A5026066230- Mitochondrial Function and Pathology
- Frailty in Older Adults
- Nutrition and Health in Aging
- Genetics, Aging, and Longevity in Model Organisms
- Skin and Cellular Biology Research
- Microtubule and mitosis dynamics
- Redox biology and oxidative stress
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cystic Fibrosis Research Advances
- Ubiquitin and proteasome pathways
- ATP Synthase and ATPases Research
- Protein Tyrosine Phosphatases
- S100 Proteins and Annexins
- Neonatal Respiratory Health Research
- 14-3-3 protein interactions
- Advanced biosensing and bioanalysis techniques
- Cell Adhesion Molecules Research
- Cancer-related Molecular Pathways
- Immune Response and Inflammation
- Adipose Tissue and Metabolism
- Neurogenesis and neuroplasticity mechanisms
- Cellular transport and secretion
- Virology and Viral Diseases
- Genetics and Neurodevelopmental Disorders
- Rabies epidemiology and control
Centre National de la Recherche Scientifique
2002-2023
Université Toulouse III - Paul Sabatier
2010-2023
Centre de Recherches sur la Cognition Animale
2016-2023
Université de Toulouse
2010-2020
Centre de Biologie du Développement
2013-2015
Délégation Paris 5
2011
Inserm
2004-2011
Université Paris Cité
2011
Institut Necker Enfants Malades
2004
Laboratoire de Biologie Cellulaire et Moléculaire du Contrôle de la Prolifération
2000-2001
Neutrophil apoptosis is a highly regulated process essential for inflammation resolution, the molecular mechanisms of which are only partially elucidated. In this study, we describe survival pathway controlled by proliferating cell nuclear antigen (PCNA), factor involved in DNA replication and repairing cells. We show that mature neutrophils, despite their inability to proliferate, express high levels PCNA exclusively cytosol constitutively associated with procaspases, presumably prevent...
Mitochondrial dynamics control the organelle's morphology, with fusion leading to formation of elongated tubules and fission isolated puncta, as well mitochondrial functions. Recent reports have shown that disruptions contribute neurodegenerative diseases. Mutations inner membrane GTPase OPA1 are responsible for type 1 dominant optic atrophy, by mechanisms not fully understood. We show here in rodent cortical primary neurons, downregulation protein leads fragmented mitochondria become less...
Cystic fibrosis is a fatal human genetic disease caused by mutations in the CFTR gene encoding cAMP-activated chloride channel. It characterized abnormal fluid transport across secretory epithelia and chronic inflammation lung, pancreas, intestine. Because cystic (CF) pathophysiology cannot be explained solely dysfunction of transmembrane conductance regulator (CFTR), we applied proteomic approach (bidimensional electrophoresis mass spectrometry) to search for differentially expressed...
Because neutrophil apoptosis plays a key role in resolving inflammation, identification of proteins regulating survival should provide new strategies to modulate inflammation. Using proteomic approach, coronin-1 was identified as cytosolic protein cleaved during apoptosis. Coronin-1 is an actin-binding that can associate with phagosomes and NADPH oxidase, but its involvement currently unknown. In coronin-1-transfected PLB985 cells, overexpression did not modify the kinetics granulocyte...
Aging is the most important risk factor for onset of several chronic diseases and functional decline. Understanding interplays between biological aging biology loss as well integrating a function-centered approach to care pathway older adults are crucial steps towards elaboration preventive strategies (both pharmacological non-pharmacological) against severity burdensome conditions during aging. In order tackle these two challenges, ie, how both manipulation implementation (the Integrated...
The most common mutation in the cystic fibrosis transmembrane conductance regulator (CFTR) gene, ΔF508, causes retention of ΔF508-CFTR endoplasmic reticulum and leads to absence CFTR Cl<sup>–</sup> channels plasma membrane. retains some channel activity so increased expression membrane can restore secretion deficiency. Recently, curcumin was shown rescue localization function. In our previous work, keratin 18 (K18) network implicated trafficking. Here, we hypothesized that could a functional...
We have previously reported an increased expression of cytokeratins 8/18 (K8/K18) in cells expressing the F508del mutation cystic fibrosis transmembrane conductance regulator (CFTR). This is associated with colocalization CFTR and K18 vicinity endoplasmic reticulum, although this reversed by treating curcumin, resulting rescue F508del-CFTR. In present work, we hypothesized that (i) K8/K18 network may interact physically CFTR, (ii) interaction modify function. was immunoprecipitated from HeLa...
OPA1 mutations cause protein haploinsufficiency leading to dominant optic atrophy (DOA), an incurable retinopathy with variable severity. Up 20% of patients also develop extraocular neurological complications. The mechanisms that this or its syndromic forms are still unknown. After identifying oxidative stress in a mouse model the pathology, we sought determine consequences dysfunction on redox homeostasis.Mitochondrial respiration, reactive oxygen species levels, antioxidant defenses, and...
The Geroscience field focuses on the core biological mechanisms of aging, which are involved in onset age-related diseases, as well declines intrinsic capacity (IC) (body functions) leading to dependency. A better understanding how measure true age an individual or aging is essential step that may lead definition putative markers capable predicting healthy aging.The main objective INStitute for Prevention agIng and medicine Rejuvenative (INSPIRE) Platform initiative build a program research...
Cystic fibrosis (CF) is a genetic disease caused by mutations in the CF gene (cftr). Physiologically, characterized an abnormal chloride secretion epithelia due to dysfunction of mutated cystic transmembrane conductance regulator (CFTR). CFTR cAMP-dependent channel whose most frequent mutation, deltaF508, leads aberrantly folded protein which causes channel. However, growing number reports suggest that modifier genes and environmental factors are involved physiology CF. To identify proteins...
To favor their replication, viruses express proteins that target diverse mammalian cellular pathways. Due to the limited size of many viral genomes, such are endowed with multiple functions, which require targeting different subcellular compartments. One salient example is X protein Borna disease virus, expressed both at mitochondria and in nucleus. Moreover, we recently demonstrated mitochondrial neuroprotective. In this study, sought examine mechanisms whereby transits between compartments...
Aging is the major risk factor for development of chronic diseases. After decades research focused on extending lifespan, current efforts seek primarily to promote healthy aging. Recent advances suggest that biological processes linked aging are more reliable than chronological age account an individual’s functional status, i.e. frail or robust. It becoming increasingly apparent may be detectable as a progressive loss resilience much earlier appearance clinical signs frailty. In this...
Abstract Background The Integrated Care for Older People (ICOPE) approach was developed by the World Health Organization (WHO) aiming to shift traditional focus of care based on diseases a function- and person-centered approach, focused maintaining monitoring intrinsic capacity (IC). This study aimed investigate ability ICOPE screening tool identify older people with clinically meaningful impairments in IC domains. Methods cross-sectional analysis included 603 adults, participants (mean age...