A5091797746- Gastric Cancer Management and Outcomes
- Esophageal Cancer Research and Treatment
- Gastrointestinal Tumor Research and Treatment
- Cancer-related gene regulation
- Radiomics and Machine Learning in Medical Imaging
- Pancreatic and Hepatic Oncology Research
- Cancer Immunotherapy and Biomarkers
- Metastasis and carcinoma case studies
- Genetic factors in colorectal cancer
- Lung Cancer Treatments and Mutations
- Colorectal Cancer Surgical Treatments
- HER2/EGFR in Cancer Research
- Autophagy in Disease and Therapy
- RNA modifications and cancer
- Colorectal Cancer Treatments and Studies
- Monoclonal and Polyclonal Antibodies Research
- Cancer, Hypoxia, and Metabolism
- Esophageal and GI Pathology
- Advanced Biosensing Techniques and Applications
- Colorectal and Anal Carcinomas
- Heat shock proteins research
- Molecular Biology Techniques and Applications
- Colorectal Cancer Screening and Detection
- Epigenetics and DNA Methylation
- Sarcoma Diagnosis and Treatment
Kepler Universitätsklinikum
2021-2025
Johannes Kepler University of Linz
2020-2025
Klinikum rechts der Isar
2005-2023
University of Bern
2013-2022
University Hospital of Bern
2016-2022
Technical University of Munich
2007-2016
Krankenhaus der Elisabethinen
2015
Weatherford College
2013
Semmelweis University
2010-2013
Taipei Institute of Pathology
2010-2012
In Brief Objective: An increasing number of patients with locally advanced gastric carcinomas (GC) are being treated preoperative chemotherapy before surgery. Background: Histopathological tumor regression may have an important prognostic impact in addition to the UICC-TNM classification system. Methods: We evaluated histopathological 480 surgical resection specimens GC after neoadjuvant cisplatin-based chemotherapy, using established system encompassing three grades based on estimation...
BackgroundNeoadjuvant chemotherapy before surgery improves survival compared with alone for patients oesophageal cancer. The OE05 trial assessed whether increasing the duration and intensity of neoadjuvant further improved current standard regimen.MethodsOE05 was an open-label, phase 3, randomised clinical trial. Patients surgically resectable adenocarcinoma classified as stage cT1N1, cT2N1, cT3N0/N1, or cT4N0/N1 were recruited from 72 UK hospitals. Eligibility criteria included WHO...
Deep learning (DL) can accelerate the prediction of prognostic biomarkers from routine pathology slides in colorectal cancer (CRC). However, current approaches rely on convolutional neural networks (CNNs) and have mostly been validated small patient cohorts. Here, we develop a new transformer-based pipeline for end-to-end biomarker by combining pre-trained transformer encoder with network patch aggregation. Our approach substantially improves performance, generalizability, data efficiency,...
Microsatellite instability (MSI)/mismatch repair deficiency (dMMR) is a key genetic feature which should be tested in every patient with colorectal cancer (CRC) according to medical guidelines. Artificial intelligence (AI) methods can detect MSI/dMMR directly routine pathology slides, but the test performance has not been systematically investigated predefined thresholds.
Chemotherapy for advanced colorectal cancer leads to improved survival; however, predictors of response systemic treatment are not available. Genomic and epigenetic alterations the gene encoding transcription factor AP-2 epsilon (TFAP2E) common in human cancers. The dickkopf homolog 4 protein (DKK4) is a potential downstream target TFAP2E has been implicated chemotherapy resistance. We aimed further evaluate role DKK4 as chemotherapy.
Previous studies demonstrated that chemotherapy-induced changes in tumor glucose metabolism measured with <sup>18</sup>F-FDG PET identify patients who benefit from preoperative chemotherapy and those do not. The prognosis for metabolic nonresponders is poorer than responders. Therefore, we initiated this prospective trial to improve the clinical outcome of using a salvage neoadjuvant radiochemotherapy. <b>Methods:</b> Fifty-six locally advanced adenocarcinomas esophagogastric junction were...
// Melanie Straub 1 , Enken Drecoll Nicole Pfarr Wilko Weichert 1,5,6 Rupert Langer 2 Alexander Hapfelmeier 3 Carolin Götz 4 Klaus-Dietrich Wolff Andreas Kolk and Katja Specht Institute of Pathology, Technical University Munich, Germany Bern, Switzerland Medical Statistics Epidemiology, Department Oral Maxillofacial Surgery, Klinikum rechts der Isar, 5 National Center Tumor Diseases (NCT), Heidelberg, 6 German Cancer Consortium (DKTK), Correspondence to: Specht, email: Keywords : PD-L1,...
Autophagy assures cellular homeostasis, and gains increasing importance in cancer, where it impacts on carcinogenesis, propagation of the malignant phenotype development resistance. To date, its tissue-based analysis by immunohistochemistry remains poorly standardized. Here we show feasibility specifically reliably assessing autophagy markers LC3B p62 (SQSTM1) formalin fixed paraffin embedded human tissue immunohistochemistry. Preceding functional experiments consisted depleting H1299 lung...
Abstract We present the first analytical approach to demonstrate in situ imaging of metabolites from formalin‐fixed, paraffin‐embedded ( FFPE ) human tissue samples. Using high‐resolution matrix‐assisted laser desorption/ionization Fourier‐transform ion cyclotron resonance mass spectrometry MALDI‐FT‐ICR MSI ), we conducted a proof‐of‐principle experiment comparing metabolite measurements and fresh frozen sections, found an overlap 72% amongst 1700 m/z species. In particular, observed...
We analyzed the long-term outcome of patients operated for esophageal cancer and evaluated new seventh edition tumor-node-metastasis classification cancers esophagus.Retrospective analysis classification.Data a single-center cohort 2920 esophagus according to are presented. Statistical methods evaluate survival prognostic performance staging systems included Kaplan-Meier analyses time-dependent receiver-operating-characteristic-analysis.Union Internationale Contre le Cancer stage, R-status,...
In clinical diagnostics, it is of outmost importance to correctly identify the source a metastatic tumor, especially if no apparent primary tumor present. Tissue-based proteomics might allow correct classification. As result, we performed MALDI imaging generate proteomic signatures for different tumors. These were used classify common cancer types. At first, cohort comprised tissue samples from six adenocarcinoma entities located at organ sites (esophagus, breast, colon, liver, stomach,...
Abstract Regional lymph node metastasis negatively affects prognosis in colon cancer patients. The molecular processes leading to regional are only partially understood and proteomic markers for still scarce. Therefore, a tissue‐based approach was undertaken identifying proteins associated with metastasis. Two complementary methods have been employed. MALDI imaging used small (≤25 kDa) situ label‐free quantitative proteomics larger proteins. A tissue cohort comprising primary tumours without...
Targeting of the HER2 protein in human breast cancer represents a major advance oncology but relies on measurements total and not signaling network activation. We used reverse-phase microarrays (RPMA) to measure phosphorylated context HER family understand correlations between levels downstream activity.Three independent study sets, comprising 415 individual patient samples from flash-frozen core biopsy formalin-fixed paraffin-embedded (FFPE) surgical samples, were analyzed via RPMA. The...
// Anna M. Schläfli 1 , Olivia Adams 1,2 José A. Galván Mathias Gugger 1,3 Spasenija Savic 4 Lukas Bubendorf Ralph Schmid 5 Karl-Friedrich Becker 6 Mario P. Tschan Rupert Langer and Sabina Berezowska Institute of Pathology, University Bern, Switzerland 2 Graduate School for Cellular Biomedical Sciences, 3 Promed Laboratoire Médical, Marly, Universty Hospital Basel, Division General Thoracic Surgery, Inselspital Technische Universität München, Germany Correspondence to: Berezowska, email:...
Recently, histopathological tumour regression, prevalence of signet ring cells, and localisation were reported as prognostic factors in neoadjuvantly treated oesophagogastric (junctional gastric) cancer. This exploratory retrospective study analyses independent within a large patient cohort after preoperative chemotherapy including clinical factors.In all, 850 patients presenting with cancer staged cT3/4 Nany cM0/x neoadjuvant followed by resection two academic centres. Patient data...