A5044263313- Ubiquitin and proteasome pathways
- Cancer-related Molecular Pathways
- Microtubule and mitosis dynamics
- Protein Tyrosine Phosphatases
- 14-3-3 protein interactions
- DNA Repair Mechanisms
- CRISPR and Genetic Engineering
- Genomics and Chromatin Dynamics
- RNA modifications and cancer
- Endoplasmic Reticulum Stress and Disease
- Fungal and yeast genetics research
- PI3K/AKT/mTOR signaling in cancer
- RNA Research and Splicing
- Genetics and Neurodevelopmental Disorders
- Epigenetics and DNA Methylation
- Fibroblast Growth Factor Research
- Animal Genetics and Reproduction
- Chromosomal and Genetic Variations
- Protein Kinase Regulation and GTPase Signaling
- TGF-β signaling in diseases
- Cancer-related gene regulation
- Click Chemistry and Applications
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- RNA and protein synthesis mechanisms
- Genomic variations and chromosomal abnormalities
Centre National de la Recherche Scientifique
2002-2023
Inserm
1989-2023
Université de Montpellier
2014-2023
Institut de Recherche en Cancérologie de Montpellier
2023
Centre de Recherche en Biologie cellulaire de Montpellier
1995-2017
Université Toulouse III - Paul Sabatier
1995-2004
Laboratoire de Biologie Cellulaire et Moléculaire du Contrôle de la Prolifération
1999-2004
Zero to Three
2003
Institut de Pharmacologie et de Biologie Structurale
1996-1999
Institut de Biologie Valrose
1997
A cascade of events is triggered upon the addition growth factor to quiescent mammalian cells, which ultimately restarts proliferation by inducing transition from G0/G1 S-phase. We have studied cyclin D1, a putative G1 cyclin, in normal diploid human fibroblasts. Cyclin D1 accumulated and reached maximum level before S-phase serum cells. The protein was localized nucleus, it disappeared nucleus as cells proceeded into Microinjection anti-cyclin antibodies or antisense plasmid prevented...
The cellular action of growth factors, among them basic fibroblast factor (bFGF), is mediated by their interaction with a cell surface receptor, but the mechanism transfer mitogenic (or other) signals to nucleus has not been identified. In this work, we show that bFGF translocated and accumulated in nucleolus. Furthermore, nucleolar localization correlated stimulation transcription ribosomal genes during G0----G1 transition induced alone adult bovine aortic endothelial cells (ABAE cells)....
In mammalian cells inhibition of the cdc2 function results in arrest G2-phase cell cycle. Several cdc2-related gene products have been identified recently and it has hypothesized that they control earlier cycle events. Here we studied relationship between activation one these homologs, cdk2 protein kinase, progression through cultured human fibroblasts. We found was activated specifically localized to nucleus during S phase G2. Microinjection affinity-purified anti-cdk2 antibodies but not...
Transcription of ribosomal RNA genes is generally accepted to correlate with cell growth. Using primary cultures adult bovine aortic endothelial (ABAE) cells, we have shown that transcription rDNA in confluent cells falls 5% the level growing cells. Protein kinase NII appears be a limiting factor promote isolated nuclei was detected by immunocytochemistry cytoplasm, and nucleoli while it no longer present The activity, nuclei, estimated endogenous phosphorylation specific substrate,...
In eukaryotes the activity of CDK1 (CDC2), a cyclin-dependent kinase that initiates structural changes culminate in segregation chromosomes at mitosis, is regulated by synergistic and opposing activities cascade kinases phosphatases. Dephosphorylation threonine 14 tyrosine 15 CDC25 phosphatases key step activation CDK1-cyclin B protein kinase. Little currently known about role regulation CDC25B. Here we report vitro vivo data indicate CDC25B degraded proteasome. This degradation dependent...
DNA topoisomerase II (TOP2) plays a crucial role in DNA-associated processes by inducing transient double-strand breaks, making it an important target for DNA-damage stabilizing agents. Commonly used cancer therapy, these agents are designed to interfere with TOP2 cleavage complexes on chromatin. However, the epigenetic pathways influencing their effectiveness and resultant cellular responses remain elusive. Here, we combine vitro as well vivo genetic pharmacological approaches prostate...
ABSTRACT Wee1 kinase-dependent phosphorylation of cdc2 maintains the cdc2/cyclin B complex in an inert form until it is activated by cdc25 tyrosine phosphatase at end G2. As described for cdc25, cell cycle-linked changes intracellular localisation wee1 may constitute important aspect temporal regulation activity. Here we report that subcellular distribution human during cycle HeLa and IMR90 cells. During interphase, found almost exclusively nucleus. When enters mitosis, relocalised into...
In eukaryotic cells, proteasomes play an essential role in intracellular proteolysis and are involved the control of most biological processes through regulated degradation key proteins. Analysis 20S proteasome localization human cell lines, using ectopic expression its CFP-tagged alpha7 subunit, revealed presence nuclear foci a specific proteolytically active complex made by association with PA28gamma regulator. Identification these as speckles (NS), which dynamic subnuclear structures...
Abstract CDC25 dual-specificity phosphatases are essential regulators that dephosphorylate and activate cyclin-dependent kinase/cyclin complexes at key transitions of the cell cycle. activity is currently considered to be an interesting target for development new antiproliferative agents. Here we report identification a inhibitor characterization its effects molecular cellular levels, in animal models. BN82002 inhibits phosphatase recombinant human CDC25A, B, C vitro. It impairs...
Significance The 20S proteasome is a key actor of the control protein levels and integrity in cells. To perform its multiple functions, it works with series regulators, among which nuclear complex called PA28γ. In particular, PA28γ participates regulation cell proliferation dynamics. We describe here characterization protein, PIP30/FAM192A, binds tightly to favors interaction while inhibiting association coilin, central component Cajal bodies. Thus, PIP30/FAM192A critically controls...
The growth-inhibitory protein p21WAF1/CIP1 is a potent inhibitor of various cyclin-dependent kinases, the expression which regulated at transcriptional level by p53-dependent and -independent mechanisms. We examined mRNA in 5 human ovarian-adenocarcinoma cell lines, 1 primary culture normal surface epithelium 17 ovarian-tumor specimens. In cells, was expressed ovarian epithelial cells high adenocarcinoma 2008 IGROV-1 lines. p21 WAF1/CIP1 undetectable levels NIH-OVCAR-3 SKOV-3 lines are...
Cyclin A2 is a key actor in cell cycle regulation. Its degradation mid-mitosis relies on the ubiquitin-proteasome system (UPS). Using high resolution microscopic imaging, we find that cyclin persists beyond metaphase. Indeed, identify novel A2-containing compartment forms dynamic foci. FRET and FLIM analyses show ubiquitylation takes place predominantly these foci before spreading throughout cell. Moreover, inhibition of autophagy proliferating cells induce stabilisation subset, while...
Abstract Regulation of the intracellular localisation its actors is one key mechanisms underlying cell cycle control. CDC25 phosphatases are activators Cyclin‐Dependent Kinases (CDK) that undergo nucleo‐cytoplasmic shuttling during and in response to checkpoint activation. Here we report protein kinase PKB/Akt phosphorylates CDC25B on serine 353, resulting a nuclear export‐dependent cytoplasmic accumulation phosphatase. Oxidative stress activates reproduces effect phosphorylation...