A5020482386- Advanced Fluorescence Microscopy Techniques
- Optical Coherence Tomography Applications
- Spectroscopy Techniques in Biomedical and Chemical Research
- Near-Field Optical Microscopy
- Digital Holography and Microscopy
- Glaucoma and retinal disorders
- Photoreceptor and optogenetics research
- Urinary Tract Infections Management
- Corneal surgery and disorders
- Photodynamic Therapy Research Studies
- Advanced Optical Sensing Technologies
- Laser Material Processing Techniques
- Orbital Angular Momentum in Optics
- Cell Image Analysis Techniques
- Metamaterials and Metasurfaces Applications
- Microfluidic and Bio-sensing Technologies
- CCD and CMOS Imaging Sensors
- RFID technology advancements
- Chemical Reactions and Mechanisms
- Optical Coatings and Gratings
- Laser Applications in Dentistry and Medicine
- Retinal Diseases and Treatments
- Image Enhancement Techniques
- Advanced Neural Network Applications
- Nanofabrication and Lithography Techniques
Nanyang Technological University
2015-2020
Macquarie University
2008-2016
Bellingham Technical College
2015
Holst Centre (Netherlands)
2013-2014
Cochin University of Science and Technology
2007
Abstract Automated and unbiased methods of non-invasive cell monitoring able to deal with complex biological heterogeneity are fundamentally important for biology medicine. Label-free imaging provides information about endogenous autofluorescent metabolites, enzymes cofactors in cells. However extracting high content from autofluorescence has been hitherto impossible. Here, we quantitatively characterise populations different tissue types, live or fixed, by using novel image processing a...
Abstract Direct visualization and assessment of the iridocorneal angle (ICA) region with high resolution is important for clinical evaluation glaucoma. However, current imaging systems ICA do not provide sufficient structural details due to their poor resolution. The key challenges in achieving quality are its location anterior eye occurrence total internal reflection refractive index difference between cornea air. Here, we report an indirect axicon assisted gonioscopy probe white light...
Abstract Background and Objective The photobiological effect of laser light on cells tissues originates from absorption by endogenous chromophores hence it depends the wavelength source cell type. Earlier studies regarding biostimulation effects green investigated a wide variety but not adipose tissue‐derived stem (ADSCS). In this study we reported in vitro 532‐nm Nd:YAG proliferation, mitochondrial activity these mesenchymal (MSCs) autofluorescence emission at wavelengths associated with...
Abstract An optical imaging probe with targeted multispectral and spatiotemporal illumination features has applications in many diagnostic biomedical studies. However, these systems are mostly adapted conventional microscopes, limiting their use for vitro applications. We present a variable resolution using digital micromirror device (DMD) an achievable maximum lateral of 2.7 μm axial 5.5 μm, along precise shape selective ability. have demonstrated switching different wavelengths to image...
Laser induced forward transfer is an emerging material deposition technology. We investigated the feasibility of this technique for printing thermally sensitive, electrically conductive adhesives with and without using intermediate dynamic release layer. A 248 nm KrF-excimer laser was used to print epoxy-based containing silver flakes down 75 μm dot size. The process particularly relevant realizing electrical connections surface mount devices in microelectronics industry. Characterization...
Optical fiber based laser induced fluorescence (LIF) measurements were carried out using Rhodamine B to analyze two different species of bacteria, a Gram-positive bacteria namely Bacillus smithii , and Vibrio alginolyticus, Gram-negative bacteria. The sensor was clearly able distinguish between the Quenching effect dye by observed. on samples also studied in detail.
Breaking the diffraction limit in imaging microscopes with far-field options has always been thrust challenge for optical engineers and biologists over years. Although structured illumination microscopy Bessel beam assisted shown capability of sub-diffraction resolutions, they rely on use objective lenses large numerical apertures (NA). Hence, fail to sustain resolutions at larger working distances. In this context, we demonstrate a method nanoscale resolution longer distances, named as...
Imaging of physically inaccessible parts the body such as colon at micron-level resolution is highly important in diagnostic medical imaging. Though flexible endoscopes based on imaging fiber bundle are used for procedures, their inherent honeycomb-like structure creates pixelation effects. This impedes observer from perceiving information an image captured and hinders direct use processing machine intelligence techniques recorded signal. Significant efforts have been made by researchers...
We report a simple real time optical imaging concept using an axicon lens to image the object kept behind opaque obstacles in free space. The proposed underlines importance and advantages of compared conventional obstacle. potential this is demonstrated by insertion surgical needle biological specimen time, without blocking field view. It envisaged that concepts methodology can make telling impact wide variety areas especially for diagnostics, therapeutics microscopy applications.
Flexible fiber optic imaging systems including confocal probes have found tremendous significance in the recent past for its applications high resolution imaging. However, motorized stage is required scanning sample or tip of based probes. In this context, we propose a probe system using digital spatial light modulator devoid mechanical stage. Each fiberlet image acts not only as conduit but also pinhole. The paper introduces variation contrast by varying number illuminated fiberlets which...
There was a renewed interest, during the recent years, in imaging and tracking of targeted cells or organelles for variety biomedical lab-on chip applications that include particles movement. However, nonspecific illumination can have adverse effects such as heating, reduced image contrast photo bleaching. In fact, current available systems are unable to selectively illuminate particle being tracked. To fill this void, we developed fiber optics based probe system incorporating spatial light...
In this paper, the first results of Laser-Induced Forward Transfer (LIFT)-assisted printing micro-bumps non-flux based silver nanoparticle (AgNP) and flux-based solder pastes for flip-chip bonding optoeletronic components were reported . The successful demonstration chip-bumping with AgNP using LIFT shows great potential technique interconnect technology especially applications requiring fine pitch high accuracy.
It is well known for structured illumination microscopy (SIM) that the lateral resolution by a factor of two beyond classical diffraction limit achieved using spatially in wide-field fluorescence microscope. In state art SIM systems, grating patterns are generally generated physical gratings or spatial light modulators such as digital micro mirrors (DMD), liquid crystal displays (LCD). this study, combination LCD and ground glasses, size controlled randomized speckle an source Proof concept...
Automated and unbiased methods of non-invasive cell monitoring able to deal with complex biological heterogeneity are fundamentally important for biology medicine. Label-free imaging provides information about endogenous fluorescent metabolites, enzymes cofactors in cells. However extracting high content from native fluorescence has been hitherto impossible. Here, we quantitatively characterise populations different tissue types, live or fixed, by using novel image processing a simple...
Time averaged imaging is one of the widely used methods to achieve improved image quality, in different types microscopic methods. refers adjusting exposure time system obtain optimal images. In state art microscopes, region interest (ROI) illumination beam for can be selected regular shapes such as circle or rectangle. This forces smallest possible ROI larger than actual sample’s which a specific shape with complex contours. this context, we present flexible fiber bundle based probe capable...
Axicon lenses are conical prisms, which known to focus a light source line comprising of multiple points along the optical axis. In this study, we analyze potential axicon view, image and record object behind opaque obstacles in free space. The advantage an lens over regular is demonstrated experimentally. Parameters such as obstacle size, position context imaging tested using Zemax simulation. This proposed concept can be easily adapted most methods microscopy modalities.
Spatially non-uniform illumination patterns have shown significant potential to improve the imaging. Recent developments in patterned microscopy demonstrated that use of an optical speckle as pattern significantly improves imaging resolution at same time reducing computational overheads. We present a DMD based method for generation digital pattern. The generated and uniform white light are used two illuminations acquire images. image reconstruction algorithm blind structured is get high...
Current clinical optical imaging systems do not provide sufficient structural information of trabecular meshwork (TM) in the iridocorneal angle (ICA) eye due to their low resolution. Increase intraocular pressure (IOP) can occur abnormalities TM, which could subsequently lead glaucoma. Here, we present an indirect gonioscopy based probe with significantly improved visualization structures ICA including TM region, compared currently available tools. Imaging quality developed system was tested...