subsequently transferred to C-terminus containing GPI signal peptide by transamidase. Once anchor attached protein, lipid moieties are remodelled. In mammals yeast, this remodelling...