A5061438971- Estrogen and related hormone effects
- Cancer, Hypoxia, and Metabolism
- Advanced Proteomics Techniques and Applications
- Mitochondrial Function and Pathology
- Mass Spectrometry Techniques and Applications
- RNA modifications and cancer
- Receptor Mechanisms and Signaling
- bioluminescence and chemiluminescence research
- Epigenetics and DNA Methylation
- Protein Kinase Regulation and GTPase Signaling
- Inflammatory mediators and NSAID effects
- Steroid Chemistry and Biochemistry
- Glutathione Transferases and Polymorphisms
- Metabolomics and Mass Spectrometry Studies
- Pharmacogenetics and Drug Metabolism
- Advanced Biosensing Techniques and Applications
- Retinoids in leukemia and cellular processes
- Pharmacological Receptor Mechanisms and Effects
- Cancer-related gene regulation
- Heat shock proteins research
- interferon and immune responses
- Enzyme Structure and Function
- Advanced Fluorescence Microscopy Techniques
- Cephalopods and Marine Biology
- Radiomics and Machine Learning in Medical Imaging
Charles Sturt University
2013-2023
Australian National University
1997-2023
Australian Cancer Research Foundation
2019-2023
New York Hospital Queens
2022
Columbia University
2022
NewYork–Presbyterian Hospital
2022
The University of Melbourne
2019
Carl Zeiss (Germany)
2003-2008
ProteoSys (Germany)
2003-2008
Indiana University
2008
Amyloid beta (Abeta) 1–42 oligomers accumulate in brains of patients with Mild Cognitive Impairment (MCI) and disrupt synaptic plasticity processes that underlie memory formation. Synaptic binding Abeta to several putative receptor proteins is reported inhibit long-term potentiation, affect membrane trafficking induce reversible spine loss neurons, leading impaired cognitive performance ultimately anterograde amnesia the early stages Alzheimer's disease (AD). We have identified a not...
Inhibitors of protein synthesis, such as anisomycin and cycloheximide, lead to superinduction immediate-early genes. We demonstrate that these two drugs activate intracellular signaling pathways involving both the mitogen-activated kinase (MAPK) stress-activated (SAPK) cascades. The activation either pathway correlates with phosphorylation c-fos regulatory transcription factor Elk-1. In HeLa cells, stabilizes mRNA when synthesis is inhibited only 50%. Under conditions, anisomycin, in...
Several growth factor- and calcium-regulated kinases such as pp90 <sup>rsk</sup> or CaM kinase IV can phosphorylate the transcription factor serum response (SRF) at serine 103 (Ser-103). However, it is unknown whether stress-regulated also SRF. We show that treatment of cells with anisomycin, arsenite, sodium fluoride, tetrafluoroaluminate induces phosphorylation SRF Ser-103 in both HeLa NIH3T3 cells. This dependent on p38/SAPK2 correlates activation MAPKAP 2 (MK2). MK2 phosphorylates...
Abstract Introduction Breast tumors lacking the estrogen receptor-α (ER-α) have increased incidence of resistance to therapy and poorer clinical prognosis. Methods Whole tissue sections from 16 cryopreserved breast cancer that were either positive or negative for ER (eight eight negative) differentially analyzed by multiplex imaging two-dimensional PAGE gels using 54 cm isoelectric focusing. Differentially detected spots Progesterone Receptor Membrane Component 1 (PGRMC1) shown differ in...
Abstract Automated and unbiased methods of non-invasive cell monitoring able to deal with complex biological heterogeneity are fundamentally important for biology medicine. Label-free imaging provides information about endogenous autofluorescent metabolites, enzymes cofactors in cells. However extracting high content from autofluorescence has been hitherto impossible. Here, we quantitatively characterise populations different tissue types, live or fixed, by using novel image processing a...
Intracellular reactive oxygen intermediate (ROI) levels play an important role in numerous physiological and pathophysiological conditions. Apart from causing oxidative stress damage, ROI changes differentially activate gene expression. However the proto‐oncogene encoding AP‐1 transcription factor subunit c‐Fos is induced by both prooxidants antioxidants. Here, Elk‐1 identified as being responsible for c‐ fos serum response element (SRE) induction to cellular redox status treatment with...
Measurement of endogenous free and bound NAD(P)H relative concentrations in living cells is a useful method for monitoring aspects cellular metabolism, because the NADH/NAD+ reduction-oxidation pair crucial electron transfer through mitochondrial transport chain. Variations ratio are also implicated bioenergetic biosynthetic metabolic changes accompanying cancer. This study uses two-photon fluorescence lifetime imaging microscopy (FLIM) to investigate MCF10A premalignant breast cancer...
Progesterone Receptor Membrane Component 1 (PGRMC1) is expressed in many cancer cells, where it associated with detrimental patient outcomes. It contains phosphorylated tyrosines which evolutionarily preceded deuterostome gastrulation and tissue differentiation mechanisms.
Progesterone influences mammary gland development and probably breast cancer tumorigenesis functions by regulating a broad spectrum of physiological processes. We investigated receptor membrane-initiated actions progesterone in MCF-7 cells via membrane component 1 (PGRMC1).The expression PGRMC1 was verified immune fluorescent analysis paraffin sections. were transfected with (wild type) or variants. These stimulated membrane-impermeable (P4) conjugate (P4-BSA-fluorescein isothiocyanate,...
Evidence is accumulating that progestogens may play a crucial role in the development of breast cancer under contraception and hormone therapy reproductive menopausal women. Progesterone receptor membrane component 1 (PGRMC1) expressed be important tumorigenesis thus increase risk. The aim this project was to investigate influence progesterone nine synthetic progestins on MCF-7 cells overexpressing PGRMC1.MCF-7 were stably transfected with PGRMC1 expression plasmid (WT-12). To test effects...
Progesterone receptor membrane component 1 (PGRMC1) is a multifunctional protein implicated in multiple pathologies, including cancer and Alzheimer's disease. The recently published structure of PGRMC1 revealed heme-mediated dimerization that directed the PGRMC1-dependent cytochrome P450-mediated detoxification doxorubicin. We describe here how also enables important new insights into possible regulation function by phosphorylation. Predicted regulatory interaction sites for SH2- SH3-domain...
Abstract The presence of progesterone receptor (PR) in estrogen (ER)‐positive breast cancer is associated with a good prognosis, and indicates that tumors are likely to respond tamoxifen. However, ER + /PR − less well. To reveal the potential molecular mechanism this phenomenon, we sought identify differential protein abundances between invasive ductal carcinoma cells from cryopreserved mammary tumor specimens. Because current proteomics methods hampered examination most primary human...
The activity of mitochondria induces, as a byproduct, variety post-translational modifications in associated proteins, which have functional downstream consequences for processes such apoptosis, autophagy, and plasticity; e.g., reactive oxygen species (ROS), induce N-formyl-kynurenine from oxidized tryptophans certain mitochondrial proteins are localized close spatial proximity to their source. This type fast molecular changes has profound influence on cell death survival with implications...
Abstract Purpose: To identify pretherapeutic predictive biomarkers in tumor biopsies of patients with locally advanced esophageal adenocarcinomas treated neoadjuvant chemotherapy, we used an explorative proteomic approach to correlate protein expression profiles response chemotherapy. Experimental Design: Thirty-four who received platin/5-fluorouracil–based chemotherapy before surgical resection were enrolled this study. Response was determined (a) by the amount decline...
A differential quantitative protein expression study, comparing matched prostate cancerous and benign tissues from 31 patients, revealed proteins newly associated with cancer. Average effects for 17 whose abundance was significantly different (p<0.01) across patients ranged 1.5- to 6.1-fold, included a number of known cancer markers. The most differentially abundant between samples were isopeptidase T, serum amyloid P (SAP), annexin A3 (ANXA3) mitochondrial enoyl coenzyme-A hydratase. SAP is...
ABSTRACT Isolated tube feet of Strongylocentrotus franciscanus contract briefly when the outer epithelium is touched. Similar twitch-like contractions can be induced by electrical stimulation surface foot. These responses appear to chemically mediated. The following evidence indicates that transmitter substance may acetylcholine (ACh): ACh causes muscle contraction. This effect and stimuli potentiated anticholinesterase agents antagonized cholinergic blocking agents. Anaesthesia with...
In Brief Objective: Breast cancer risk is still an important topic regarding hormone therapy as well oral contraception. Evidence that progestogens may play a crucial role accumulating. Progesterone receptor membrane component 1 (PGRMC1) expressed in breast be tumorigenesis and thus increase risk. The aim of this project was to investigate the influence different estradiol (E2) concentrations addition two on MCF-7 cells overexpressing PGRMC1. Methods: were stably transfected with PGRMC1...
A complex PGRMC1-centred regulatory system controls multiple cell functions. Although PGRMC1 is phosphorylated at several positions, we do not understand the mechanisms regulating its function. archetypal member of membrane associated progesterone receptor (MAPR) family. Phylogentic comparison MAPR proteins suggests that ancestral metazoan "PGRMC-like" gene resembled PGRMC1/PGRMC2, containing equivalents Y139 and Y180 SH2 target motifs. It later acquired a CK2 site with phosphoacceptor S181....